Synthetic method used for preparing adsorbent for clearing pathogenic antibody by oxidizing periodate

Abstract

The invention relates to a synthetic method used for preparing an adsorbent for clearing a pathogenic antibody by oxidizing periodate, and discloses a method for preparing a protein immunoadsorption material by oxidizing the periodate by taking agarose gel as a carrier. The method comprises the following steps of: preparing aldehyde group-containing agarose gel by oxidizing the agarose gel by using the periodate; coupling the aldehyde group-containing agarose gel and immunoglobulin binding protein; and blocking a material and reducing or reducing directly without blocking to obtain the immunoadsorption blood purification material. The method has the characteristics of simple process, environmental friendliness, low cost and the like; and simultaneously the product has high specificity, adsorptive property, regenerability and the like, and can be used for clinical immunoadsorption treatment practically.

Description

technical field

[0001] The invention relates to a synthesis method for preparing an adsorbent for eliminating pathogenic antibodies by oxidation of periodate, and belongs to the fields of biomedical materials and blood purification. Background technique

[0002] Immunoadsorption (IA) therapy is a new blood purification technology developed in the past 15 years, and it is used to treat some diseases that traditional methods are ineffective. The basic principle is to connect antigens, antibodies or some substances with specific affinity as ligands to the carrier, prepare an in vitro adsorption column for in vitro blood purification, and use its specific adsorption properties to selectively or specifically remove Endogenous pathogenic factors in the patient's blood, so as to achieve the purpose of purifying the blood and relieving the disease. In 2001, the first European Immunoadsorption Symposium was held in London, England. More than 200 experts and scholars from 17 countrie...

Images