31results about How to "Avoiding the problem of false positives" patented technology

The invention discloses an intelligent early warning system for the closing of a train and an early warning method of the intelligent early warning system. The intelligent early warning system comprises an early warning server. The early warning method comprises the following steps: receiving train position information transmitted by vehicle-mounted equipment through a public wireless network by the early warning server; transmitting early warning information of the closing of the train to a path opening terminal through the public wireless network by the early warning server, and besides receiving path opening position information collected by the path opening terminal; also transmitting the early warning information of the closing of the train to mobile equipment of working personnel through the public wireless network by the early warning server, and besides receiving position information collected by the working personnel or an operation point. The early warning server is arranged in a machine room and connected with accession equipment of the public wireless network through optical fibers and twisted-pair lines. The intelligent early warning system and the early warning method, which are disclosed by the invention, have the benefits of being suitable for a line zone with automatic blocking and an in-station accouterment monitoring system and being also suitable for full-automatic intelligent early warning system for the closing of the train in a non-automatic blocking zone.

The invention relates to a lipoprotein (a) detection kit which comprises a reagent R1, a reagent R2 and a reference substance, wherein the reagent R1 is an HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) buffer system which comprises 0.5-10 g / L HEPES, 2-20 g / L sodium chloride, 0.05-1.0 ml / L Tween-20, 0.1-2 g / L bovine serum albumin, 5-25 g / L polyethyleneglycol-6000, 1-5 g / L EDTA (ethylene diamine tetraacetic acid) and 0.1-2 g / L Proclin 300; the reagent R2 comprises a polystyrene latex particle mixture which is prepared by a chemical crosslinking method, coated with an anti-human-apolipoprotein (a) polyclonal antibody and provided with carboxylic groups on the surface, a 0.5-10 g / L HEPES buffer solution and aspartame, wherein the weight-to-volume ratio of the aspartame to the reagent R2 is (0.01-0.5):100 (g / L); and the reference substance is a human serum or serum-matrix-like liquid with human recombinant apolipoprotein (a). The kit provided by the invention has the advantages of high detection sensitivity, high accuracy, favorable precision, favorable linearity within detection range, high stability, low production cost and low blank absorbance, and has anti-interference performance.

The invention relates to a kit for auxiliary diagnosis of cervical carcinoma and high-grade cervical intraepithelial lesion, and discloses a kit for detecting high-risk HPV. The kit for detecting the high-risk HPV comprises a high-risk HPV nucleic acid fluorescent PCR detection mixture and Taq enzyme. The invention also discloses a method for preparing the kit for detecting the high-risk HPV and a method for using the same. The kit can be used for detecting the high-risk HPV and overcomes the defects of high false positive rate, low specificity, high cost and the like of typing detection of the high-risk HPV in the prior art.

The embodiment of the invention provides a loss prevention method, a loss prevention device and a loss prevention system and relates to the field of wireless signal transmission. The loss prevention method provided by the embodiment of the invention comprises the following steps: acquiring a first signal strength value of a first wireless signal transmitted by a wireless signal receiving and transmitting device by using the loss prevention device; measuring according to the first signal strength value to obtain the distance between the loss prevention device and the wireless signal receiving and transmitting device; judging whether the measured distance is larger than a preset detection distance, and sending an alarming signal if the measured distance is larger than the preset detection distance. By the loss prevention method, the loss prevention device and the loss prevention system provided by the embodiment of the invention, reliable guarantee is provided for preventing loss of a monitored object, and the problem that the existing monitored object is easy to lose can be solved.

The invention discloses a calcification point identification method. The method comprises the steps of obtaining a to-be-identified image; processing each pixel point of the image according to a preset first algorithm to obtain a suspected calcification candidate block of the image; and performing calcification point recognition on the suspected calcification candidate block of the image accordingto a preset second algorithm, calculating a calcification probability value of the suspected calcification candidate block, and determining whether the suspected calcification candidate block is calcified or not according to the calcification probability value. The invention also discloses a calcification point identification device and a computer readable storage medium. According to the method,a large amount of manpower can be reduced and a certain recognition rate can be ensured in the calcification point recognition process.

