Reagent kit for measuring content of C-reactive protein in whole blood and measuring method thereof

The technology of a reactive protein and a determination method is applied to a kit for determining the content of C-reactive protein in whole blood and the field of determination thereof, and achieves the effects of simple operation, reduced pain and accurate results.

Inactive Publication Date: 2010-12-08
武汉中太生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] In order to solve the problem that the determination of CRP content in the prior art uses serum and plasma as detection samples, which is not suitable for children, especially neonates and patients with extensive burns, and other subjects who h

Method used

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  • Reagent kit for measuring content of C-reactive protein in whole blood and measuring method thereof
  • Reagent kit for measuring content of C-reactive protein in whole blood and measuring method thereof

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0032] The C-reactive protein content determination kit in whole blood of the present invention comprises reagent 1 and reagent 2, and reagent 1 comprises surfactant triton 0.1g / L, stabilizer bovine serum albumin 0.5g / L, preservative 0.05 g / L and HEPES buffer (100mmol / L, PH7.4); Reagent 2 includes 0.5g / L polystyrene latex adsorbed with CRP antibody, stabilizer egg albumin 0.5g / L, preservative 0.05g / L and PH7.4 HEPES buffer 100mmol / L.

[0033] The method for measuring C-reactive protein content in whole blood of the present invention comprises the following steps

[0034] A. Collect the peripheral blood of the subject, which is a whole blood sample;

[0035] B. Incubate 5ul of the whole blood sample with 200ul of the above reagent 1 at 37°C and mix for 30 seconds to dissolve the red blood cells in the sample;

[0036] C. Add 200ul of reagent 2 equilibrated to room temperature to the solution in step B and mix well, so that the C-reactive protein in the sample and the C-react...

Embodiment 2

[0039] The C-reactive protein content determination kit in whole blood of the present invention comprises reagent 1 and reagent 2, and reagent 1 comprises surfactant saponin 3g / L, stabilizer polyamino acid 5g / L, preservative 0.5g / L and HEPES buffer (100mmol / L, PH7.4); Reagent 2 includes polystyrene latex that 3g / L is adsorbed with CRP antibody, stabilizer skimmed milk powder 5g / L, preservative 0.5g / L and HEPES buffer ( 100mmol / L, pH7.4).

[0040] The method for measuring C-reactive protein content in whole blood of the present invention comprises the following steps

[0041] A. Collect the peripheral blood of the subject as a whole blood sample;

[0042] B. Mix 5 ul of the whole blood sample with 200 ul of the above reagent 1 incubated at 37°C for 30 seconds to dissolve the red blood cells in the sample;

[0043] C. Add 200ul of reagent 2 equilibrated to room temperature to the solution in step B and mix well, so that the C-reactive protein in the sample and the C-reactive p...

Embodiment 3

[0046]The C-reactive protein content determination kit in whole blood of the present invention comprises reagent 1 and reagent 2, and reagent 1 comprises surfactant cetylamino ammonium bromide 1g / L, stabilizer bovine serum albumin 1g / L, Stabilizer egg albumin 1g / L, preservative 0.1g / L and HEPES buffer (100mmol / L, pH7.4); Reagent 2 includes 2g / L polystyrene latex adsorbed with CRP antibody, stabilizer cattle Serum albumin 1g / L, preservative 0.1g / L and HEPES buffer (100mmol / L, PH7.4).

[0047] The method for measuring C-reactive protein content in whole blood of the present invention comprises the following steps

[0048] A. Collect the peripheral blood of the subject as a whole blood sample;

[0049] B. Incubate 5ul of the whole blood sample with 200ul of the above reagent 1 at 37°C and mix for 30 seconds to dissolve the red blood cells in the sample;

[0050] C. Add 200ul of reagent 2 equilibrated to room temperature to the solution in step B and mix well, so that the C-reac...

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Abstract

The invention discloses a reagent kit for measuring the content of C-reactive protein in whole blood and a measuring method thereof. The reagent kit comprises a reagent 1 and a reagent 2, wherein the reagent 1 comprises a surfactant and a proper amount of buffer solution, and the content of the surfactant is 0.1-3g/L; and the reagent 2 comprises polystyrene latex adsorbed with a C-reactive protein antibody and a proper amount of buffer solution, and the content of the polystyrene latex adsorbed with the C-reactive protein antibody is 0.5-3g/L. The measuring method comprises the following steps of: collecting a whole blood sample of a subject, uniformly mixing the reagent 1 and the whole blood sample, then adding the reagent 2 for uniformly mixing, detecting the absorbance and the scattering light intensity of a reaction system at 625-800nm, and computing the content of the C-reactive protein in the whole blood by contrasting a standard curve. In the reagent kit and the measuring method, the whole blood of the subject can be used as a measured sample, the operation is simple, and results are accurate. The invention relieves the pain of the subjects, particularly for the child and newborn subjects and the subjects with more difficult vein blood collection, such as patients with large-area burns, and the like.

Description

technical field [0001] The invention relates to a test kit and a test method for measuring the content of reactive protein in blood, in particular to a test kit for measuring the content of C-reactive protein in whole blood and a test method thereof Background technique [0002] C-reactive protein was discovered by Tillet and Francis in 1930. Initially, they observed that the serum of some acute patients could react with the capsular C polysaccharide of Streptococcus pneumoniae, and then confirmed that the substance that could react with C polysaccharide was a protein, so they named this protein C-reactive protein (C-reaction protein). protein, CRP). It has been confirmed that CRP is part of the body's non-specific immune mechanism, it binds C polysaccharides, and in Ca 2+ When present, it can bind to phosphorylcholine on the cell membrane, activate the classical pathway of complement, enhance the phagocytosis of leukocytes, regulate the function of lymphocytes or monocyte...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/545G01N21/31
Inventor 马全新
Owner 武汉中太生物技术有限公司
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