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A novel coronavirus antigen detection test strip
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A coronavirus, antigen detection technology, applied in the field of immunochromatography
Active Publication Date: 2021-09-24
NANTONG EGENS BIOTECH CO LTD
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Also, assisted care for infected people can be very effective
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Embodiment 1
[0029] A novel coronavirus antigen detection test strip, such as figure 1 As shown, a sample pad 2, a colloidal gold adsorption pad 3, a blood filtration membrane 7, an antibody carrying membrane 4, and a water-absorbent pad 5 are overlapped successively on the substrate 1; A protective film 6 is provided;
[0030] The antibody carrying membrane 4 is provided with a detection line T and a quality control line C spaced apart, the detection line T is close to the colloidal gold adsorption pad, and the quality control line C is close to the water absorption pad;
[0031] The colloidal gold adsorption pad 3 is coated with colloidal gold-labeled novel coronavirus monoclonal antibody B and colloidal gold-labeled mouse IgG antibody;
[0032] The detection line T is coated with novel coronavirus monoclonal antibody A;
[0033] The quality control line C is coated with anti-mouse IgG polyclonal antibody.
Embodiment 2
[0035] This embodiment provides the preparation method of the novel coronavirus antigen detection test strip of embodiment 1, comprising the following steps:
[0036] 1. Preparation of antibody-carrying membrane:
[0037] (1) Select a nitrocellulose membrane with a pore size of 3 μm to 10 μm, cut the membrane into specifications with a width of 2.0 cm and a length of 30.5 cm as required, and set aside.
[0038] (2) Use 0.1M, pH8.0 Tris-HCL buffer to prepare the novel coronavirus monoclonal antibody A used for the detection line coating, the antibody concentration is 1.2~1.5mg / ml, in this embodiment, it is 1.3mg / ml ml; prepare the anti-mouse IgG polyclonal antibody used for quality control line coating with 0.85% sodiumchloridebuffer solution by mass percentage, so that the antibody concentration is (1.5~1.8) mg / ml, which is 1.6 in the present embodiment mg / ml.
[0039] (3) Select the antibody-coated surface of the nitrocellulose membrane and mark it, and coat the antibody ...
Embodiment 3
[0053] This embodiment provides a novel coronavirus antigen detection kit, including the novel coronavirus antigen detection test strip described in Example 1.
[0054] Further, it also includes a lysate, which includes the following components: Tris-HCl 1.6-2.4mol / L, SDS mass percentage 0.8%-1.2%, polyvinylpyrrolidonemass percentage 0.4%-0.6%, pH8 .0-9.0.
[0055] Further, the lysate includes the following components: Tris-HCl 2mol / L, SDS 1% by mass, polyvinylpyrrolidone 0.5% by mass, pH 8-9.
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Abstract
A novel coronavirusantigen detection test strip provided by the present invention comprises: a substrate, and a sample pad, a colloidal gold adsorption pad, a blood filter membrane, an antibody-carrying membrane and a water-absorbent pad are sequentially overlapped and overlapped on the substrate; The antibody carrying membrane is provided with a detection line and a quality control line at intervals, the detection line is close to the colloidal gold adsorption pad, and the quality control line is close to the water absorption pad; the coating of the colloidal gold adsorption pad Colloidal gold-labeled novel coronavirusmonoclonalantibody B and colloidal gold-labeled mouse IgG antibody; the detection line is coated with novel coronavirusmonoclonal antibody A; the quality control line is coated with anti-mouse IgG polyclonal antibody; novel coronavirus The virusmonoclonal antibody B is different from the new coronavirus monoclonal antibody A; by setting a blood filter membrane, it can prevent the sample to be tested from being viscous and not running, and can also filter out other macromolecular interfering substances to improve specificity.
Description
technical field [0001] The invention relates to the technical field of immunochromatography, in particular to a novel coronavirus antigen detection test strip. Background technique [0002] 2019 Novel Coronavirus, on January 12, 2020, the World Health Organization officially named it 2019-nCoV. Coronaviruses are a large family of viruses known to cause colds as well as more serious illnesses such as Middle East Respiratory Syndrome (MERS) and Severe Acute Respiratory Syndrome (SARS). The novel coronavirus is a new strain of coronavirus that has never been found in humans before. [0003] Common signs after a person is infected with the new coronavirus include respiratory symptoms, fever, cough, shortness of breath, and dyspnea. In more severe cases, infection can lead to pneumonia, severe acute respiratory syndrome, kidney failure, and even death. Many symptoms are manageable, so treatment should be tailored to the patient's clinical condition. Also, assisted care for in...
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