The invention discloses an unmanned supermarket shelf monitoring system and monitoring methods thereof. The method comprises the steps that after a camera device is powered on, the camera device carries out a preset photographing program, photographs a picture and uploads the photographed picture to a background server which is in communication connection with the camera device; the background server stores the picture photographed by the camera device; the background server carries out picture identification on the stored newest picture, after it is identified that the number of goods on a shelf is smaller than the preset number, the background server sends replenishment prompt information to a management terminal which is in communication connection with the background server; after thecamera device checks that the picture is uploaded successfully, the camera device is powered off automatically; and an RTC alarm clock built in the camera device wakes up the camera device again everyother a preset standby time interval, and the unmanned supermarket shelf monitoring system repeats the steps of the method according to the above-mentioned sequence. According to the system and the method, shelf goods number monitoring with ultra-low power consumption is realized and a replenishment prompt function is realized. The system and the method have the advantages of low power consumption, accurate identification and convenient installation.

The invention discloses a carp spring viremia virus detection kit based on pyrosequencing. The carp spring viremia virus detection kit based on the pyrosequencing depends on a carp spring viremia virus fingerprint sequence, and the carp spring viremia virus fingerprint sequence is nucleotides at the 511th-526th positions of SEQ ID No.1 shown in a sequence table. The kit comprises a sequencing primer of the carp spring viremia virus fingerprint sequence and a primer pair for amplifying the carp spring viremia virus fingerprint sequence. The sequencing primer is single-stranded DNA represented by SEQ ID No.4 shown in the sequence table. The primer pair for amplifying the carp spring viremia virus fingerprint sequence consists of single-stranded DNA represented by SEQ ID No.2 shown in the sequence table and single-stranded DNA represented by SEQ ID No.3 shown in the sequence table.

The invention discloses a shelf monitoring device for an unmanned supermarket. The shelf monitoring device comprises a camera, a processor, a communication module I coupled to the processor, a dial switch K2, a P-channel MOS tube Q2, a DC-DC synchronous buck converter, an NPN triode Q1, and an RTC alarm clock chip. The processor is coupled to an external serial port of the camera; and after the camera shoots an image, the image uploaded to a background server connected with the camera. The dial switch K2 has an input terminal and an output terminal; the input terminal is coupled to a direct current VIN and the output terminal is connected in series with an inductor R19 and a capacitor C6 successively and then is grounded. A grid G of the P-channel MOS tube Q2 is coupled between the inductor R19 and the capacitor C6 and a source S of the P-channel MOS tube Q2 is c coupled to the direct current VIN. A collector C of the NPN triode Q1 is coupled to the grid G of the P-channel MOS tube Q2and a base electrode B is connected in series with a resistor R1 and then is coupled to a power control off terminal PWR_HOLD of the processor. According to the invention, the ultra-low-power-consumption shelf goods number monitoring is realized. The shelf monitoring device having the cargo supplementing prompting function has advantages of low power consumption, accurate identification and greatconvenience in installation.

The invention discloses an infectious hematopoietic necrosis virus detection kit based on pyrosequencing. The infectious hematopoietic necrosis virus detection kit based on the pyrosequencing depends on an infectious hematopoietic necrosis virus fingerprint sequence, and the infectious hematopoietic necrosis virus fingerprint sequence is nucleotides at the 553th-565th positions of SEQ ID No.9 shown in a sequence table. The kit comprises a sequencing primer of the infectious hematopoietic necrosis virus fingerprint sequence and a primer pair for amplifying the infectious hematopoietic necrosis virus fingerprint sequence. The sequencing primer is single-stranded DNA represented by SEQ ID No.12 shown in the sequence table named as IHNV-S. The primer pair for amplifying the infectious hematopoietic necrosis virus fingerprint sequence consists of single-stranded DNA represented by SEQ ID No.10 shown in the sequence table named as IHNV-P and single-stranded DNA represented by SEQ ID No.11 shown in the sequence table.

The invention discloses a deer species source identification primer pair for cornua cervi pantotrichum products. The identification primer pair comprises a deer identification primer pair DcF-DcR anda cornua cervi pantotrichum identification primer pair Rdfp-Rdrp. The invention also provides an identification method of the deer species source identification primer pair for the cornua cervi pantotrichum products. The method comprises the following steps: S1, preparing a preservation solution of a sample; S2, extracting DNA; S3, identifying the tissue sample by a deer identification program; and S4, determining the sequence after identification by the identification program of cornua cervi pantotrichum to judge the specific species of the cornua cervi pantotrichum product. The identification primer pair has the beneficial effects that integrated and accurate identification of deer and cornua cervi pantotrichum products is achieved by the deer identification primer pair and the cornua cervi pantotrichum identification primer pair; by big data analysis of deer genome sequence, identification accuracy of the cornua cervi pantotrichum product is improved; by first deer identification and then the cornua cervi pantotrichum identification program, accurate identification is achieved; and multiple groups of controls are set, the purified and identified products are recovered by gel extraction, and sequencing and analyzing are performed, so that accurate identification is achieved.

