Lipoprotein (a) detection kit
A technology for detecting kits and lipoproteins, applied in biological testing, material inspection products, etc., can solve problems such as easy interference of detection values, false positive measurement values, and long detection time, so as to avoid false positive measurement values and be accurate The effect of high reliability and low production cost
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Embodiment 1
[0025] Preparation and determination method of lipoprotein (a) detection kit
[0026] The kit of the present invention relates to the main raw materials of reagents as follows:
[0027] 1. The goat anti-human apolipoprotein (a) polyclonal antibody made by our company according to the conventional method, after affinity purification, the titer is 1:100000 by indirect ELISA method, which meets the requirements of this reagent. The antibody has good specificity and no cross-reaction with other antigens.
[0028] 2. In the present invention, only polystyrene latex particles with carboxyl groups are used for experiments, and the corresponding detection wavelength is 600nm.
[0029] 3. Recombinant apolipoprotein (a) is self-made by our company, expressed in Escherichia coli or yeast, and purified by affinity and ion exchange. The apolipoprotein (a) has high purity and good stability, and is used as the standard product of this reagent .
[0030] The main reagents and reference ...
Embodiment 2
[0036] Lipoprotein (a) detection kit was tested on various performance indicators on Hitachi 7060
[0037] 1. High value linear determination
[0038] The recombinant apolipoprotein (a) was added to the blank serum, and the final concentration of the apolipoprotein (a) in the serum was 1000 mg / l. Serum was diluted with 0.9% NaCl to obtain 5 serum samples containing different concentrations of apolipoprotein (a). Theoretical concentration of apolipoprotein (a) is 62.5mg / L, 125mg / L, 250mg / L, 500mg / L, 1000mg / L respectively. The blank serum was used as a sample with apolipoprotein (a) concentration of 0. Each sample is measured 3 times with the lipoprotein (a) kit among the present invention, and average value is obtained. Correlation analysis is carried out to measured value, as can be seen from Table 1, the highest detection range of the detection kit of the present invention can reach 1000mg / L, see figure 2 , Y-axis measured value, X-axis theoretical value (apolipoprotein ...
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