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Kit and method for detecting concentration of Kappa free light chains

A technology of free light chains and kits, applied in the biological field, can solve the problems of non-linearity in the determination of Kappa free light chains, and achieve the effects of facilitating production amplification and popularization, shortening detection time, and simple and effective methods

Active Publication Date: 2014-04-16
BYRON DIAGNOSTICS SHANGHAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide a test kit and method for measuring the concentration of Kappa free light chain in order to overcome the non-linear defect of existing Kappa free light chain assay

Method used

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  • Kit and method for detecting concentration of Kappa free light chains
  • Kit and method for detecting concentration of Kappa free light chains
  • Kit and method for detecting concentration of Kappa free light chains

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Improve the A kit, change the concentration of sodium chloride in the reaction buffer (R1 reagent) to 1250mmol / L, keep the concentration of other components unchanged, and record it as A-a kit, the specific composition is as follows:

[0050] Composition of the reaction buffer (R1 reagent) in the A-a kit

[0051] Element

name

concentration

1

4-Hydroxyethylpiperazineethanesulfonic acid (Hepes)

100mmol / L

2

Sodium chloride (NaCl)

1250mmol / L

3

Tween-20

1ml / L

4

Preservative Proclin-300

0.3ml / L

[0052] Composition of Kappa free light chain antibody solution (R2 reagent) in A-a kit

[0053] Element

name

concentration

1

Kappa Free Light Chain Antibody

0.5mg / ml

2

latex microspheres

3mg / ml

3

Tween-20

1ml / L

4

Preservative Proclin-300

0.3ml / L

[0054] Drawing of working curve:

[0055] Using the latex-enhanced t...

Embodiment 2

[0064] Improve the A kit, change the concentration of sodium chloride in the reaction buffer (R1 reagent) to 2000mmol / L, keep the concentration of other components unchanged, and record it as A-b kit, the specific composition is as follows:

[0065] Composition of reaction buffer (R1 reagent) in A-b kit

[0066] Element

name

concentration

1

4-Hydroxyethylpiperazineethanesulfonic acid (Hepes)

100mmol / L

2

Sodium chloride (NaCl)

2000mmol / L

3

Tween-20

1ml / L

4

Preservative Proclin-300

0.3ml / L

[0067] Composition of Kappa free light chain antibody solution (R2 reagent) in A-b kit

[0068] Element

name

concentration

1

Kappa Free Light Chain Antibody

0.5mg / ml

2

latex microspheres

3mg / ml

3

Tween-20

1ml / L

4

Preservative Proclin-300

0.3ml / L

[0069] Drawing of working curve:

[0070] Using the latex-enhanced turbidime...

Embodiment 3

[0080]Improve the B kit, change the concentration of sodium chloride in the reaction buffer (R1 reagent) to 1500mmol / L, keep the concentration of other components unchanged, and record it as B-a kit, the specific composition is as follows:

[0081] Composition of the reaction buffer (R1 reagent) in the B-a kit

[0082] Element

name

concentration

1

4-Hydroxyethylpiperazineethanesulfonic acid (Hepes)

100mmol / L

2

Sodium chloride (NaCl)

1500mmol / L

3

Tween-20

1ml / L

4

Preservative Proclin-300

0.3ml / L

[0083] Composition of Kappa free light chain antibody solution (R2 reagent) in B-a kit

[0084] Element

name

concentration

1

Kappa Free Light Chain Antibody

0.5mg / ml

2

latex microspheres

3mg / ml

3

Tween-20

1ml / L

4

Preservative Proclin-300

0.3ml / L

[0085] Drawing of working curve:

[0086] Using the latex-enhanced tu...

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Abstract

The invention discloses a kit and a method for detecting the concentration of Kappa free light chains. The kit comprises a reaction buffer solution and a Kappa free light chain antibody solution, wherein the concentration of sodium chloride in the reaction buffer solution is 1250-2400 mmol / L. According to the kit, the problem of poor clinical specimen linearity in a turbidimetry assay of the Kappa free light chains is solved, reliable Kappa free light chain detection results as diagnostic references are provided for clinical doctors, the advantages of high speed and flux of a latex-enhanced turbidimetric immunoassay can be conveniently brought into full play, the detection time is shortened, and the clinical application and patient diagnosis are facilitated.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a kit and a method for measuring the concentration of Kappa free light chains. Background technique [0002] Immunoglobulin (Ig) light chains are divided into two types, kappa (kappa) and lambda (lambda), each Ig molecule has only one type of light chain, and the ratio of human kappa (kappa) and lambda (lambda) is 6:4. Light chains are small molecular proteins that can freely pass through the glomerular basement membrane, and are reabsorbed back into the blood circulation in the renal tubules, so only a small amount of light chains exist in normal human urine. When metabolic disorders and multiple myeloma occur in the human body, a large number of free light chains appear in the blood and are excreted in the urine, which is Bence-Jones protein (protein of the week). In a normal human body, the serum κ-type free light chain is 3-19 mg / L, the λ-type free light chain is 6-26 mg / L, and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/531
CPCG01N21/31G01N33/52G01N33/6803G01N33/6893G01N2800/70
Inventor 王金凤蒋欣
Owner BYRON DIAGNOSTICS SHANGHAI
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