The invention discloses an intelligent early warning system for the closing of a train and an early warning method of the intelligent early warning system. The intelligent early warning system comprises an early warning server. The early warning method comprises the following steps: receiving train position information transmitted by vehicle-mounted equipment through a public wireless network by the early warning server; transmitting early warning information of the closing of the train to a path opening terminal through the public wireless network by the early warning server, and besides receiving path opening position information collected by the path opening terminal; also transmitting the early warning information of the closing of the train to mobile equipment of working personnel through the public wireless network by the early warning server, and besides receiving position information collected by the working personnel or an operation point. The early warning server is arranged in a machine room and connected with accession equipment of the public wireless network through optical fibers and twisted-pair lines. The intelligent early warning system and the early warning method, which are disclosed by the invention, have the benefits of being suitable for a line zone with automatic blocking and an in-station accouterment monitoring system and being also suitable for full-automatic intelligent early warning system for the closing of the train in a non-automatic blocking zone.

The invention discloses a method for detecting microcystin-LR in water through high performance liquid fluorescence chromatography, in particular relates to the method for detecting the microcystin-LRin the water through the high performance liquid fluorescence chromatography and belongs to the field of a water environment monitoring technology or an agricultural monitoring technology. Accordingto the method, a sample is filtered, enriched, eluted and concentrated to obtain a constant volume based on the characteristics that fluorescent light can be stimulated by the microcystin-LR under theultraviolet irradiation; rapid, accurate and efficient detection of the microcystin-LR in a water body is realized by adopting the high performance liquid fluorescence chromatography; the method hasguiding significance on monitoring, management and protection of a water body pollution condition.

The invention discloses a chemiluminescence enzyme-linked immunoassay detection kit for Lp-PLA2 (lipoprotein-associated phospholipaseA2). The kit is characterized by comprising a reagent R1 and a reagent R2, wherein the reagent R1 is a 0.25M ammonium chloride buffer solution containing 5 g / L polyethylene glycol 8000, 10 g / L sodium chloride, 4 g / L EDTA (ethylene diamine tetraacetic acid), 8 g / L saccharose, 2 g / L bovine serum albumin, 5 g / L Tween 20 and 1 g / L sodium azide; the reagent R2 is a 0.1M ammonium chloride buffer solution containing a polystyrene latex particle mixture coated with anti-human apolipoprotein polyclonal antibodies and prepared with a chemical cross-linking method, a 3 g / L HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) buffer solution, aspartame and 2 g / L sodium azide, wherein the weight-to-volume ratio of aspartame to the reagent R2 is 0.2: 100 g / L, and the pH value is 9.0. The detection kit has high detection sensitivity, high accuracy, good precision, good linearity within the detection range, good stability, low production cost and low blank absorbency, and also has an anti-interference property.

The invention relates to the technical field of molecular detection, in particular to a molecular lateral chromatography detection method based on a CRISPR system and a detection test strip, according to the method, lateral chromatography visual detection is realized by arranging Cas protein and a fixed labeled probe on lateral chromatography test paper or accessories, and multi-index joint detection can also be realized. The labeled probe contains a nucleic acid probe and a marker which are connected with each other, the Cas protein contains a guide sequence capable of targeting to-be-detected molecules, and when the to-be-detected molecules are contained in the detection sample, the Cas protein is activated to cut the nucleic acid probe in the labeled probe, so that the marker originally fixed on the lateral chromatography test paper or accessory is released. According to the invention, the labeled probe is fixed, the labeled substance is released after being cut, and detection is carried out through a sandwich method or capture method test strip, so that the detection specificity is improved, and the false positive problem in the prior art is avoided.

