Myeloperoxidase (MPO) determination kit (by using latex enhanced turbidimetric immunoassay)

Abstract

The invention relates to a kit for determining myeloperoxidase (MPO) content in serum. The invention aims to solve the technical problem to overcome the defects in the background art and provides a kit for determining myeloperoxidase content by enhanced turbidimetric immunoassay, which has the advantages of no need of dilution of a sample, simplicity in operation, high accuracy, good repeatability and suitability and is applied to various full-automatic biochemical analyzers and various special protein instruments. The invention adopts a technical scheme that the kit for determining the myeloperoxidase content by enhanced turbidimetric immunoassay comprises the following components: a, a reagent R1 which comprises buffer solution, a preservative, an accelerating agent, inorganic salt, a surface active agenet and the balance of purified water; b, a reagent R2 which comprises buffer agent, antibody combined with anti-human myeloperoxidase, a preservative, wherein the diameter of latex microspheres is 60-150nm; c, a reference calibration material which comprises buffer solution, a stabilizing agent, a preservative, a recombinant human myeloperoxidase pure product in a certain amount determined by concentration requirement and the balance of purified water. By the reagent combination, the calibration curve of MPO content is established, thereby achieving rapid determination of the MPO content in the serum on the full-automatic biochemical analyzer or the special protein instrument.

Description

Technical field: [0001] The invention relates to the field of biotechnology, in particular to a kit for measuring the content of myeloperoxidase in human serum by using a latex-enhanced immunoturbidimetric method. Background technique [0002] The pathogenesis of acute coronary syndrome (ACS) involves endothelial dysfunction, inflammatory response, vulnerable plaque and plaque rupture, coronary artery spasm, platelet aggregation and thrombosis, myocardial ischemia and myocardial necrosis, etc. A series of factors, in which inflammation and oxidative stress run through the whole process from the formation of atherosclerosis to the development of unstable plaques with thin fibrous caps. At present, with the deepening research on the involvement of inflammation in the pathogenesis of ACS, pro-inflammatory mediators and The role of anti-inflammatory mediators in the occurrence and development of ACS has also received attention, and serum myeloperoxidase (MPO) is a representative...

AI Technical Summary

Problems solved by technology

[0003] The MPO detection methods currently used clinically are: radioimmunoassay (IRA), which can directly measure plasma, but this method has environmental pollution and certain radioactive radiation to the operator; The method is more sensitive and accurate, but the cost is relatively expensive, and it needs supporting equipment before it can be used; the enzyme immunoassay (ELISA) has the disadvantages of cumbersome operation, sample pretreatment, and long detection time.

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