The invention provides a substrate-free LED filament and a manufacturing method thereof, and a substrate-free LED filament lamp. The filament comprises at least a string of LED chips with same or different illuminant colors, electric lead-out wires, and electric connecting wires arranged between the chips and between the chips and the electric lead-out wires, welding terminals of the chips, the electric connecting wires, and the electric lead-out wires are coated by first luminescent powder layers to form an initial substrate-free LED filament, the other side of the initial substrate-free LED filament is coated by a second luminescent powder layer to form the substrate-free LED filament, and the substrate-free LED filament can be adhered on the external surface of a transparent high-heat-conductivity tube via transparent adhesive tapes or luminescent powder glue to form a cylindrical substrate-free LED filament light source used for manufacturing a high-power LED filament lamp. The substrate-free LED filament lamp comprises at least a bulb shell sealed in a vacuum manner and inflated with heat radiation protection gas, at least one substrate-free LED filament light source is arranged in each bulb shell, and the substrate-free LED filament light source comprises the initial substrate-free LED filament, the substrate-free LED filament or the cylindrical substrate-free LED filament light source; an LED drive, a drive shell, and an electric connector etc. and used for illumination.