The invention discloses a relocation and loopback detection method based on depth map coding. The relocation and loopback detection method utilizes a depth map shot by an RGB-D camera to combine the estimated camera pose to assist in judging loopback detection and repositioning, that is to utilize a random fern encoding method to encode the depth map, compares the encoding similarity of the current frame depth map with the encoding similarity of the historical key frame depth map while comparing the spatial position similarity of the camera when the two frames of images are shot by utilizing the camera poses estimated by the two frames of images, and judges whether the camera returns to the previous area or not according to the encoding similarity and the spatial position similarity of thecurrent frame and the historical frame. On the basis of an original method based on a color image, by combining the depth information brought by a novel sensor, the relocation and loopback detectionmethod provides more stable repositioning and loopback detection effects, and encodes the image, so that the information of the whole depth map is prevented from being considered, and the calculationamount is greatly reduced. Meanwhile, the camera pose is also considered, so that the problem of false positive judgment result is avoided.

The embodiment of the invention discloses a liquid level measurer for a mortar box or a mud box, which comprises a mounting support, a laser sensor, a signal acquisition unit and an output unit, wherein the mounting support is fixedly connected to the upper part of a box body of the mortar box or the mud box; the laser sensor is fixedly connected to the mounting support, and a laser of the laser sensor is positioned above a cavity of the mortar box or the mud box and is arranged downwards; the signal acquisition unit is connected with the laser sensor and is used for acquiring distance data signals obtained by the laser sensor; and the output unit is connected with the signal acquisition unit and is used for outputting the signals acquired by the signal acquisition unit. Because the adopted laser sensor is not necessary to contact mortar or mud, the liquid level measurer solves the problem that in the prior art, the liquid level measurer generates misreports because an electrode bar is coated with the mortar or the mud to form a conducting state.

The invention provides a sample solution fixed-value sampling apparatus. The sample solution fixed-value sampling apparatus comprises a sample inlet pipeline, a sample inlet valve arranged on the sample inlet pipeline, a conveying unit and at least one sensor, wherein a plurality of photoelectric couplers are arranged in the sensor, when in sampling, after a sample solution passes through the sample inlet valve to reach the sensor, the sensor transmits a signal to a control unit, the control unit controls the sample inlet valve to be closed, a fixed-value sample solution between the sample inlet valve and the sensor is obtained, a photoelectric coupler calibration unit is provided before the sampling, the photoelectric coupler calibration unit can remove a photoelectric coupler interfered by residual liquid on the inner wall of the sample inlet pipeline so as to prevent the influence of misinformation on the sample solution fixed-value sampling can be prevented. The invention also provides a sample solution fixed-value sampling method. The sample solution fixed-value sampling apparatus and method have the advantages of high sampling accuracy and small interference.

The invention provides an earphone socket assembly and an electronic device. The earphone socket assembly comprises a socket main body which is provided with a socket hole. The detecting part is arranged at the bottom of the socket, and the headphone head is inserted into the socket to cause deformation of the detecting part; A detection circuit is connected to a detection section for determiningan insertion / withdrawal state of the headphone head based on an electrical signal generated by deformation of the detection section. The earphone socket assembly of the present invention the detectionpart is arranged at the bottom of the socket, The detection circuit can determine the insertion or withdrawal state of the headphone head based on the electric signal of the deformation, and the design of the earphone socket assembly can reduce the false alarm phenomenon caused by the conductive foreign body, thereby improving the judgment accuracy of the headphone head insertion and withdrawal.

The invention relates to the technical field of cables, particularly relates to an intelligent loss measurement cable. The cable is a sequentially connected segmented cable. Each section of the cablecomprises an outer sheath layer, a steel tape armoring layer, an inner sheath layer and conductors which are sequentially arranged from outside to inside; a filler is arranged between the conductors and the inner sheath layer; the filler is made of an acrylic polymer; the steel tape armoring layer is defined by a plurality of rubber cushion blocks which are annularly arranged; the number of the conductors is three, and each conductor is externally provided with an inner shielding layer, an insulating layer, an outer shielding layer, a copper tape shielding layer and an optical fiber unit protection layer which are sequentially arranged from inside to outside; and an optical fiber is arranged in the optical fiber unit protection layer.

A nucleic acid intramolecular amplification probe and detection method, the nucleic acid intramolecular amplification probe of the present invention contains at least five regions (5')a-b-a-c-a*(3'), Contains two identical a-regions, one at the 5' end and one in the middle; b-region is connected to two a-regions, one of a-region is connected at the 5' end to the 5' end of b-region, and the other a-region is connected to c-region The 5' end of the c region is connected to the 5' end of the a* region, and the a* region is the complementary nucleotide sequence of the a region and is at the 3' end of the probe; under the action of the polymerase, the probe takes itself as Templates and primers for intramolecular amplification of nucleic acids. In the invention, the template and the primer are designed in one molecule, and the same template is used to realize intramolecular amplification of the nucleic acid, effectively avoiding the problem of false positives. The present invention also includes a method for detecting target nucleic acid using probes and probe precursors. The method can detect single base difference, has strong anti-interference ability, and has broad market prospect.

