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Group A streptococcus antigen detection test strip and kit and preparation methods thereof

An antigen test strip and antigen detection technology, applied in the field of group A streptococcal antigen detection card and its preparation, can solve the problems of lack of group A streptococcus kits, etc., and achieve the goals of reducing interference from other pathogens, good specificity, and eliminating false positives Effect

Inactive Publication Date: 2019-12-13
JIANGSU BIOPERFECTUS TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is a lack of kits for the detection of group A streptococci on the market.

Method used

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  • Group A streptococcus antigen detection test strip and kit and preparation methods thereof
  • Group A streptococcus antigen detection test strip and kit and preparation methods thereof
  • Group A streptococcus antigen detection test strip and kit and preparation methods thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] The preparation method of the group A streptococcal antigen detection card provided in this embodiment, the specific steps are as follows:

[0050] The colloidal gold solution used in this example was self-produced; the anti-group A streptococcus antibody and the goat anti-mouse IgG polyclonal antibody were purchased from Shanghai Shuoying Biotechnology Co., Ltd.; the gold spraying device was purchased from Shanghai Jiening Biotechnology Co., Ltd.

[0051] (1) Colloidal gold-labeled anti-group A streptococcus antibody

[0052] Use 300uL of 0.2M potassium carbonate solution to adjust the pH value in 50ml of 0.01% colloidal gold solution; under stirring conditions, slowly add 0.5ml of 1mg / ml anti-group A streptococcus antibody to be labeled into the above In the colloidal gold solution, mix well, and let stand at room temperature for 30 minutes;

[0053] The mixed solution was centrifuged at 8000g, 4°C for 30min, the supernatant was removed, and the loose precipitate was...

Embodiment 2

[0080] Example 2 kit assembly

[0081] Take the aluminum foil bag and desiccant; open the heat sealer and preheat; put the test card and 1 bag of desiccant prepared in Example 1 to be bagged into the aluminum foil bag; seal the aluminum foil bag with a heat sealer; Label.

[0082] The aluminum foil bag with the detection card, the sampling straw, and the instruction box are the finished product of the group A streptococcal antigen detection kit.

Embodiment 3

[0084] The method for detecting samples using the Group A Streptococcus antigen detection card provided in Example 1 or the Group A Streptococcus antigen detection kit provided in Example 2:

[0085] When using the group A streptococcal antigen detection card to test, drop about 100ul of the sample to be tested into the sample hole and place it horizontally.

[0086] If the sample contains group A streptococcal antigen, the anti-group A streptococcal antibody-colloidal gold combines with the group A streptococcal antigen in the sample to form an "antigen-antibody-gold" complex, and the complex moves along the test strip Chromatography moves, and first reaches the detection line coated with anti-group A streptococcus antibody, and the "antigen-antibody-gold" complex will combine with anti-group A streptococcus antibody and stay on the detection line to form a red line line, which indicates a positive result. The shade of the line color is not proportional to the amount of anti...

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Abstract

The invention discloses a group A streptococcus antigen detection test strip and kit and preparation methods thereof, and relates to the technical field of group A streptococcus detection. The group Astreptococcus antigen test strip is prepared by sequentially attaching a nitrocellulose membrane coated with an anti-group A streptococcus detection antibody, an immune binding pad coated with an anti-group A streptococcus immunolabeled antibody, absorbent paper and a sample pad to a polyvinyl chloride bottom plate; a detection card can detect whether a group A streptococcus antigen is present ina sample to be detected or not by detecting a marker; and the test strip and the detection card comprising the test strip can specifically and quickly detect and diagnose early infection of group A streptococcus, thereby reducing the cost, realizing rapid detection and meeting the requirements of clinical use.

Description

Technical field: [0001] The invention relates to the technical field of group A streptococcus detection, in particular to a group A streptococcus antigen detection card, a preparation method thereof and a detection kit. Background technique: [0002] Group A Streptococcus (Strep-A), also known as Streptococcus pyogenes, is an important human pathogenic bacteria worldwide. It can not only cause infection alone, but also cause mixed infection with other pyogenic bacteria. If patients, especially children, do not receive anti-infection treatment in time, it is easy to cause acute pharyngitis, impetigo, toxic shock syndrome, invasive fasciitis, scarlet fever, pneumonia, erysipelas and allergic diseases, such as acute rheumatic fever (ARF) , rheumatic heart disease (RHD), acute glomerulonephritis, etc. [0003] It is estimated that 517,000 people die from severe Strep-A infection and disease every year in the world, and another 18.1 million suffer from severe Strep-A infection,...

Claims

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Application Information

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IPC IPC(8): G01N33/558G01N33/569
CPCG01N33/558G01N33/56944
Inventor 杨标金巍刘中华王国强
Owner JIANGSU BIOPERFECTUS TECH CO LTD
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