Colloidal gold immunochromatography test paper for rapidly diagnosing brucellosis

A technology for immunochromatographic detection and brucellosis, which is applied in the field of immunodiagnosis, can solve problems such as inaccurate detection results, achieve direct detection results, short detection time, and avoid detection errors

Pending Publication Date: 2020-11-03
HANGZHOU ALLTEST BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The present invention aims to overcome the problem that the antigen detection of Brucella antibodies in the prior art will lead to inaccurate detection results, and provides a colloidal gold immunochromatographic detection test paper for rapid diagnosis of Brucellosis, which can be accurately detected. Whet

Method used

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  • Colloidal gold immunochromatography test paper for rapidly diagnosing brucellosis
  • Colloidal gold immunochromatography test paper for rapidly diagnosing brucellosis
  • Colloidal gold immunochromatography test paper for rapidly diagnosing brucellosis

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Effect test

Embodiment 1

[0046] The preparation method of the colloidal gold immunochromatography detection test paper of described quick diagnosis brucellosis, specifically comprises the following steps:

[0047] 1) Preparation of test line T and quality control line C: Dilute mouse anti-Brucella antibody and goat anti-mouse IgG antibody solutions and spray them on nitrocellulose membrane at a speed of 1.0ul / cm respectively as test lines T and the quality control line C are placed in 36 ℃ and dried to obtain the detection line and the quality control line; the coating of the detection line T is coated with a mouse anti-brucella antibody concentration of 0.1 mg / ml; the quality control line is 0.1mg / ml; The concentration of goat anti-mouse IgG antibody coated with control line C is 1.0mg / ml;

[0048] 2) Preparation of the gold-labeled pad: Dilute the purified gold-labeled antibody to a concentration of OD1 and coat it on a glass fiber pad and place it in a freeze dryer to obtain a gold-labeled pad; the...

Embodiment 2

[0051] The preparation method of the colloidal gold immunochromatography detection test paper of described quick diagnosis brucellosis, specifically comprises the following steps:

[0052] 1) Preparation of test line T and quality control line C: Dilute mouse anti-Brucella antibody and goat anti-mouse IgG antibody solutions and spray them on nitrocellulose membrane at a speed of 1.05ul / cm respectively as test lines T and the quality control line C are placed in 36.5 ℃ and dried to obtain the detection line and the quality control line; the coating of the detection line T is coated with a mouse anti-brucella antibody concentration of 0.3mg / ml; the quality control line is 0.3mg / ml; The concentration of goat anti-mouse IgG antibody coated with control line C is 1.5mg / ml;

[0053] 2) Preparation of the gold-labeled pad: Dilute the purified gold-labeled antibody to a concentration of OD2 and coat it on a glass fiber pad and place it in a freeze dryer to obtain a gold-labeled pad; t...

Embodiment 3

[0055] The preparation method of the colloidal gold immunochromatography detection test paper of described quick diagnosis brucellosis, specifically comprises the following steps:

[0056] 1) Preparation of test line T and quality control line C: Dilute mouse anti-Brucella antibody and goat anti-mouse IgG antibody solutions respectively, and spray them on nitrocellulose membrane at a speed of 1.1ul / cm as test lines respectively T and the quality control line C are placed in 37 ℃ and dried to obtain the detection line and the quality control line; the coating of the detection line T is coated with a mouse anti-brucella antibody concentration of 0.5mg / ml; the quality control line is 0.5mg / ml; The concentration of goat anti-mouse IgG antibody coated with control line C is 2.0mg / ml;

[0057] 2) Preparation of the gold-labeled pad: Dilute the purified gold-labeled antibody to a concentration of OD2 and coat it on a glass fiber pad and place it in a freeze dryer to obtain a gold-lab...

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Abstract

The invention relates to the technical field of immunodiagnosis, and discloses colloidal gold immunochromatography detection test paper for rapidly diagnosing brucellosis and a preparation method of the colloidal gold immunochromatography detection test paper for rapidly diagnosing brucellosis in order to solve the problem of inaccurate detection results caused by antigen detection of brucellosisantibodies in the prior art. The method specifically comprises the following steps: 1) preparing a detection line T and a quality control line C; 2) preparing a gold-labeled pad; 3) preparing the testpaper: sequentially connecting and assembling the water absorption pad, the sample pad, the microsphere probe treatment pad, the antibody-coated modified nitrocellulose membrane and the PVC bottom plate to obtain a finished product. Whether brucellosis exists or not can be accurately detected, the product adopts an antibody antigen detection mode, and the antigen has no incubation period; compared with a product for detecting an antigen, the test paper is more accurate, the antigen detection accuracy is higher than that of a product for detecting the antibody, the detection efficiency is high, and the preparation process is simple.

Description

technical field [0001] The invention relates to the technical field of immunodiagnosis, in particular to a colloidal gold immunochromatographic detection test paper for rapidly diagnosing brucellosis. Background technique [0002] Brucellosis, referred to as brucellosis for short, is a zoonotic infectious allergic disease with Brucella. Brucellosis is highly contagious and has been classified as a Category B pathogen by the CDC and is therefore considered a potential biological weapon. The disease exists in more than 170 countries and regions in the world. The prevalence of brucellosis among humans and animals threatens 1 / 5-1 / 6 of the world's population. There are about 5-6 million people suffering from brucellosis worldwide, and the annual economic losses are as high as billions of dollars. And the disease also poses a serious threat to human health. [0003] Human Brucella infection is due to contact with sick animals on the one hand, and contact or consumption of vario...

Claims

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Application Information

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IPC IPC(8): G01N33/569G01N33/558
CPCG01N33/56911G01N33/558G01N2333/23G01N2469/10
Inventor 陈金树张芳陆维克高飞
Owner HANGZHOU ALLTEST BIOTECH
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