The invention relates to a deamidated gliadin polypeptide recombinant antigen, a recombinant antigen expression gene, a recombinant expression vector and a preparation method and application thereof. The deamidated gliadin polypeptide recombinant antigen includes DGP‑1 peptide, interleukin 15 protein and DGP‑2 peptide, the amino acid sequence of the DGP‑1 peptide is shown in SEQ ID NO.1, and the DGP‑2 The amino acid sequence of the peptide is shown in SEQ ID NO.2. The deamidated gliadin polypeptide recombinant antigen provided by the present invention has good sensitivity, high specificity and low preparation cost when used in the detection of celiac disease, can detect IgG-DGP antibody in serum, and can especially be used for IgA-deficient patients and infants. Detection of patients with potential celiac disease in a young child population.

The invention is suitable for the technical field of railway vehicle detection, and provides a railway vehicle compressed air humidity detection and display device which comprises an air pipeline and a train control and management system. The air pipeline is provided with a compressed air humidity detection module, the compressed air humidity detection module is electrically connected with the train control and management system, the compressed air humidity detection module further comprises a box body with an opening in the front face and an air circuit board, and the air circuit board is matched with the opening in the front face of the box body. According to the railway vehicle compressed air humidity detection and display device, the humidity of compressed air can be detected and displayed according to pressure setting, so that the problem of false alarm is avoided, and whether the humidity of the compressed air is qualified or not can be indicated through a red lamp or a green lamp of an indicator lamp according to the air humidity; it is convenient for maintenance manual to observe and check, a pressure switch and a humidity sensor can be calibrated at any time through external equipment, so that manual operation is facilitated.

The invention relates to the technical field of garbage cans, in particular to an intelligent compression garbage can, which includes an outer cylinder, an inner cylinder, a motor and a rotating shaft. The side wall of the outer cylinder is provided with a garbage input port and a garbage cleaning port, and the bottom of the inner cylinder is fixed with a The rotating shaft is connected to the outer cylinder in rotation, and the rotating shaft is connected to the output shaft of the motor. The side wall of the inner cylinder is provided with a water hole. , There is a fixed plate on the top of the outer cylinder, a threaded hole is provided on the fixed plate, a threaded rod is fixedly connected to the extruded plate, the threaded rod is connected to the fixed plate by threads, and an elastic water bag is arranged between the fixed plate and the extruded plate , a liquid storage tank is arranged above the fixing plate, and the liquid storage tank is used to hold disinfectant, a first one-way valve is arranged on the elastic water bag, and a second one-way valve is arranged on the extrusion plate. The invention provides an intelligent compressed garbage can, which can notify the user after the garbage can is full, and there is no false alarm problem caused by irregular garbage.

The invention discloses a viral hemorrhagic septicemia virus detection kit based on pyrosequencing. The viral hemorrhagic septicemia virus detection kit based on the pyrosequencing depends on a viral hemorrhagic septicemia virus fingerprint sequence, and the viral hemorrhagic septicemia virus fingerprint sequence is nucleotides at the 451th-467th positions of SEQ ID No.5 shown in a sequence table. The kit comprises a sequencing primer of the viral hemorrhagic septicemia virus fingerprint sequence and a primer pair for amplifying the viral hemorrhagic septicemia virus fingerprint sequence. The sequencing primer is single-stranded DNA represented by SEQ ID No.8 shown in the sequence table named as VHSV-S. The primer pair for amplifying the viral hemorrhagic septicemia virus fingerprint sequence consists of single-stranded DNA represented by SEQ ID No.6 shown in the sequence table named as VHSV-P and single-stranded DNA represented by SEQ ID No.7 shown in the sequence table.

The invention provides a method and device for generating a dynamic threshold value for equipment early warning based on a statistical model. The method comprises the steps that historical data of recent (n + 1) days of operation of multiple devices are acquired, and n is a natural number larger than 1; processing the historical data of the recent (n + 1) days, and generating device objects for the plurality of devices; processing the device object based on a statistical model, and generating a fitting adjustment value for the result objects of the plurality of devices; and generating respective dynamic thresholds for the plurality of devices for device early warning by using the result objects for the plurality of devices. According to the method, the dynamic threshold prediction precision is improved; the mathematical models trained by the multiple devices are packaged in the result object, so that network transmission is facilitated, and the storage space occupied by the mathematical models is reduced.

