Quick detection method of tea polyphenol contents on basis of micro-fluidic paper chip technology

A technology of microfluidic paper chips and tea polyphenols, which is applied in fluid controllers, chemical instruments and methods, and laboratory containers, etc. It can solve the problems of long detection time, slow color reaction speed, and large amount of reagents. , to achieve the effect of fast detection, low cost and simple operation

Active Publication Date: 2017-01-25
TEA RES INST CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The invention aims to solve the problems of slow folinol-tea polyphenol color reaction speed, large amount of reagents and ...

Method used

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  • Quick detection method of tea polyphenol contents on basis of micro-fluidic paper chip technology
  • Quick detection method of tea polyphenol contents on basis of micro-fluidic paper chip technology
  • Quick detection method of tea polyphenol contents on basis of micro-fluidic paper chip technology

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Experimental program
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Effect test

Embodiment 1

[0040] The schematic diagram of the structure of the microfluidic paper chip with symmetrical channel configuration is as follows: figure 1 as shown,

[0041] figure 1 In , the dotted box represents the edge of the chip; the black solid line represents the channel wall with hydrophobic properties, and the parts outside the black solid line and between the dotted box are all hydrophobic regions, surrounded by black to realize the symmetrical hydrophilic channel network in the middle. figure 1 Among them, the circular area where 1-8 are located is the reaction detection area; the strip area directly connected with each reaction detection area is the chip hydrophilic channel; each channel meets at the center of the chip, and the intersection point is the central area, indicated by the mark O; The distances from each detection area to point O are strictly equal. The length of the channel is 3 mm, the width is 1 mm, the diameter of the reaction detection area is 5 mm, and 8 hydro...

Embodiment 2

[0061] In order to check the reliability of the detection method of the present invention and the characteristics of the irrelevance of the detection result and the color reaction process, the inventor also investigated conditions such as the color reaction time during the invention, and the specific experiments are as follows:

[0062] Condition screening experiment 1: Prepare gallic acid aqueous solutions with concentrations of 0 μg / ml, 10 μg / ml, 25 μg / ml, 50 μg / ml, 75 μg / ml, 100 μg / ml, 150 μg / ml, and 200 μg / m, respectively, in the same chip with 8 channels In the chip, the signal scanning measurement of the above aqueous solution under different color reaction time conditions is carried out, respectively at 1min, 2min, 3min, 5min, 8min, 10min, 12min, 15min, 18min, 20min, 25min after the color reaction starts, Measure the gray value at 30min, 60min, and 90min and draw the working curve. The actual gray signal result after deducting the gray value of the blank detection area ...

Embodiment 3

[0065] The method established by the invention can not only be used to determine the aqueous solution of tea polyphenols, but also can realize rapid determination of the tea polyphenols solution containing organic solvents. In order to test this point, the inventor prepared the methanol extract of tea polyphenols according to the extraction method of tea polyphenols in tea leaves stipulated in the national standard of China (GB / T 8313-2008). After the solution was diluted 50 times, 20 μg / ml concentration of gallic acid standard, the recovery rate determination experiment.

[0066] According to the method of Example 1, the color development was carried out for 3 minutes, and the gallic acid additions measured by using 3 different chips were 21.6 μg / ml, 16.2 μg / ml and 19.4 μg / ml respectively, and the corresponding detection recovery values ​​were respectively 108%, 81% and 92%, showing that the method has good reliability.

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Abstract

The invention provides a quick detection method of tea polyphenol contents on the basis of a micro-fluidic paper chip technology. The quick detection method comprises the following steps: arranging a symmetrical hydrophilic channel network on a micro-fluidic paper chip, wherein the symmetrical hydrophilic channel network comprises a center area and a plurality of hydrophilic channels around the center area, one end of each hydrophilic channel is communicated with the center area, one end, far away from the center area, of each hydrophilic channel is provided with a reaction detection area, and the plurality of hydrophilic channels have the same length and width and form point symmetry; in each reaction detection area, dropwise adding a foline-phenol reagent and carrying out sample introduction, dropwise adding sodium carbonate solution in the center area, diffusing to each detection area through the hydrophilic channels, carrying out developing reaction for 1-10min, and carrying out semi-quantitative measurement or quantitative measurement of tea polyphenol. According to the method, a symmetrical multi-channel detection array of the micro-fluidic paper chip is used for carrying out parallel developing reaction and integral data acquisition, and a foline-phenol developing-detecting array with highly-consistent reaction time is obtained, so that accurate colorimetric determination can be realized at any time point in a developing reaction process, and time required for detection is greatly shortened.

Description

technical field [0001] The invention relates to a rapid detection method for tea polyphenol content based on microfluidic paper chip technology. Background technique [0002] Polyphenols have attracted extensive attention of researchers in recent years because of their potential health-promoting effects, and their total content is one of the important parameters in the quality inspection of many products (such as tea, red wine, cocoa, etc.). The spectrophotometric method based on the color reaction of folin phenol is the earliest developed method for detecting the total amount of polyphenols, and because of its advantages of simple equipment, convenient operation, and accurate measurement results, it is currently the most widely used food in the world. The determination method of the total amount of polyphenols is also the current national standard determination method of the total amount of polyphenols in tea polyphenol products and tea in my country. [0003] However, in v...

Claims

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Application Information

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IPC IPC(8): G01N21/78B01L3/00
CPCB01L3/5027B01L2300/161B01L2400/0406G01N21/78
Inventor 郝振霞鲁成银刘新
Owner TEA RES INST CHINESE ACAD OF AGRI SCI
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