Insoluble carrier particle nephelometric immunoassay reagent

Abstract

Turbidimetric immunoassay of insoluble carrier particles that can inhibit the agglutination reaction of insoluble carrier particles such as latex by inhibiting the effect of plasma components that affect the measured value, stabilize the agglutination reaction, stabilize the absorbance of the reaction solution, and obtain accurate measurement results A reagent for measurement, a kit for insoluble carrier particle turbidimetric immunoassay, and a method for insoluble carrier particle turbidimetric immunoassay using the reagent or kit. In the presence or absence of a buffer containing a compound containing the following groups in the molecule, such as broad bean pyrimidine glucoside, tricoflavone, etc., the insoluble carrier particles are loaded with antibodies or antigens, and then, in the presence of the above buffer , the insoluble carrier particle suspension sensitive to the antibody or antigen is in contact with the test body, and an immunoagglutination reaction occurs, and the concentration produced is measured through the agglutination reaction of the insoluble carrier particles, and the antigen or antibody in the test body is quantified. (In the formula, R1, R2, and R3 can be the same or different, and represent a hydrogen atom, a hydroxyalkyl group, etc.)

Description

technical field

[0001] The present invention provides a reagent for turbidimetric immunoassay containing insoluble carrier particles such as latex, a method for turbidimetric immunoassay using insoluble carrier particles, and a kit for turbidimetric immunoassay containing insoluble carrier particles. More specifically, a reagent for turbidimetric immunoassay containing insoluble carrier particles that stabilizes absorbance and suppresses the action of plasma components related to the reaction that affects the measured value, and a turbidimetric immunoassay using insoluble carrier particles Assay method and kit for turbidimetric immunoassay containing insoluble carrier particles. technical background

[0002] In the field of clinical examinations, latex is widely used in immunoassays for measuring antigens or antibodies in a sample. For example, Japanese Patent Laid-Open No. 10-253629 describes that in a buffer solution of pH 4.0 to 6.0, such as a phosphate citrate buffer so...

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