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Method for improving sensitivity of latex enhanced turbidimetric immunoassay

A latex-enhanced, immune-turbidimetric technology, used in instruments, color/spectral property measurement, measurement devices, etc., can solve the problems of increasing reagent costs and increasing costs, and achieve the effect of increasing costs and reducing costs.

Active Publication Date: 2015-02-18
ZYBIO INC
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0004] With the development of laboratory medicine, more and more low-concentration detection substances are detected, and the concentration of detection substances is decreasing from the μg / mL level to the ng / mL level. The sensitivity requirements for diagnostic reagents are also getting higher and higher. For traditional For the latex-enhanced immunoturbidimetric method, it is necessary to further improve the detection sensitivity to meet the detection requirements. The traditional methods to improve the detection sensitivity include using antibodies with higher affinity, using larger particles, and latex particles with better stability. Choose a better buffer system to promote the antigen-antibody reaction, but these will increase the cost of the reagent. How to improve the analytical sensitivity and precision of the analyte at the ng / mL level without increasing the cost has always been a part of reagent development. big challenge

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  • Method for improving sensitivity of latex enhanced turbidimetric immunoassay
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  • Method for improving sensitivity of latex enhanced turbidimetric immunoassay

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Embodiment 1

[0039] 1. Preparation of Quantitative Detection Latex Enhanced Immunoturbidimetric Kit

[0040] Preparation of Reagent 1:

[0041] Prepared from 50mM Tris-HCl buffer, 3g bovine serum albumin BSA, 1g sodium azide, 2.2g stachyose, 0.6g alum, 1.9g sodium fructose diphosphate, 0.3g sodium hexametaphosphate and 50g PEG6000 Obtain, the concentration of Tris-HCl buffer solution in the final reagent 1 is 50mM, the concentration of bovine serum albumin BSA is 3g / L, the concentration of sodium azide is 1g / L, the concentration of PEG6000 is 50g / L, stachyose 2.2 g / L, alum 0.6g / L, sodium fructose diphosphate 1.9g / L, sodium hexametaphosphate 0.3g / L;

[0042] Weigh 6.06g Tris (Tris), 3g BSA, 50g PEG6000, 2.2g stachyose, 0.6g alum, 1.9g sodium fructose diphosphate, 0.3g sodium hexametaphosphate, 1g sodium azide and dissolve in 0.8L of deionized water, adjust the pH to 7.0 with HCl, and dilute to 1L to obtain reagent 1.

[0043] Preparation of Reagent 2:

[0044] Prepared from 50mM Tris-HC...

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Abstract

The invention relates to the field of in-vitro diagnosing reagents, and particularly relates to a method for improving the sensitivity of latex enhanced turbidimetric immunoassay. The method comprises the following steps: (1) selecting latex particles with large diameters to react with an antibody, and carrying out antibody coating; (2) washing and sealing the coated latex, dispersing the latex in a buffer solution to prepare a reagent 2 of a latex enhanced turbidimetric immunoassay kit; (3) preparing a reagent 1 of the latex enhanced turbidimetric immunoassay kit; (4) setting parameters on a full-automatic biochemical analyzer; (5) operating the biochemical analyzer to calibrate a sample with known concentration and detect an absorbance change value of the sample to be detected, and calculating the content of to-be-detected object in the sample according to the calibration curve. The method for improving the sensitivity of latex enhanced turbidimetric immunoassay is capable of greatly improving the analyzing sensitivity and precision of a reagent without increasing the detection cost, and has an excellent application prospect.

Description

technical field [0001] The invention relates to the field of in vitro diagnostic reagents, in particular to a method for improving the sensitivity of latex-enhanced immune turbidimetry. Background technique [0002] When light passes through a turbid medium solution, due to the presence of turbid particles in the solution, part of the light is absorbed, and the amount absorbed is proportional to the amount of turbid particles. This method of measuring light absorption is called turbidimetry. This method was earlier reported by Schultre and Schuick in 1959 and applied to the formation of complexes after the binding of plasma proteins and antibodies, resulting in changes in turbidity, and then the transmission turbidimetry. Generally, the transmission turbidimetry of antibody to antigen quantification is used, called For immunoturbidimetry. The principle is to use the specific combination of antigen and antibody to form a complex, and to quantify the antigen or antibody by me...

Claims

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Application Information

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IPC IPC(8): G01N33/546G01N21/31
CPCG01N33/546
Inventor 刘霖
Owner ZYBIO INC
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