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Kit for determining Lp-PLA2 based on latex particle-enhanced turbidimetric immunoassay and preparation method of kit

An immunoturbidimetric and latex-enhanced technology, which is used in measurement devices, instruments, disease diagnosis, etc., can solve the problems of poor specificity and low sensitivity, achieve high accuracy, meet clinical needs, and improve sensitivity and specificity.

Inactive Publication Date: 2018-05-08
海格德生物科技(深圳)有限公司
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  • Summary
  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to provide a kind of kit and its preparation method based on latex-enhanced immune turbidimetry assay Lp-PLA2, aiming to solve the poor specificity when adopting latex-enhanced immune turbidimetry to measure Lp-PLA2 in the prior art and the problem of low sensitivity

Method used

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  • Kit for determining Lp-PLA2 based on latex particle-enhanced turbidimetric immunoassay and preparation method of kit
  • Kit for determining Lp-PLA2 based on latex particle-enhanced turbidimetric immunoassay and preparation method of kit
  • Kit for determining Lp-PLA2 based on latex particle-enhanced turbidimetric immunoassay and preparation method of kit

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preparation example Construction

[0027] In addition, the present invention also provides a method for preparing a test kit based on latex-enhanced immune turbidimetry for measuring Lp-PLA2, which includes the following steps:

[0028] S100, measure the latex microspheres with a solid content of 10%, add to the buffer, centrifuge, remove the supernatant, resuspend the latex microsphere sediment with buffer, add carbodiimide (also known as EDC) and N -Hydroxysuccinimide (aka NHS), activated. The weight ratio of carbodiimide and N-hydroxysuccinimide added in the present invention is 1:5-1:50; centrifuge again, remove the supernatant, and resuspend the latex microsphere precipitate with buffer , to prepare activated latex microspheres. There are mainly three operations in step S100: centrifugation to remove supernatant, activation, and resuspension.

[0029] S200. Take at least two kinds of monoclonal antibodies and mix them to prepare an antibody containing multiple monoclonal antibodies. In this step, by scr...

specific Embodiment approach

[0036] Below in conjunction with specific embodiment, further illustrate the present invention. It should be understood that these examples are only used to illustrate the present invention and are not intended to limit the scope of the present invention. In addition, it should be understood that after reading the content of the present invention, those skilled in the art may make various changes or modifications to the present invention, and these equivalent forms also fall within the scope defined by the appended claims of the present application.

[0037] 1. Preparation of Lp-PLA2 reagent R1 (i.e. reaction solution):

[0038] Accurately weigh the chemical substances according to the ratio in List 1, place them in a beaker filled with 0.9L pure water, stir and mix well, adjust the pH value to 7.20±0.05 with 1mol / L dilute hydrochloric acid, and transfer the solution to Dilute to volume in a 1L volumetric flask, filter the solution with a 0.22-micron membrane filter, store th...

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Abstract

The invention provides a kit for determining Lp-PLA2 based on latex particle-enhanced turbidimetric immunoassay and a preparation method of the kit. The kit comprises reaction liquid and a latex antibody reagent, wherein the latex antibody reagent comprises at least two latex antibodies formed by coupling monoclonal antibodies with latex microspheres. The preparation method comprises the followingsteps: screening out Lp-PLA2 monoclonal antibodies with higher specificity, and mixing multiple monoclonal antibodies instead of polyclonal antibodies to carry out latex coupling to obtain the latexantibodies; then preparing the reaction liquid; finally, forming the kit by using the latex antibodies and the reaction liquid, wherein the kit is used for determining the content of the Lp-PLA2. By using the kit prepared by the preparation method provided by the invention, sensitivity and specificity of determining the Lp-PLA2 by the latex particle-enhanced turbidimetric immunoassay can be simultaneously improved; further, the kit has the advantages of wider linear range, higher accuracy, extremely-high economic value and broad market application prospect.

Description

technical field [0001] The invention relates to the field of preparation of biological detection reagents, in particular to a kit for measuring Lp-PLA2 based on latex-enhanced immune turbidimetry and a preparation method thereof. Background technique [0002] Cardiovascular and cerebrovascular disease is a general term for cardiovascular and cerebrovascular diseases. Cardiovascular and cerebrovascular diseases have the characteristics of "high incidence, high disability rate, high mortality rate, high recurrence rate, and many complications", that is, "four highs and one many". . At present, domestic and foreign guidelines recommend the use of traditional risk factor-based models to predict the short-term and long-term risks of atherosclerotic cardiovascular disease. However, there are still deficiencies in using only traditional risk factors. Therefore, biomarkers are considered to be an important supplement to traditional risk assessment. [0003] Plasma lipoprotein-ass...

Claims

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Application Information

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IPC IPC(8): G01N33/573G01N33/531
CPCG01N33/531G01N33/573G01N2333/916G01N2800/226G01N2800/323
Inventor 卢岳军陈雪仇家舟王朝阳陈晶晶
Owner 海格德生物科技(深圳)有限公司
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