Preparation method for polystyrene latex with carboxyl on surface and for immunoassay technology

Abstract

The invention discloses a preparation method for polystyrene latex with carboxyl on the surface and for immunoassay technology; the preparation method comprises the following steps that: styrene, methyl methacrylate and water are added into a three-neck flask to be stirred for 10 to 30min; the molar ratio of the methyl methacrylate to the styrene is 0.1 to 0.2:1; after stirring, N2 is fed into the reaction system; and then potassium persulfate and the aqueous solution of sodium chloride are added in, so that the concentration of sodium chloride in the reaction system is 0.064mol / L, and the concentration of the potassium persulfate is 7.00*10-4 to 25.0*10-3mol / L; N2 is continued to be fed in; the temperature goes up to 60 to 80DEG C, and the reaction is carried out for 12 to 24h; and after the reaction, the temperature is cooled to room temperature, and the target product of the invention is prepared. The latex prepared by the invention has the advantages of uniform grain size, good monodispersity and strong stability; and simultaneously, the surface of the latex has higher carboxyl content, and the preparation method is applicable to a latex immune turbidity determination method.

Description

technical field [0001] The invention relates to a preparation method of polystyrene latex with carboxyl groups on the surface used in immunoassay technology. Background technique [0002] Generally, in the field of immunoassays, the antigen-antibody reaction is first performed to form an immune complex, and then quantified by turbidimetric method, but it takes a long time to form the antigen-antibody complex. Latex Enhanced Immunoturbidimetric Assay (LETIA for short) is a new immunoassay technique that has emerged in the field of immunoassays in recent years. LETIA does not require special instruments, and can be measured by automatic or semi-automatic biochemical analyzers, and is suitable for popularization in basic medical units application. At present, it has been applied to the quantitative determination of many analytes, such as ASO, RF, CRP, transferrin, toxoplasma, rubella, β 2 - Microglobulin and drug monitoring, etc. [0003] The principle of LETIA is that the l...

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Problems solved by technology

[0004] At present, in the conventional latex preparation process, emulsifiers are mostly used as auxiliary raw materials, but after the latex is prepared, a certain amount of emulsifiers often remain in it, and the existence of emulsifiers will affect the final performance of the latex

[0005] In addition, the wavelength used for latex-enhanced immune turbidimetry is related to the particle size of the latex used, and the particle size uniformity of the latex particles prepared by the prior art is not very ideal, so it will affect the accuracy of the measurement.

[0006] According to research, the polystyrene latex with carboxyl group modification on the surface is polystyrene as the core, and the carboxyl group on its surface can be coupled to antigen or antibody by chemical method. This latex is suitable for latex-enhanced immune turbidity assay However, there are few reports on the preparation of polystyrene latex with high carboxyl content and uniform particle size on the surface

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