Insoluble carrier particle nephelometric immunoassay reagent
A carrier particle and immunoassay technology, applied in biological testing, measuring devices, instruments, etc., can solve problems such as unstable sensitivity and surface charge changes
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[0012] In the present invention, the insoluble carrier particle reagent for turbidimetric immunoassay is the insoluble carrier particle reagent for turbidimetric immunoassay containing insoluble carrier particles, a buffer comprising a compound having a group represented by [Chemical Formula 4] or a salt thereof in the molecule. , there is no special restriction. In addition, if the insoluble carrier particle turbidimetric immunoassay method of the present invention uses an insoluble carrier particle and a buffer formed of a compound having a group represented by [Chemical Formula 4] or a salt thereof in the molecule, the insoluble carrier particle turbidimetric immunoassay method, There are no special restrictions. Here, the insoluble carrier particle turbidimetric immunoassay method refers to a method of performing immunoagglutination reaction with insoluble carrier particles, measuring the concentration after the reaction, and quantifying the antigen or antibody in the samp...
Embodiment 1
[0033] Example 1 [Preparation of Reagent 1 (Buffer)]
[0034]As a buffer, 3.26 g of fabamidine glucoside (manufactured by Dojin Chemical Co., Ltd.) was dissolved in distilled water, and 0.1 g of surfactant (Triton) X-100, 17.5 g of sodium chloride, and 0.01 g of sodium azide were added. , while measuring the pH at 20°C, add 1 mol / L hydrochloric acid or sodium hydroxide aqueous solution to adjust the pH to 8.0, and add distilled water to make the total volume 1,000ml. Also available are 3.58 g of tricoflavone (manufactured by Tongren Chemical Co.), TAPSO {2-hydroxy-3-[N-tris(hydroxymethyl)methylamino]propanesulfonic acid} (manufactured by Tongren Chemical Co.) 5.18 g, POPSO [piperazine -1,4-bis(2-hydroxy-3-propanesulfonic acid) dihydrate] (manufactured by Dojin Chemical Co., Ltd.) 7.97 g, TES{2-[N-tris(hydroxymethyl)methylamino]propanesulfonic acid} (Manufactured by Dojin Chemical Co., Ltd.) 4.59 g instead of faba bean pyrimidine glucoside was dissolved in distilled water as a...
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