The invention discloses a train wheel ultrasonic flaw detection method and system. The method comprises the following steps: acquiring a first image formed by an ultrasonic B-scan image of a train wheel; eliminating step echo lines and spoke plate hole echo lines in the first image to generate a second image and a step echo sound path corresponding to the step echo lines; performing binarization processing on the second image, and extracting a first connected domain set meeting condition flaw detection; and carrying out spatial distribution divergence discrimination on the first connected domain set and generating a second connected domain set used for representing the defect part. According to the invention, after the ultrasonic B-scan image is converted into a binary black-and-white image, interference echoes generated by foreign matters attached to the outer surface of a rim spoke transition region of the train wheel are identified and eliminated by calculating the spatial distribution dispersion of a black connected domain, so that the problem of false alarm of a train wheel ultrasonic flaw detection system due to foreign matters attached to the outer surface of the transitionarea is avoided, and the problem that a traditional train wheel ultrasonic flaw detection method is low in detection result reliability is solved.

The invention relates to a lipoprotein (a) detection kit which comprises a reagent R1, a reagent R2 and a reference substance, wherein the reagent R1 is an HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) buffer system which comprises 0.5-10 g / L HEPES, 2-20 g / L sodium chloride, 0.05-1.0 ml / L Tween-20, 0.1-2 g / L bovine serum albumin, 5-25 g / L polyethyleneglycol-6000, 1-5 g / L EDTA (ethylene diamine tetraacetic acid) and 0.1-2 g / L Proclin 300; the reagent R2 comprises a polystyrene latex particle mixture which is prepared by a chemical crosslinking method, coated with an anti-human-apolipoprotein (a) polyclonal antibody and provided with carboxylic groups on the surface, a 0.5-10 g / L HEPES buffer solution and aspartame, wherein the weight-to-volume ratio of the aspartame to the reagent R2 is (0.01-0.5):100 (g / L); and the reference substance is a human serum or serum-matrix-like liquid with human recombinant apolipoprotein (a). The kit provided by the invention has the advantages of high detection sensitivity, high accuracy, favorable precision, favorable linearity within detection range, high stability, low production cost and low blank absorbance, and has anti-interference performance.

The invention discloses an unmanned supermarket shelf monitoring system and monitoring methods thereof. The method comprises the steps that after a camera device is powered on, the camera device carries out a preset photographing program, photographs a picture and uploads the photographed picture to a background server which is in communication connection with the camera device; the background server stores the picture photographed by the camera device; the background server carries out picture identification on the stored newest picture, after it is identified that the number of goods on a shelf is smaller than the preset number, the background server sends replenishment prompt information to a management terminal which is in communication connection with the background server; after thecamera device checks that the picture is uploaded successfully, the camera device is powered off automatically; and an RTC alarm clock built in the camera device wakes up the camera device again everyother a preset standby time interval, and the unmanned supermarket shelf monitoring system repeats the steps of the method according to the above-mentioned sequence. According to the system and the method, shelf goods number monitoring with ultra-low power consumption is realized and a replenishment prompt function is realized. The system and the method have the advantages of low power consumption, accurate identification and convenient installation.

The invention discloses a kit for detecting the drug-resistant site of IFVA, a preparation thereof and an application thereof. The kit for detecting the drug-resistant site of the IFVA comprises a mixed IFVA-H274Y fluorescent PCR detection solution, an RT-PCR enzyme, a positive comparison product, and a negative comparison product, wherein the mixed IFVA-H274Y fluorescent PCR detection solution comprises primers, a probe, RT-PCR MIX and water. The kit for detecting the drug-resistant site of the IFVA is used for the drug-resistant analysis and discrimination of the IFVA to Tamiflu.

The invention relates to an auxiliary diagnostic kit for cervical cancer and high-grade intraepithelial lesions of the cervix, and discloses a kit for detecting high-risk human papillomaviruses, including high-risk HPV nucleic acid fluorescent PCR detection mixture and Taq enzyme. The invention also discloses the preparation method and application method of the kit for detecting high-risk human papillomavirus. The kit of the invention can be used to detect high-risk human papillomaviruses, and overcomes the problems of high false positive rate, low specificity, high cost and the like in prior art for detection of high-risk human papillomavirus (HPV) typing.