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Latex-enhanced turbidimetric immunoassay kit for quantitatively detecting adiponectin ADPN and preparation and application methods thereof

An immunoturbidimetric and quantitative detection technology, used in biological testing, measuring devices, material inspection products, etc., can solve the problems of limited detection sensitivity and cannot meet clinical applications, and achieve improved detection sensitivity, flexible and rapid quantitative detection, and high sensitivity. Effect

Pending Publication Date: 2020-06-05
ANHUI DAQIAN BIO ENG LIMITED
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the detection sensitivity of the existing latex-enhanced immunoturbidimetric assay kits is limited, which still cannot meet the needs of clinical applications.

Method used

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  • Latex-enhanced turbidimetric immunoassay kit for quantitatively detecting adiponectin ADPN and preparation and application methods thereof
  • Latex-enhanced turbidimetric immunoassay kit for quantitatively detecting adiponectin ADPN and preparation and application methods thereof
  • Latex-enhanced turbidimetric immunoassay kit for quantitatively detecting adiponectin ADPN and preparation and application methods thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] A latex-enhanced immune turbidimetric kit for quantitatively detecting adiponectin ADPN in this embodiment includes adiponectin R1 reagent, adiponectin R2 reagent and adiponectin calibrator.

[0057] The components and corresponding contents of the adiponectin R1 reagent include: buffer A (carbonate buffer, pH 6.0) 20 mM, protective agent A (ovalbumin) 1 mg / mL, reaction enhancer (polyethylene glycol) Alcohol 4000) 1%, preservative (thimerosal) 0.03%, and the solvent is purified water.

[0058] The components and corresponding contents of the adiponectin R2 reagent include: buffer B (borate buffer, pH 7.0) 20 mM, protective agent B (bovine serum albumin) 1 mg / mL, preservative (thimerosal) 0.03% , sensitized polystyrene latex particles coated with anti-human adiponectin antibody 0.04%, and the solvent is purified water.

[0059] The components and corresponding contents of the adiponectin calibrator are: buffer C (borate buffer, pH 7) 20 mM, protective agent C (bovine se...

Embodiment 2

[0064] A latex-enhanced immune turbidimetric kit for quantitatively detecting adiponectin ADPN in this embodiment includes adiponectin R1 reagent, adiponectin R2 reagent and adiponectin calibrator.

[0065] The components and corresponding contents of the adiponectin R1 reagent include: buffer A (phosphate buffer or glycine buffer, pH 10) 200 mM, protective agent A (gelatin) 10 mg / mL, reaction enhancer (polyethylene glycol) Alcohol 8000 or polyethylene glycol 20000) 4%, preservative (Proclin300) 0.2%, and the solvent is purified water.

[0066] The components and corresponding contents of the adiponectin R2 reagent include: buffer B (carbonate buffer or phosphate buffer, pH 9;) 200 mM, protective agent B (ovalbumin) 10 mg / mL, preservative (Proclin300) 0.2%, sensitized polystyrene latex particles coated with anti-human adiponectin antibody 0.16%, and the solvent is purified water.

[0067] The components and corresponding contents included in the adiponectin calibrator are: bu...

Embodiment 3

[0072] A latex-enhanced immune turbidimetric kit for quantitatively detecting adiponectin ADPN in this embodiment includes adiponectin R1 reagent, adiponectin R2 reagent and adiponectin calibrator.

[0073] The components and corresponding contents included in the adiponectin R1 reagent are: buffer A (borate buffer or Tris-HCl buffer, pH 8.0) 50 mM, protective agent A (bovine serum albumin) 4 mg / mL, Reaction enhancer (polyethylene glycol 6000) 5%, preservative (sodium azide) 0.1%, and the solvent is purified water.

[0074] The components and corresponding contents included in the adiponectin R2 reagent are: buffer B (Tris-HCl buffer or glycine buffer, pH 8.0) 60 mM, protective agent B (bovine serum albumin) 5 mg / mL, preservative ( Sodium azide) 0.1%, sensitized polystyrene latex particles coated with anti-human adiponectin antibody 0.1%, and the solvent is purified water.

[0075] The components and corresponding contents included in the adiponectin calibrator are: buffer C ...

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Abstract

The invention provides a latex-enhanced turbidimetric immunoassay kit for quantitatively detecting adiponectin ADPN. The latex-enhanced turbidimetric immunoassay kit comprises an adiponectin R1 reagent and an adiponectin R2 reagent, wherein the adiponectin R1 reagent comprises a buffer solution A, a protective agent A, a reaction enhancer and a preservative; the adiponectin R2 reagent comprises abuffer solution B, a protective agent B, a preservative and sensitized polystyrene latex particles coated with an anti-human adiponectin antibody; the anti-human adiponectin antibody in the sensitizedpolystyrene latex particle coated with the anti-human adiponectin antibody is connected with the sensitized polystyrene latex particle through a streptavidin-biotin system. The invention also provides a preparation method and an application method of the latex-enhanced turbidimetric immunoassay kit for quantitatively detecting adiponectin ADPN. The kit has the advantages that the kit is convenient, rapid, high in sensitivity and specificity and capable of quantitatively and accurately detecting ADPN; and the kit is strong in instrument compatibility and low in detection cost, and makes up theclinical requirements on ADPN turbidimetric products.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a latex-enhanced immune turbidimetric kit for quantitatively detecting adiponectin ADPN and a preparation and application method thereof. Background technique [0002] Adiponectin (ADPN) is an endogenous biologically active polypeptide or protein secreted by adipocytes. Adiponectin is an insulin-sensitizing hormone (An Insulin-sensitizing Hormone), which can improve insulin resistance and arteriosclerosis in mice; studies in humans have found that adiponectin levels can predict type II diabetes and coronary heart disease, and has demonstrated antidiabetic, antiatherogenic, and inflammatory potential in clinical trials. [0003] Researchers have discovered a new compound that modulates adiponectin, thereby suggesting a new avenue for studying the mechanism of adiponectin function and insulin sensitivity. Insulin is a hormone secreted by beta cells of the pancreas. I...

Claims

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Application Information

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IPC IPC(8): G01N33/74G01N33/577G01N33/543
CPCG01N33/74G01N33/577G01N33/54346G01N2800/042G01N2800/324
Inventor 芮双印符修乐邵家祥
Owner ANHUI DAQIAN BIO ENG LIMITED
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