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Cystatin C latex-particle-enhanced turbidimetry detection reagent kit and application thereof

A detection kit and latex-enhanced technology, which is applied in the field of immunological determination and analysis, can solve problems such as weak linear correlation and poor precision, and achieve the effects of shortening detection time, high sensitivity, and improving detection sensitivity

Active Publication Date: 2016-07-06
WUHAN LIFE ORIGIN BIOTECH LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current technology also has the problem of poor precision and weak linear correlation.

Method used

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  • Cystatin C latex-particle-enhanced turbidimetry detection reagent kit and application thereof
  • Cystatin C latex-particle-enhanced turbidimetry detection reagent kit and application thereof
  • Cystatin C latex-particle-enhanced turbidimetry detection reagent kit and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0075] The components and concentration ranges of the reagent R1 are:

[0076]

[0077] The components and concentration ranges of the reagent R2 are:

[0078]

[0079]

[0080] Reagent R1 is a colorless and transparent solution, reagent R2 is a uniform milky white solution, and the reference calibrator of Cystatin C reagent is also a colorless and transparent solution.

Embodiment 2

[0082] The components and concentration ranges of the reagent R1 are:

[0083]

[0084] The components and concentration ranges of the reagent R2 are:

[0085]

[0086] Reagent R1 is a colorless and transparent solution, R2 is a uniform milky white solution, and the reference calibrator of Cystatin C reagent is also a colorless and transparent solution.

[0087] Comparative example 1R1 reagent composition is identical with embodiment 1, and R2 reagent composition and embodiment 1 are except that latex diameter is different, and all the other compositions are identical, and R2 latex diameter is respectively: described large-diameter polystyrene latex particle average particle diameter is 400nm~ 600nm, the average particle diameter of the small-diameter polystyrene latex is 16nm~32nm, large-diameter polystyrene latex particles: small-diameter polystyrene latex particles=(7-10):(0-3).

[0088] Comparative example 2R1 reagent composition is identical with embodiment 2, and ...

Embodiment 1 and 2

[0107] table 3

[0108]

[0109]

[0110] 4. Comparison of the anti-interference performance of the two kits:

[0111] Add four kinds of interfering substances to form mixed serum in the Roche quality control product (marked value 8.2mg / l), and the measurement results are as follows: total bilirubin assay kit of the present invention and existing kit measure anti-interference performance comparison:

[0112] Table 4

[0113]

[0114] The above-mentioned technical solutions in the embodiments of the present application have at least the following technical effects or advantages:

[0115] (1), the kit of the present invention uses latex-enhanced immunoturbidimetry to measure cystatin C, which amplifies the detection signal, improves the detection sensitivity, and shortens the detection time.

[0116] (2), the present invention adopts the latex reagent of mixed particle size, wherein the latex particle ratio of small particle size is big, has improved linearity; Contai...

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Abstract

The invention provides a cystatin C latex-particle-enhanced turbidimetry detection reagent kit and application thereof.The reagent kit comprises a reagent R1 and a reagent R2.The reagent R1 is prepared from a buffer solution, inorganic salt, a surfactant, a preservative, a stabilizer and interference elimination protein.The reagent R2 is prepared from a buffer solution, inorganic salt, a surfactant, a preservative, a stabilizer and a polystyrene latex particle mixture, and the polystyrene latex particle mixture is interlinked with a cystatin C antibody.The reagent kit based on latex-particle-enhanced turbidimetric immunoassay (PETIA) can be generally applied to analysis of various full-automatic biochemical analyzers and is short in assay time, good in specificity, high in precision and good in accuracy when used.

Description

technical field [0001] The invention relates to the field of immunological determination and analysis, in particular to a cystatin C latex enhanced turbidity detection kit and its application. Background technique [0002] Since 1985, cystatin C (cystatin C) has been regarded as a good marker for detecting renal function, because it is not affected by many physiological and pathological factors, it is comparable to other markers of glomerular filtration rate (GFR). has many advantages over. cystatinC also plays a role in a series of physiological and pathological processes, and has important clinical significance. Cystatin C, a protein encoded by the CST3 gene, is a good marker for kidney function diagnosis and exists in all nuclear cells in the body. Cystatin C can freely filter the glomerular membrane and is an important indicator of glomerular rate filtration (GFR) function. [0003] The development of immunoassay technology dates back to the mid-20th century, when opt...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/539
CPCG01N33/539
Inventor 华权高来祥兵徐春雷
Owner WUHAN LIFE ORIGIN BIOTECH LTD
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