Preparation method for polystyrene latex with carboxyl on surface and for immunoassay technology

A technology of polystyrene latex and immunoassay, which is applied in the direction of measuring devices, instruments, and analytical materials, etc., can solve the problems of unsatisfactory particle size uniformity of latex particles, affecting the performance of latex, and affecting the accuracy of measurement, etc., to achieve surface Clean, good measurement effect, uniform particle size distribution effect

Active Publication Date: 2010-07-14
NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, in the conventional latex preparation process, emulsifiers are mostly used as auxiliary raw materials, but after the latex is prepared, a certain amount of emulsifiers often remain in it, and the existence of emulsifiers will affect the final performance of the latex
[0005] In addition, the wavelength used for latex-enhanced immune turbidimetry is related to the particle size of the latex used, and the particle size uniformity of the latex particles prepared by the prior art

Method used

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  • Preparation method for polystyrene latex with carboxyl on surface and for immunoassay technology
  • Preparation method for polystyrene latex with carboxyl on surface and for immunoassay technology
  • Preparation method for polystyrene latex with carboxyl on surface and for immunoassay technology

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Embodiment 1

[0030] (1) Add styrene, methacrylic acid, and water to a three-necked flask and stir for 10 minutes; the above-mentioned styrene is 0.041mol, methacrylic acid is 8.7mmol, and water is 10ml;

[0031] (2) After the reaction system stirring of step (1) gained finishes, in the reaction system, pass into the N of 30min 2 , then about 0.066g of potassium persulfate and about 0.207g of sodium chloride were added to 40ml of water to configure an aqueous solution, and the above-mentioned aqueous solution was added to the reaction system. After the addition, the concentration of NaCl in the reaction system was 0.064mol / The concentration of L and KPS is 4.38×10 -3 mol / L (total water consumption is the water that step (1) adds and step (2) configures the used water of sodium chloride and potassium persulfate to be 50ml, configures the used water of NaCl and KPS aqueous solution just to ensure that NaCl and KPS Completely dissolve in water, because if NaCl and KPS are added to the reacti...

Embodiment 2

[0038] (1) Add styrene, methacrylic acid, and water to a three-necked flask and stir for 10 minutes; the above-mentioned styrene is 0.041mol, methacrylic acid is 8.7mmol, and water is 10ml;

[0039] (2) After the reaction system stirring of step (1) gained finishes, in the reaction system, pass into the N of 30min 2, then about 0.117g of potassium persulfate and about 0.207g of sodium chloride are added to 40ml of water to configure an aqueous solution, and the above-mentioned aqueous solution is added to the reaction system. After the addition, the concentration of NaCl in the reaction system is 0.064mol / The concentration of L and KPS is 7.88×10 -3 mol / L, and then continue to pass through 20min of N 2 , in the whole reaction system, the total amount of the added water is 50ml, and now the concentration of the methacrylic acid added in step (1) in the total water consumption is 0.174mol / L;

[0040] (3) After the nitrogen feed is completed, the reaction system obtained in st...

Embodiment 3

[0044] (1) Add styrene, methacrylic acid, and water to a three-necked flask and stir for 10 minutes; the above-mentioned styrene is 0.041mol, methacrylic acid is 8.7mmol, and water is 10ml;

[0045] (2) After the reaction system stirring of step (1) gained finishes, in the reaction system, pass into the N of 30min 2 , and then about 0.12g of potassium persulfate and about 0.207g of sodium chloride are added to 40ml of water to configure an aqueous solution, and the above-mentioned aqueous solution is added to the reaction system. After the addition, the concentration of NaCl in the reaction system is 0.064mol / The concentration of L and KPS is 8.14×10 -3 mol / L, and then continue to pass through 20min of N 2 , in the whole reaction system, the amount of water added is 50mL, and now the concentration of the methacrylic acid added in step (1) in the total water consumption is 0.174mol / L;

[0046] (3) After the nitrogen feed is completed, the reaction system obtained in step (2)...

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Abstract

The invention discloses a preparation method for polystyrene latex with carboxyl on the surface and for immunoassay technology; the preparation method comprises the following steps that: styrene, methyl methacrylate and water are added into a three-neck flask to be stirred for 10 to 30min; the molar ratio of the methyl methacrylate to the styrene is 0.1 to 0.2:1; after stirring, N2 is fed into the reaction system; and then potassium persulfate and the aqueous solution of sodium chloride are added in, so that the concentration of sodium chloride in the reaction system is 0.064mol/L, and the concentration of the potassium persulfate is 7.00*10-4 to 25.0*10-3mol/L; N2 is continued to be fed in; the temperature goes up to 60 to 80DEG C, and the reaction is carried out for 12 to 24h; and after the reaction, the temperature is cooled to room temperature, and the target product of the invention is prepared. The latex prepared by the invention has the advantages of uniform grain size, good monodispersity and strong stability; and simultaneously, the surface of the latex has higher carboxyl content, and the preparation method is applicable to a latex immune turbidity determination method.

Description

technical field [0001] The invention relates to a preparation method of polystyrene latex with carboxyl groups on the surface used in immunoassay technology. Background technique [0002] Generally, in the field of immunoassays, the antigen-antibody reaction is first performed to form an immune complex, and then quantified by turbidimetric method, but it takes a long time to form the antigen-antibody complex. Latex Enhanced Immunoturbidimetric Assay (LETIA for short) is a new immunoassay technique that has emerged in the field of immunoassays in recent years. LETIA does not require special instruments, and can be measured by automatic or semi-automatic biochemical analyzers, and is suitable for popularization in basic medical units application. At present, it has been applied to the quantitative determination of many analytes, such as ASO, RF, CRP, transferrin, toxoplasma, rubella, β 2 - Microglobulin and drug monitoring, etc. [0003] The principle of LETIA is that the l...

Claims

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Application Information

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IPC IPC(8): C08F212/08C08F220/06C08F2/22G01N33/531
Inventor 邹炳德沈红燕
Owner NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO LTD
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