The invention provides a quick detection method of tea
polyphenol contents on the basis of a micro-fluidic paper
chip technology. The quick detection method comprises the following steps: arranging a symmetrical hydrophilic
channel network on a micro-fluidic paper
chip, wherein the symmetrical hydrophilic
channel network comprises a center area and a plurality of hydrophilic channels around the center area, one end of each hydrophilic channel is communicated with the center area, one end, far away from the center area, of each hydrophilic channel is provided with a reaction detection area, and the plurality of hydrophilic channels have the same length and width and form
point symmetry; in each reaction detection area, dropwise adding a foline-
phenol reagent and carrying out sample introduction, dropwise adding
sodium carbonate solution in the center area, diffusing to each detection area through the hydrophilic channels, carrying out developing reaction for 1-10min, and carrying out semi-quantitative measurement or quantitative measurement of tea
polyphenol. According to the method, a symmetrical multi-channel detection array of the micro-fluidic paper
chip is used for carrying out parallel developing reaction and integral
data acquisition, and a foline-
phenol developing-detecting array with highly-consistent reaction time is obtained, so that accurate colorimetric determination can be realized at any time point in a developing reaction process, and time required for detection is greatly shortened.