Latex-enhanced turbidimetric immunoassay detection method

A technology of immunoturbidimetry and detection method, which is applied in the field of latex-enhanced immunoturbidimetry detection, and achieves the effects of low equipment cost, small reagent volume, and high penetration rate

Inactive Publication Date: 2018-12-21
LUMIGENEX (SUZHOU) CO LTD
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Embodiment 1

[0055] Embodiment 1: detection of cystatin C in human serum, the specific steps are as follows:

[0056] (1) Prepare R1 reagent, wherein the concentration of polyethylene glycol 6000 is 20 g / L, and the concentration of Tris buffer is 0.05 mmol / L. (2) Dilute 0.04g of 100nm polystyrene latex microspheres with 5g of Tris buffer, add 0.025g of EDC for activation, wash with Tris buffer and add 0.1g of anti-human cystatin C antibody for coupling Link it to the surface of the latex microspheres to form a cystatin C antibody-latex microsphere complex, that is, the latex microspheres coupled with the antibody; then prepare the R2 reagent, wherein the latex microspheres coupled with the antibody The concentration is 1g / L, the mass concentration of NaN3 is 0.05%, and the concentration of Tris buffer is 0.05mmol / L.

[0057] (3) Dilute the cystatin C standard substance of known concentration to get the concentration of 1 / 6, 1 / 3, 1 / 2, 2 / 3, 5 / 6 and the original concentration (concentration ...

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Abstract

The invention relates to a latex-enhanced turbidimetric immunoassay detection method, comprising the steps of: respectively adding a sample to be detected and a plurality of standards with different concentrations into different micro-wells of a well plate of a microplate reader; sequentially adding an R1 reagent and an R2 reagent into each of the micro-wells; and putting the well plate into the microplate reader for detection, wherein the feeding volume ratio of the sample to be detected or the standards, the R1 reagent and the R2 reagent is 1: 10 to 20: 20 to 30, the R1 reagent comprises a surfactant, and the R2 reagent comprises a latex microsphere coupled with an antibody. According to the latex-enhanced turbidimetric immunoassay detection method, an absorbance values of immune-particle complexes is detected by the microplate reader, and up to 96 specimens can be detected at one time, so that the detection speed is fast; the operation is convenient; the amount of required reagentsis small; the equipment cost is low; the popularity rate is high; a high energy detection is easy to realize; and the method is suitable for general medical institutions and underdeveloped areas.

Description

technical field [0001] The invention relates to a latex-enhanced immune transmission turbidity detection method. Background technique [0002] Latex particle-enhanced turbidimetric immunoassay (LETIA) is a relatively stable and accurate homogeneous immune turbidimetric detection method for body fluid proteins that has emerged in recent years. The LETIA method uses monoclonal antibodies cross-linked on the surface of polymer latex microspheres, and when the antigen is combined with the cross-linked antibody microspheres, it can quickly gather together in a short time and change the absorbance of the reaction solution. Moreover, the change of the absorbance of the reaction solution has a linear relationship with the concentration of the tested antigen within a certain range, which can be used to reflect the concentration of the tested antigen. [0003] This method has many advantages over other commonly used detection methods for body fluid proteins: LETIA eliminates the cumb...

Claims

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Application Information

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IPC IPC(8): G01N33/546
CPCG01N33/54346
Inventor 何爱民
Owner LUMIGENEX (SUZHOU) CO LTD
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