Method for detecting microcystin-LR in water through high performance liquid fluorescence chromatography

Abstract

The invention discloses a method for detecting microcystin-LR in water through high performance liquid fluorescence chromatography, in particular relates to the method for detecting the microcystin-LRin the water through the high performance liquid fluorescence chromatography and belongs to the field of a water environment monitoring technology or an agricultural monitoring technology. Accordingto the method, a sample is filtered, enriched, eluted and concentrated to obtain a constant volume based on the characteristics that fluorescent light can be stimulated by the microcystin-LR under theultraviolet irradiation; rapid, accurate and efficient detection of the microcystin-LR in a water body is realized by adopting the high performance liquid fluorescence chromatography; the method hasguiding significance on monitoring, management and protection of a water body pollution condition.

Description

1. Technical field [0001] The invention belongs to the field of water environment monitoring technology or agricultural monitoring technology, and in particular relates to a high-efficiency liquid phase fluorescence chromatography detection method for microcystin-LR in water bodies. 2. Background technology [0002] Microcystins (MCs) are a class of cyclic heptapeptide toxin metabolites produced by the hydration of Microcystis aeruginosa, Anabaena blooms, Oscillating algae, etc. in the cyanobacteria. More than 90 species have been discovered so far. Composed of two isomers, it has strong carcinogenicity. Long-term drinking of water rich in high concentration of MCs may induce liver cancer. MCs can also be bioaccumulated through the food chain, causing damage to the human body, and has persistent biological pollution. Among them, microcystins MC-LR (L represents leucine, R represents arginine) is the most toxic and widely distributed configuration. With the increasing pollut...

AI Technical Summary

Problems solved by technology

These detection methods all use the physical and chemical properties of MCs combined with sensitive photoelectric technology for analysis and determination. Although good measurement results have been achieved, there are disadvantages such as high operating cost of equipment, complicated and time-consuming pretreatment, and low recovery rate.

Immunoassay uses the specific recognition principle of antibodies to antigens to detect various toxins. The method has high sensitivity but low specificity. Antibodies and toxins are prone to cross-reactions, sometimes false positive reactions occur, and the identification of toxin types is poor. , with limited application

Bioassays usually use oral gavage or intraperitoneal injection of mice to evaluate the toxicity of MCs. This method is simple and intuitive to operate, but it cannot distinguish the isomers of the toxin, and the quantification is not accurate enough and the workload is heavy.

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