Insoluble carrier particle nephelometric immunoassay reagent

A carrier particle and immunoassay technology, applied in biological testing, measuring devices, instruments, etc., can solve problems such as unstable sensitivity and surface charge changes

Inactive Publication Date: 2003-09-17
KYOWA MEDEX CO LTD +1
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Problems solved by technology

[0005] As mentioned above, latex is often used in the measurement system of immunoagglutination reaction such as latex turbidimetric immunoassay, and the latex suspension refers to the reaction of plasma components coexisting with the reaction solution by mixing a trace amount of heavy metal ions etc. into it. , to change the adsorption of the latex carrier of the antigen or antibody, or to dissociate the antigen or anti

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  • Insoluble carrier particle nephelometric immunoassay reagent

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Embodiment approach

[0012] In the present invention, the insoluble carrier particle reagent for turbidimetric immunoassay is the insoluble carrier particle reagent for turbidimetric immunoassay containing insoluble carrier particles, a buffer comprising a compound having a group represented by [Chemical Formula 4] or a salt thereof in the molecule. , there is no special restriction. In addition, if the insoluble carrier particle turbidimetric immunoassay method of the present invention uses an insoluble carrier particle and a buffer formed of a compound having a group represented by [Chemical Formula 4] or a salt thereof in the molecule, the insoluble carrier particle turbidimetric immunoassay method, There are no special restrictions. Here, the insoluble carrier particle turbidimetric immunoassay method refers to a method of performing immunoagglutination reaction with insoluble carrier particles, measuring the concentration after the reaction, and quantifying the antigen or antibody in the samp...

Embodiment 1

[0033] Example 1 [Preparation of Reagent 1 (Buffer)]

[0034]As a buffer, 3.26 g of fabamidine glucoside (manufactured by Dojin Chemical Co., Ltd.) was dissolved in distilled water, and 0.1 g of surfactant (Triton) X-100, 17.5 g of sodium chloride, and 0.01 g of sodium azide were added. , while measuring the pH at 20°C, add 1 mol / L hydrochloric acid or sodium hydroxide aqueous solution to adjust the pH to 8.0, and add distilled water to make the total volume 1,000ml. Also available are 3.58 g of tricoflavone (manufactured by Tongren Chemical Co.), TAPSO {2-hydroxy-3-[N-tris(hydroxymethyl)methylamino]propanesulfonic acid} (manufactured by Tongren Chemical Co.) 5.18 g, POPSO [piperazine -1,4-bis(2-hydroxy-3-propanesulfonic acid) dihydrate] (manufactured by Dojin Chemical Co., Ltd.) 7.97 g, TES{2-[N-tris(hydroxymethyl)methylamino]propanesulfonic acid} (Manufactured by Dojin Chemical Co., Ltd.) 4.59 g instead of faba bean pyrimidine glucoside was dissolved in distilled water as a...

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Abstract

Turbidimetric immunoassay of insoluble carrier particles that can inhibit the agglutination reaction of insoluble carrier particles such as latex by inhibiting the effect of plasma components that affect the measured value, stabilize the agglutination reaction, stabilize the absorbance of the reaction solution, and obtain accurate measurement results A reagent for measurement, a kit for insoluble carrier particle turbidimetric immunoassay, and a method for insoluble carrier particle turbidimetric immunoassay using the reagent or kit. In the presence or absence of a buffer containing a compound containing the following groups in the molecule, such as broad bean pyrimidine glucoside, tricoflavone, etc., the insoluble carrier particles are loaded with antibodies or antigens, and then, in the presence of the above buffer , the insoluble carrier particle suspension sensitive to the antibody or antigen is in contact with the test body, and an immunoagglutination reaction occurs, and the concentration produced is measured through the agglutination reaction of the insoluble carrier particles, and the antigen or antibody in the test body is quantified. (In the formula, R1, R2, and R3 can be the same or different, and represent a hydrogen atom, a hydroxyalkyl group, etc.)

Description

technical field [0001] The present invention provides a reagent for turbidimetric immunoassay containing insoluble carrier particles such as latex, a method for turbidimetric immunoassay using insoluble carrier particles, and a kit for turbidimetric immunoassay containing insoluble carrier particles. More specifically, a reagent for turbidimetric immunoassay containing insoluble carrier particles that stabilizes absorbance and suppresses the action of plasma components related to the reaction that affects the measured value, and a turbidimetric immunoassay using insoluble carrier particles Assay method and kit for turbidimetric immunoassay containing insoluble carrier particles. technical background [0002] In the field of clinical examinations, latex is widely used in immunoassays for measuring antigens or antibodies in a sample. For example, Japanese Patent Laid-Open No. 10-253629 describes that in a buffer solution of pH 4.0 to 6.0, such as a phosphate citrate buffer so...

Claims

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Application Information

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IPC IPC(8): G01N33/543
CPCG01N33/54313G01N33/54393G01N33/543
Inventor 重信香代子小栗一人
Owner KYOWA MEDEX CO LTD
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