Multi-item respiratory tract antigen detection card and kit

Abstract

The invention relates to a multi-item respiratory tract antigen detection card which comprises an influenza A virus antigen test strip, an influenza B virus antigen test strip, a respiratory tract adenovirus antigen test strip, a respiratory tract syncytial virus antigen test strip and a mycoplasma pneumoniae antigen test strip, wherein each of the influenza A virus antigen test strip, the influenza B virus antigen test strip, the respiratory tract adenovirus antigen test strip, the respiratory tract syncytial virus antigen test strip and the mycoplasma pneumoniae antigen test strip comprisesa colloidal gold conjugate pad, and each colloidal gold conjugate pad comprises a streptavidin conjugate pad and a double-nanoparticle double-labeled antibody conjugate pad; the test strip in the detection card enables biological raw materials to react fully, improves the sensitivity of antigen detection, effectively reduces missing detection, and meanwhile, the blocking agent is added into the sample pad to improve the specificity, so that influenza A virus, influenza B virus, respiratory adenovirus, respiratory syncytial virus and mycoplasma pneumoniae antigen can be detected at the same time.

Description

technical field [0001] The invention relates to the field of human respiratory virus detection, in particular to an antigen detection card and kit for influenza A virus, influenza B virus, respiratory adenovirus, respiratory syncytial virus and mycoplasma pneumoniae. Background technique [0002] There are three types of influenza virus, A, B, and C. Type A is the most likely to cause epidemics, followed by Type B, and Type C rarely causes epidemics. According to the antigenicity of outer membrane phytohemagglutinin (HA) and neuraminidase (NA) proteins, influenza A virus can be divided into 15 H subtypes (H1-H15) and 9 N subtypes (N1-H15). ~N9). Because the nucleic acid sequences encoding HA and (or) NA are prone to mutations, resulting in changes in the antigenic epitopes of HA and (or) NA, this antigenic change makes the original specific immunity of the population invalid, so influenza A Viruses often cause large-scale or even worldwide influenza epidemics. Most suscep...

AI Technical Summary

Problems solved by technology

Isolation and culture have diagnostic significance, but the rate of missed diagnosis is high. Therefore, serotype detection is mostly used for diagnosis in the later stage. Antigen detection, with its advantages of high sensitivity and specificity, gradually replaces serological detection and plays an important role in the prevention of pathogen infection. , diagnosis, treatment, and prognosis