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Human IgG4 (immunoglobulin G4) antibody adsorption column and preparation method and application thereof

An adsorption column and antibody technology, which is applied in the field of medical devices, can solve the problems of inability to efficiently purify human IgG4 antibodies, and achieve specific purification, cost saving, and high economic benefits

Inactive Publication Date: 2016-10-26
HOSPITAL AFFILIATED TO GUANDONG MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] One of the purposes of the present invention is to provide an adsorption column for human IgG4 antibody to solve the problem of inability to efficiently purify human IgG4 antibody in the prior art

Method used

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  • Human IgG4 (immunoglobulin G4) antibody adsorption column and preparation method and application thereof
  • Human IgG4 (immunoglobulin G4) antibody adsorption column and preparation method and application thereof
  • Human IgG4 (immunoglobulin G4) antibody adsorption column and preparation method and application thereof

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specific Embodiment 1

[0031] Refer to attached figure 1 The adsorption column for human IgG4 antibody involved in this embodiment includes a column body 1, the two ends of the column body 1 are closed, a connecting tube 2 is respectively provided at the closed ends of the column body 1, and a connecting tube 2 is provided in the inner cavity of the column body 1. There is an adsorbent, and the adsorbent is composed of a ligand and a carrier, the ligand is an anti-human IgG4 antibody, and the carrier is agarose microspheres, polystyrene microspheres or magnetic beads.

[0032] In this example:

[0033] The cylinder 1 is in the shape of a cylinder. The anti-human IgG4 antibody is a mouse anti-human IgG4 antibody. The carrier is aminated agarose microspheres.

[0034] The preparation method of an adsorption column for human IgG4 antibody involved in this embodiment is characterized in that it comprises the following steps,

[0035] 1) making and forming a cylinder 1 so that the cylinder 1 is in th...

specific Embodiment 2

[0051] The characteristics of this embodiment are:

[0052] The cylinder 1 is in the shape of an elliptical cylinder or a polygonal cylinder. The anti-human IgG4 antibody can be one or a combination of two or more of human anti-human IgG4 antibody, rabbit anti-human IgG4 antibody, donkey anti-human IgG4 antibody or goat anti-human IgG4 antibody. The carrier is aminated or carboxylated agarose microspheres, or the carrier is amino or carboxylated polystyrene microspheres; or the carrier is aminated or carboxylated magnetic beads.

[0053] The specific method for preparing the human IgG4 antibody adsorption column is as follows:

[0054] Using the prepared aminated magnetic beads (SiO2 coated Fe3O4) as the adsorption material, mix the rabbit anti-human IgG4 antibody (final concentration 3mg / mL) with the magnetic beads in a 0.1mol / L NaHCO3 solution containing 0.5mol / L NaCl , react at room temperature for 12 hours (mix once every 1 hour), separate the magnetic beads with a magne...

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Abstract

The invention relates to a human IgG4 (immunoglobulin G4) antibody adsorption column and a preparation method and application thereof. The human IgG4 antibody adsorption column comprises a column body and is characterized in that two ends of the column body are closed, a connection pipe is arranged at each of the two closed end of the column body, an adsorbent is filled in an inner cavity of the column body and consists of a ligand and a carrier, an anti-human IgG4 antibody serves as the ligand, the carrier is selected from agarose microspheres, polystyrene microspheres or magnetic beads, and the column body is in a shape of a cylinder, an elliptical cylinder or a polygonal column. The preparation method includes: sealing one end of the column body, arranging one of the connection pipes at the sealed end, and filling the absorbent into the inner cavity of the column body; sealing the other end of the column body, arranging the other connection pipe at the sealed end to obtain the human IgG4 antibody adsorption column. Purification of human IgG4 from human blood is realized by the aid of the human IgG4 antibody adsorption column, and recycling of the human IgG4 antibody adsorption column is realized. The human IgG4 antibody adsorption column has advantages of high efficiency and specificity in human IgG4 antibody purification and lays a foundation for IgG4 research and application.

Description

technical field [0001] The invention relates to an adsorption column of human IgG4 antibody, a preparation method and application thereof. It belongs to the technical field of medical equipment. Background technique [0002] Immunoglobulin G (Immunoglobulin G, IgG) is the main part of immunoglobulin in serum (about 75% of Ig), divided into four subtypes: IgG1 (60%-70%), IgG2 (15%-20%) %), IgG3 (5%-10%), IgG4 (4%-6%), and there are differences in the immunological characteristics and functions of each subtype (Shakib F.The human IgG subclasses: molecular analysis of structure, function and regulation .4th ed. Elsevier, 2013). IgG4 is one of the four subtypes of IgG antibodies. Although the content is small (adult: 0.08-1.40g / L), it is the most biologically unique: ①The unique structure of the IgG4 hinge region (Arch Biochem Biophys, 2012 ,526(2):159-66), which determines its significant lack of ability to activate complement C1q and bind FcγRs (J Exp Med,1991,173(4):1025-8...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/24B01J20/26B01J20/30C07K16/06C07K1/22
CPCB01J20/24B01J20/265C07K1/22C07K16/065
Inventor 潘庆军刘华锋郭林杰吴平廖焕金吴静蔡珺彭艳霞张莉芳
Owner HOSPITAL AFFILIATED TO GUANDONG MEDICAL COLLEGE
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