Chemiluminescence enzyme-linked immunoassay detection kit for Lp-PLA2 (lipoprotein-associated phospholipaseA2)

Abstract

The invention discloses a chemiluminescence enzyme-linked immunoassay detection kit for Lp-PLA2 (lipoprotein-associated phospholipaseA2). The kit is characterized by comprising a reagent R1 and a reagent R2, wherein the reagent R1 is a 0.25M ammonium chloride buffer solution containing 5 g / L polyethylene glycol 8000, 10 g / L sodium chloride, 4 g / L EDTA (ethylene diamine tetraacetic acid), 8 g / L saccharose, 2 g / L bovine serum albumin, 5 g / L Tween 20 and 1 g / L sodium azide; the reagent R2 is a 0.1M ammonium chloride buffer solution containing a polystyrene latex particle mixture coated with anti-human apolipoprotein polyclonal antibodies and prepared with a chemical cross-linking method, a 3 g / L HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) buffer solution, aspartame and 2 g / L sodium azide, wherein the weight-to-volume ratio of aspartame to the reagent R2 is 0.2: 100 g / L, and the pH value is 9.0. The detection kit has high detection sensitivity, high accuracy, good precision, good linearity within the detection range, good stability, low production cost and low blank absorbency, and also has an anti-interference property.

Description

technical field [0001] The present invention relates to kits, in particular to a lipoprotein-associated phospholipase A2 (Lp-PLA2) chemiluminescence ELISA detection kit Background technique [0002] Lipoprotein-associated phospholipase A2 (Lp-PLA2) is a phospholipase A2 superfamily closely related to arteriosclerosis and ischemic cardiovascular and cerebrovascular diseases that has attracted widespread attention in recent years, and a new inflammatory marker substance and an independent risk factor. Its molecular weight is 45KD, synthesized and secreted by mature macrophages and lymphocytes, and regulated by inflammatory mediators. Lipoprotein phospholipase A2 has a pro-inflammatory effect, so the production and release of Lp-PLA2 from inflammatory cells can also be interpreted as an excellent indicator of pro-inflammatory response. Lp-PLA2 produces oxidized molecules in blood vessel walls that are more prone to atherosclerosis and unstable plaques. Elevated levels of Lp-...

AI Technical Summary

Problems solved by technology

[0003] At present, known methods for detecting the concentration of lipoprotein (a) include enzyme-linked immunoassay, radioimmunoassay, fluorescent immunoassay and other detection methods. The operation of the above-mentioned determination methods is relatively complicated, the detection takes a long time, and the shortcomings of excessive waste of resources , not suitable for routine testing

A lipoprotein (a) detection kit is disclosed on the website of the State Intellectual Property Office of China. It adopts latex-enhanced immunoturbidimetry, which has the advantages of good stability and convenient detection. However, the rheumatoid factor contained in human serum is harmful to the The detection value of the kit is prone to interference, which makes the measured value of individual serum with high content of rheumatoid factor appear false positive

In addition, the existing latex-enhanced immunoturbidimetric method uses physical adsorption to label polyclonal antibodies on polystyrene latex particles without carboxyl groups. The polystyrene latex particles without carboxyl groups have poor stability and physical The effect of adsorption is also not good, it is easy to cause the antibody to fall off from the surface of latex particles