Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Colloidal gold semi-quantitative quick immunity diagnosis test-paper stripe

A technology of rapid immunization and test strips, applied in the direction of biological testing, material inspection products, etc., to achieve simple detection procedures, accurate and reliable results, and simple production

Inactive Publication Date: 2007-01-10
LANZHOU UNIVERSITY
View PDF0 Cites 20 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The support is made of equal width PVC plastic board

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Colloidal gold semi-quantitative quick immunity diagnosis test-paper stripe

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment 2

[0009] (1) Purchasing type A and III influenza virus-specific antigen neuraminidase (NA) and hemagglutinin protein to immunize mice, and reassured to obtain mouse-derived specific monoclonal antibodies IgG1 and IgG2 against the two antigens. (2) IgG1 was selected as the gold-labeled antibody, and the sodium citrate reduction method was used to prepare and determine that the optimal size of labeled gold nanoparticles was 15 nm, and centrifuged at 7300 rpm / min for 20 minutes to prepare a stock solution. 15nm colloidal gold and labeled monoclonal antibody were used to determine the optimal amount of protein, and the pH9.0 borate buffer was used to make the labeled monoclonal antibody a gradient of 5-50Ug / ml. After adding the gold stock solution, 10% NaCl was added for stability experiments. Minutes later, centrifuge and measure OD580nm, and the protein concentration is 10ug / ml when the optical density is stable. After preparation, add PEG to make colloidal gold stable solution. ...

specific Embodiment 3

[0011] (1) Purchasing pure staphylococcal protein A (SPA) to determine the protein content (2) Using the sodium citrate reduction method to determine the size of the optimally labeled gold nanoparticle for the SPA used, centrifuged at 2000rpm / min for 30 minutes to prepare a stock solution . Use 40nm colloidal gold and SPA to determine the optimal amount of protein, use PH9.0 borate buffer to make the labeled SPA a gradient of 5-50Ug / ml, add 10% NaCl after adding gold stock solution for stability experiment, and centrifuge for 5 minutes The OD580nm was measured, and the SPA protein concentration was 4.6ug / ml when the optical density was stable. After preparation, add PEG to make colloidal gold stable solution. (3) Absorb the marked gold standard SPA with a sterile absorbent sponge with a width of 1 cm, a width of 1.5 cm, and a thickness of 0.08 cm to make a gold marker pad. In a dark environment at 4°C, blow dry slowly with nitrogen gas for use. (4) Select sterile cellulose a...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention relates to a colloidal gold semiquantitative immunologic diagnosis test paper preparing and assembly method. Said test paper utilizes immunity chromatograph principle capable of rapid visual inspection nano colloidal gold marker and sample combined colour, judging detective semiquantitative result through test zone with parallel reference zone colour contrast.

Description

[0001] Technical field The present invention relates to a kind of nano-colloidal gold test paper for rapid semi-quantitative detection of pathogens (antibodies or antigens), and also relates to the design and assembly organization of the test paper. Background technique [0002] Existing test strips for immunodiagnosis are the most convenient and fast among immunodiagnostic reagents. According to diagnostic categories, they can be divided into infectious diseases, endocrine, tumors, drug testing, etc. However, the current test strips are mainly based on the qualitative detection of colloidal gold from the perspective of the methodology of result judgment. The test results can only determine the presence or absence of pathogens but cannot determine whether their content is higher than a certain diagnostic standard. In practical applications, judging the content of a certain substance to be detected is of more reference value for clinical diagnosis. Qualitative colloidal gold im...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53G01N33/52
Inventor 李红玉张波
Owner LANZHOU UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com