Lipoprotein (a) detection kit

Abstract

The invention relates to a lipoprotein (a) detection kit which comprises a reagent R1, a reagent R2 and a reference substance, wherein the reagent R1 is an HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) buffer system which comprises 0.5-10 g / L HEPES, 2-20 g / L sodium chloride, 0.05-1.0 ml / L Tween-20, 0.1-2 g / L bovine serum albumin, 5-25 g / L polyethyleneglycol-6000, 1-5 g / L EDTA (ethylene diamine tetraacetic acid) and 0.1-2 g / L Proclin 300; the reagent R2 comprises a polystyrene latex particle mixture which is prepared by a chemical crosslinking method, coated with an anti-human-apolipoprotein (a) polyclonal antibody and provided with carboxylic groups on the surface, a 0.5-10 g / L HEPES buffer solution and aspartame, wherein the weight-to-volume ratio of the aspartame to the reagent R2 is (0.01-0.5):100 (g / L); and the reference substance is a human serum or serum-matrix-like liquid with human recombinant apolipoprotein (a). The kit provided by the invention has the advantages of high detection sensitivity, high accuracy, favorable precision, favorable linearity within detection range, high stability, low production cost and low blank absorbance, and has anti-interference performance.

Description

technical field [0001] The invention relates to a medical detection kit, in particular to a kit for measuring lipoprotein (a) in human serum by using latex-enhanced immune turbidimetry. Background technique [0002] Lipoprotein (a) (Lipoprotein (a), LP (a)) is a lipoprotein similar to low-density lipoprotein, and its core part contains triglycerides, phospholipids, and cholesterol similar to low-density lipoprotein components , cholesteryl ester and other lipids and apolipoprotein B100 (ApoB100), also contains apolipoprotein (a) (Apolipoprotein (a), Apo (a)) which is not found in low-density lipoprotein. Therefore, the content of lipoprotein (a) in serum is usually reflected by detecting the concentration of apolipoprotein (a) in medicine. The cDNA sequence of apolipoprotein (a) was reported in 1993, and the primary structure of the protein was deduced, and its relative molecular mass was found to be (250-800kDa), containing 4529 amino acids. Apolipoprotein (a) is linked t...

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Problems solved by technology

[0003] At present, known methods for detecting the concentration of lipoprotein (a) include enzyme-linked immunoassay, radioimmunoassay, fluorescent immunoassay and other detection methods. The operation of the above-mentioned determination methods is relatively complicated, the detection takes a long time, and the shortcomings of excessive waste of resources , not suitable for routine testing

A lipoprotein (a) detection kit is disclosed on the website of the State Intellectual Property Office of China. It adopts latex-enhanced immunoturbidimetry, which has the advantages of good stability and convenient detection. However, the rheumatoid factor contained in human serum is harmful to the The detection value of the kit is prone to interference, which makes the measured value of individual serum with high content of rheumatoid factor appear false positive

In addition, the existing latex-enhanced immunoturbidimetric method uses physical adsorption to label polyclonal antibodies on polystyrene latex particles without carboxyl groups. The polystyrene latex particles without carboxyl groups have poor stability and physical The effect of adsorption is also not good, it is easy to cause the antibody to fall off from the surface of latex particles

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