Deer species source identification primer pair for cornua cervi pantotrichum products and identification method of deer species source identification primer pair

Abstract

The invention discloses a deer species source identification primer pair for cornua cervi pantotrichum products. The identification primer pair comprises a deer identification primer pair DcF-DcR anda cornua cervi pantotrichum identification primer pair Rdfp-Rdrp. The invention also provides an identification method of the deer species source identification primer pair for the cornua cervi pantotrichum products. The method comprises the following steps: S1, preparing a preservation solution of a sample; S2, extracting DNA; S3, identifying the tissue sample by a deer identification program; and S4, determining the sequence after identification by the identification program of cornua cervi pantotrichum to judge the specific species of the cornua cervi pantotrichum product. The identification primer pair has the beneficial effects that integrated and accurate identification of deer and cornua cervi pantotrichum products is achieved by the deer identification primer pair and the cornua cervi pantotrichum identification primer pair; by big data analysis of deer genome sequence, identification accuracy of the cornua cervi pantotrichum product is improved; by first deer identification and then the cornua cervi pantotrichum identification program, accurate identification is achieved; and multiple groups of controls are set, the purified and identified products are recovered by gel extraction, and sequencing and analyzing are performed, so that accurate identification is achieved.

Description

technical field [0001] The invention relates to the field of detection and identification of velvet products, in particular to a pair of primers for identifying deer species sources of velvet products and an identification method thereof. Background technique [0002] Chinese Pharmacopoeia stipulates that sika deer and red deer antler are traditional precious medicinal materials. The main product forms of velvet antler circulating in the Chinese herbal medicine market are slices and powders, making it difficult to distinguish velvet with the naked eye. In addition, the prices of antlers from different sources of deer species (such as: sika deer, red deer, red deer and reindeer) vary greatly. Due to the drive of profit, a large number of New Zealand red deer, North American and Nordic reindeer antlers flow into our country from abroad, imitating traditional Chinese medicine sika deer And red deer antler slices (powder). [0003] In order to distinguish the sika deer, red de...

AI Technical Summary

Problems solved by technology

The main forms of velvet antler circulating in the Chinese herbal medicine market are slices and powders, making it difficult to distinguish velvet with the naked eye

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