31results about How to "Avoiding the problem of false positives" patented technology

A nucleic acid intramolecular amplification probe and detection method, the nucleic acid intramolecular amplification probe of the present invention contains at least five regions (5')a-b-a-c-a*(3'), Contains two identical a-regions, one at the 5' end and one in the middle; b-region is connected to two a-regions, one of a-region is connected at the 5' end to the 5' end of b-region, and the other a-region is connected to c-region The 5' end of the c region is connected to the 5' end of the a* region, and the a* region is the complementary nucleotide sequence of the a region and is at the 3' end of the probe; under the action of the polymerase, the probe takes itself as Templates and primers for intramolecular amplification of nucleic acids. In the invention, the template and the primer are designed in one molecule, and the same template is used to realize intramolecular amplification of the nucleic acid, effectively avoiding the problem of false positives. The present invention also includes a method for detecting target nucleic acid using probes and probe precursors. The method can detect single base difference, has strong anti-interference ability, and has broad market prospect.

The invention relates to a lipoprotein (a) detection kit which comprises a reagent R1, a reagent R2 and a reference substance, wherein the reagent R1 is an HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) buffer system which comprises 0.5-10 g / L HEPES, 2-20 g / L sodium chloride, 0.05-1.0 ml / L Tween-20, 0.1-2 g / L bovine serum albumin, 5-25 g / L polyethyleneglycol-6000, 1-5 g / L EDTA (ethylene diamine tetraacetic acid) and 0.1-2 g / L Proclin 300; the reagent R2 comprises a polystyrene latex particle mixture which is prepared by a chemical crosslinking method, coated with an anti-human-apolipoprotein (a) polyclonal antibody and provided with carboxylic groups on the surface, a 0.5-10 g / L HEPES buffer solution and aspartame, wherein the weight-to-volume ratio of the aspartame to the reagent R2 is (0.01-0.5):100 (g / L); and the reference substance is a human serum or serum-matrix-like liquid with human recombinant apolipoprotein (a). The kit provided by the invention has the advantages of high detection sensitivity, high accuracy, favorable precision, favorable linearity within detection range, high stability, low production cost and low blank absorbance, and has anti-interference performance.

The invention discloses an unmanned supermarket shelf monitoring system and monitoring methods thereof. The method comprises the steps that after a camera device is powered on, the camera device carries out a preset photographing program, photographs a picture and uploads the photographed picture to a background server which is in communication connection with the camera device; the background server stores the picture photographed by the camera device; the background server carries out picture identification on the stored newest picture, after it is identified that the number of goods on a shelf is smaller than the preset number, the background server sends replenishment prompt information to a management terminal which is in communication connection with the background server; after thecamera device checks that the picture is uploaded successfully, the camera device is powered off automatically; and an RTC alarm clock built in the camera device wakes up the camera device again everyother a preset standby time interval, and the unmanned supermarket shelf monitoring system repeats the steps of the method according to the above-mentioned sequence. According to the system and the method, shelf goods number monitoring with ultra-low power consumption is realized and a replenishment prompt function is realized. The system and the method have the advantages of low power consumption, accurate identification and convenient installation.

The invention relates to an auxiliary diagnostic kit for cervical cancer and high-grade intraepithelial lesions of the cervix, and discloses a kit for detecting high-risk human papillomaviruses, including high-risk HPV nucleic acid fluorescent PCR detection mixture and Taq enzyme. The invention also discloses the preparation method and application method of the kit for detecting high-risk human papillomavirus. The kit of the invention can be used to detect high-risk human papillomaviruses, and overcomes the problems of high false positive rate, low specificity, high cost and the like in prior art for detection of high-risk human papillomavirus (HPV) typing.