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Mesenchymal stem cell injection, as well as preparation method and application in preparing medicament for treating children dilated cardiomyopathy

A technology of stem cells and injections, applied in the field of biomedicine, can solve the problems of limited clinical application, ethical issues, limited sources, etc., and achieve the effect of getting rid of the limitation of life activities, improving the quality of life and increasing the output

Inactive Publication Date: 2013-02-06
青岛奥克生物开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In theory, embryonic stem cells and induced pluripotent differentiated stem cells are ideal seed cells for cellular cardiomyoplasty in children with cardiomyopathy; however, the source of the former is limited and may have ethical issues; while the latter requires additional induction during the culture process. The effect of the substance and the culture medium itself on the seed cells and the cardiomyocytes has not yet been clarified, so its clinical application is greatly limited, and most of them are limited to animal experiments

Method used

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  • Mesenchymal stem cell injection, as well as preparation method and application in preparing medicament for treating children dilated cardiomyopathy
  • Mesenchymal stem cell injection, as well as preparation method and application in preparing medicament for treating children dilated cardiomyopathy
  • Mesenchymal stem cell injection, as well as preparation method and application in preparing medicament for treating children dilated cardiomyopathy

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Embodiment 1

[0028] 1. Preparation of umbilical cord mesenchymal stem cells

[0029] 1. Fresh full-term healthy fetal umbilical cord is washed with PBS buffer; the PBS buffer contains 100kU / L penicillin and 100mg / L streptomycin;

[0030] 2. Cut the 3-15cm long umbilical cord, cut the umbilical cord into 1-2cm long segments, and wash it repeatedly with PBS buffer; the PBS buffer contains 100kU / L penicillin and 100mg / L streptomycin;

[0031] 3. Cut the umbilical cord tissue pieces into 5mm 3 Small piece;

[0032] 4. Add the chopped tissue to L-DMEM medium for washing, centrifuge at 500-700g for 5 minutes, and discard the supernatant;

[0033] 5. Mix the tissue block and the medium according to the volume ratio of 2-3:1, inoculate them into cell culture dishes, and culture them in an incubator; the medium is a serum-free medium for MSC;

[0034] 6. Change the medium every 3 days, and subculture when the cells reach about 80% confluence; the subculture medium is a serum-free medium for MSC....

Embodiment 2

[0068] For example, 100ml of stem cell injection is prepared, which consists of human mesenchymal stem cells, 8ml of clinical grade DMSO, 30ml of human serum albumin stock solution (concentration of the mass volume ratio of the stock solution is 20%), and 62ml of Bomaili (compound electrolyte solution). The number of mesenchymal stem cells per milliliter injection is 2×10 5 -1×10 7 . Mesenchymal stem cell injection can still maintain a single-cell suspension state at an ambient temperature of -196°C, and the cell viability remains above 85% after resuscitation, which can be directly used for clinical injection. The mass-to-volume ratios in the present invention all represent the ratio of mass (g) to volume (ml).

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Abstract

The invention provides mesenchymal stem cell injection, as well as a preparation method and application in preparing a medicament for treating children dilated cardiomyopathy. The mesenchymal stem cell injection comprises 2*10<5>-1*10<7> mesenchymal stem cells in every milliliter, clinical grade DMSO (dimethylsulfoxide) with volume ratio of 5 to 8 percent, human albumin with mass volume ratio of 1 to 6 percent, and multi-electrolyte liquid. The mesenchymal stem cell injection is prepared from placenta and umbilical cord, has the advantages of being high in yield, and being easy to realize industrialization, the preparation system quality is easy to control; the injection can be directly frozen through program-controlled freezing procedure and directly used in clinical injection after being recovered, and is safe and reliable. According to the injection, the dilated cardial structure can be changed, the function of heart can be recovered, the water supply state of each tissue in the whole body can be improved, the living quality of a patient can be improved, and the dilated cardiomyopathy can be treated fundamentally, so that the patient can get rid of serious complications caused by massive medicine administration, living action limitation and poor disease control.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a mesenchymal stem cell injection, a preparation method thereof, and an application in preparing a medicine for treating children with dilated cardiomyopathy. Background technique [0002] Dilated cardiomyopathy is the expansion of one or both sides of the heart chamber accompanied by myocardial hypertrophy, myocardial systolic pump dysfunction, resulting in congestive heart failure. This type is characterized by enlargement of the left or right ventricle or bilateral ventricle, accompanied by myocardial hypertrophy. Ventricular hyposystole, with or without congestive heart failure. The condition is progressively exacerbated, and death can occur at any stage of the disease. Ventricular or atrial arrhythmia is more common, and it is the first or main manifestation, and multiple arrhythmias coexist to form a relatively complex heart rhythm, which can occur repeatedly. A hi...

Claims

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Application Information

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IPC IPC(8): A61K35/44A61K35/50A61K38/38A61K9/10A61P9/04C12N5/0775
CPCA61K35/44A61K47/42C12N5/0668A61K35/50C12N5/0665A61P9/04
Inventor 魏斯溧高宏王丽胡建霞张学峰
Owner 青岛奥克生物开发有限公司
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