The invention discloses a chemiluminescence enzyme-linked immunoassay detection kit for Lp-PLA2 (lipoprotein-associated phospholipaseA2). The kit is characterized by comprising a reagent R1 and a reagent R2, wherein the reagent R1 is a 0.25M ammonium chloride buffer solution containing 5 g/L polyethylene glycol 8000, 10 g/L sodium chloride, 4 g/L EDTA (ethylene diamine tetraacetic acid), 8 g/L saccharose, 2 g/L bovine serum albumin, 5 g/L Tween 20 and 1 g/L sodium azide; the reagent R2 is a 0.1M ammonium chloride buffer solution containing a polystyrene latex particle mixture coated with anti-human apolipoprotein polyclonal antibodies and prepared with a chemical cross-linking method, a 3 g/L HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) buffer solution, aspartame and 2 g/L sodium azide, wherein the weight-to-volume ratio of aspartame to the reagent R2 is 0.2: 100 g/L, and the pH value is 9.0. The detection kit has high detection sensitivity, high accuracy, good precision, good linearity within the detection range, good stability, low production cost and low blank absorbency, and also has an anti-interference property.