Preparation of carbamide detection test paper for dairy food and carbamide detection method

A technology for detecting test paper and urea, which is applied in the direction of material analysis by observing the influence of chemical indicators, and analysis by making materials undergo chemical reactions, can solve the problems that there is no simple, fast and portable method for urea, and achieve The detection method is simple and fast, the cost is reduced, and the effect of simplifying the detection procedure

Inactive Publication Date: 2009-04-01
XI AN JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] It can be seen that there is no simple, fast and portable method suitable for urea detection at present

Method used

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  • Preparation of carbamide detection test paper for dairy food and carbamide detection method
  • Preparation of carbamide detection test paper for dairy food and carbamide detection method
  • Preparation of carbamide detection test paper for dairy food and carbamide detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Example 1: Preparation of concanavalin-phenol red test paper and its detection of urea-adulterated milk

[0067] 1. Preparation of urea test paper:

[0068] 1) Preparation of indicator solution: prepare a saturated solution of phenol red or its sodium salt with deionized water for later use;

[0069] 2) Preparation of test paper stock solution: take 8g of sword beans, grind them into powder, dissolve them in 100ml of deionized water, filter through 6 layers of gauze to remove residue, and set the filtrate for use;

[0070] 3) Mix equal volumes of the solutions obtained in steps 1) and 2) to form a test paper stock solution;

[0071] 4) Immerse the quantitative filter paper in the original solution of the test paper to fully absorb and saturate it, drain off the excess liquid, spread it on a glass plate and let it dry naturally to form the urea test paper.

[0072] 5) Cut the urea test paper into small squares and use it directly or connect it to the handle.

[0073] ...

Embodiment 2

[0076] Example 2: Preparation of soybean flour-phenol red test paper and its detection of urea-adulterated milk

[0077] 1. Preparation of urea test paper:

[0078] 1) Preparation of indicator solution: prepare a saturated solution of phenol red or its sodium salt with distilled water, dilute to 50%, and set aside;

[0079] 2) Preparation of test paper stock solution: take 5 g of soybeans and grind them into powder, add 100 ml of the indicator solution with a saturation degree of 50% prepared in step 1), mix and settle naturally for 10-30 minutes, and take the upper suspension to be the test paper stock solution;

[0080] 3) Dip the stock solution of the test paper with a large brush and evenly paint it on the quantitative filter paper spread on the glass plate, so that the test paper is fully penetrated into the solution and dried naturally to form the urea test paper;

[0081]4) Cut the urea test paper into small pieces and use it directly or connect it to the handle.

[0...

Embodiment 3

[0084] Example 3: Preparation of kidney bean flour-phenol red test paper and its detection of urea-adulterated milk

[0085] As in Example 1, the sword bean was replaced by kidney beans, and the urea detection test paper was prepared in the same way; and the prepared kidney bean powder-phenol red test paper was used to detect adulterated milk with different concentrations of urea, and the color development results were as follows: image 3 .

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Abstract

The invention provides a preparation method of urea detection test paper in dairy and a detection method of urea; urease source powder is taken and mixed with pH indicator with the pKHIn more than 6.8; the mixture is diluted by deioned water and the filter solution or the upper suspension liquid after natural deposition is taken, uniformly fixed on a carrier with good water absorption by the forms of coating, printing or soaking, and dried subsequently, thus forming the urea detection test paper; the detection method of the dairy comprises the steps as follows: firstly, common pH test paper is used for detecting the dairy to be detected; whether the dairy to be detected is in normal pH range 6.3-6.8 or not is checked; if so, the urea detection is directly carried out; if not, the pH value is adjusted to the normal pH range and the urea detection is carried out subsequently; one piece of urea detection test paper dips the dairy to be detected and the color change of the test paper is observed; if the color of the test paper is not changed, the dairy is qualified; if the color of the test paper changes, the dairy is mixed with the urea, thus carrying out qualitative judgment on the dairy; and the displayed color is compared with a standard colorimetric card, so as to carry out semi-quantitative detection of the urea in the dairy.

Description

technical field [0001] The invention relates to rapid qualitative or semi-quantitative detection of urea in food safety monitoring, in particular to a qualitative or semi-quantitative determination method for urea in urea-adulterated milk and a preparation method for the urea detection test paper used therein. Background technique [0002] Urea detection is a common indicator of laboratory testing, used for food industry, feed industry safety supervision monitoring and anti-counterfeiting and environmental protection testing, such as detection of urea-adulterated fresh milk, adulteration of animal protein materials, urea content in swimming pool water detection and so on. [0003] At present, there are many laboratory methods for urea detection. Most of the rapid determination of urea in milk uses the colorimetric method. The chromogenic reagents used mainly include p-dimethylaminobenzaldehyde, nitrite-Gries reagent and diacetyl Oxime, etc., these methods require the use of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
Inventor 杨水云马晓明徐凡谭丹来宝长
Owner XI AN JIAOTONG UNIV
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