Combined orientation agents optimized latex coupled antibody detection method of prealbumin (PA)

A prealbumin and antibody detection technology, which is applied in the field of medical detection and in vitro diagnosis, can solve the problems of low utilization rate of latex microspheres and uncontrollable coupling effect, so as to improve coupling effect, good linearity, and improve product quality. performance effect

Inactive Publication Date: 2017-03-22
王贤俊
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, the turbidimetric method has gradually become a routine method for the determination of serum PA in clinical laboratories because of its simplicity, rapidity, automation, and trace characteristics. The combination of turbidimetry and latex microspheres forms a derivative of the common immunoturbidimetric technique, which is a simpler, faster, more sensitive and stronger signal detection method than the immunoturbidimetric method, but this method also has its limitations Due to the uncontrollability of the coupling effect and the uncontrollability of the latex microspheres, the utilization rate of the latex microspheres is not high. Therefore, a method to improve the coupling effect between the antibody and the latex microspheres is found to improve the utilization rate of the latex microspheres and enhance the reaction effect It is an urgent technical problem to be solved in this field

Method used

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  • Combined orientation agents optimized latex coupled antibody detection method of prealbumin (PA)
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  • Combined orientation agents optimized latex coupled antibody detection method of prealbumin (PA)

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Embodiment 1. Preparation of prealbumin (PA) detection kit (1)

[0049] The preparation of PA latex particles is the same as the process mentioned in the technical scheme.

[0050] The specific components of the prealbumin (PA) detection kit are prepared as follows:

[0051]

[0052] 1) Determination of precision: 20 consecutive samples were taken for measurement, and the mean, standard deviation and coefficient of variation of the measured values ​​were calculated.

[0053] Table 1 Precision test results

[0054]

[0055] The coefficient of variation CV is usually used to measure the precision of a determination method, the smaller the CV value, the better the precision of the result of the determination method. For clinical chemistry tests, a method precision with a CV of less than 5% is generally accepted as acceptable. The CV value in Table 1 is less than 5%, indicating that the kit of the present invention has excellent precision.

[0056] 2) Measuremen...

Embodiment 2

[0070] Embodiment 2. Preparation of prealbumin (PA) detection kit (2)

[0071] The preparation of PA latex particles is the same as the process mentioned in the technical scheme.

[0072] The specific components of the prealbumin (PA) detection kit are prepared as follows:

[0073]

[0074] 1) Determination of precision: 20 consecutive samples were taken for measurement, and the mean, standard deviation and coefficient of variation of the measured values ​​were calculated.

[0075] Table 6 precision test results

[0076]

[0077]

[0078] The coefficient of variation CV is usually used to measure the precision of a determination method, the smaller the CV value, the better the precision of the result of the determination method. For clinical chemistry tests, a method precision with a CV of less than 5% is generally accepted as acceptable. The CV value in Table 6 is less than 5%, indicating that the kit of the present invention has excellent precision.

[0079] 2...

Embodiment 3

[0094] Embodiment 3. The performance index comparison of kit of the present invention and commercially available kit A:

[0095] Preparation of prealbumin (PA) detection kit:

[0096] The preparation of PA latex particles is the same as the process mentioned in the technical scheme.

[0097] The specific components of the prealbumin (PA) detection kit are prepared as follows:

[0098]

[0099] The commercially available kit A of contrast, its information is as follows:

[0100] Product Name: Prealbumin Assay Kit (Sichuan Mike Technology Co., Ltd.)

[0101] Method: Immunoturbidimetry

[0102] Dosage form: liquid single reagent (R: 2×35ml)

[0103] Kit composition:

[0104] R:

[0105] PA reagent

[0106] Goat anti-human PA serum

[0107] Cellulite

[0108] STDs:

[0109] Prealbumin calibration serum

[0110] Basic parameters:

[0111] Method: endpoint method; sample / reagent: 1 / 100; main wavelength: 340nm; subwavelength: 700nm; reaction temperature: 37°C; reaction...

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Abstract

The invention provides a combined orientation agents optimized latex coupled antibody detection method of prealbumin (PA). The method is based on a latex enhanced turbidimetric immunoassay method, and is characterized in that combined orientation agents are utilized: an orientation agent 1 and an orientation agent 2. Firstly, the orientation agent 1 is combined with a PA antibody to form a PA antibody compound; the orientation agent 2 is then combined with the PA antibody compound; and finally, the compound is respectively coupled with carboxyl latex microspheres of two different particle sizes to form PA latex particles. By the method, the formed reagent has high sensitivity and good precision and linearity, is simple to prepare and is worthy of further promotion and application.

Description

[0001] Technical field: [0002] The invention relates to the fields of medical detection and in vitro diagnosis, and relates to a method for detecting prealbumin (PA) by combining a directional agent and optimizing a latex-coupled antibody. [0003] Background technique: [0004] Prealbumin (PA), also known as transthyretin, has a molecular weight of 54,000 and is synthesized by liver cells. It is often displayed in front of albumin during electrophoresis separation, and its half-life is very short, only about 1.9 days . Therefore, the determination of its concentration in plasma is more sensitive for understanding protein malnutrition and hepatic insufficiency than albumin and transferrin. In addition to being a sensitive nutritional protein indicator, the blood concentration of PA decreases in acute inflammation, malignant tumor, liver cirrhosis or nephritis. [0005] At present, there is no clinically recognized reference method for the determination of serum PA. The meth...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/577G01N33/546
CPCG01N33/68G01N33/54313G01N33/577
Inventor 王贤俊郑蓓蕾
Owner 王贤俊
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