Semi-quantitative detecting test paper of cardiac troponin and preparation method thereof

Abstract

The invention relates to semi-quantitative detecting test paper of cardiac troponin, mainly comprising five parts: a sample pad, a combination pad, a water sucking pad, a soleplate and a nitric acid fibre film, wherein two parallel antibody capture lines are made on the nitric acid fibre film; one first anti-cardiac troponin I monoclonal antibody with the determined concentration close to the combination pad is used as a detecting line; another goat-anti-rabbit IgG antibody with the determined concentration is used as a standard concentration developing contrast line; the distance between the two lines is 4-7 mm; and the combination pad is sprayed with two substances of a second anti-cardiac troponin I monoclonal antibody and a gold rabbit IgG marked by colloidal gold or colour latex. The quantitative detecting test paper of cardiac troponin in the invention has the advantages of simple preparation method, low cost, convenience for detection and use, and capability of rapidly conveniently semi-quantitatively detecting the content of the cardiac troponin I in the human body blood sample, and is suitable for family application and popularization.

Description

technical field [0001] The invention relates to a test paper for semi-quantitative detection of cardiac troponin. Background technique [0002] Cardiovascular and cerebrovascular diseases are the number one killer that endangers human life and health, and the incidence trend is also increasing year by year. There are 450 million acute myocardial infarction (AMI) patients in the emergency department of the United States, and 400 million patients have been diagnosed with acute myocardial infarction, of which 2 -3% of people really have AMI, and due to the diversity of symptoms of AMI, there are considerable misdiagnosis and missed diagnosis of complications hidden in the clinic. Some myocardial injuries are ignored by doctors and patients because they are not typical, and they are not treated in time. There are many dangers to life. The onset of acute myocardial infarction is acute and the mortality rate is high. Early diagnosis and timely treatment of AMI are very important ...

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to overcome the deficiency that the current colloidal gold immunochromatography test strips can only be qualitatively detected but not quantitatively detected in the actual popularization and use, and provide a kind of specific instrument and professional equipment that can quickly evaluate the detection results , and conduct a semi-quantitative determination on the test results, and provide the preparation method of the test paper at the same time

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