Quick antibacterial test method and kit

A test method and kit technology, applied in the field of microbiology test, can solve the problems of long test period, impact on quantification, poor control of the number of colonies in the negative control group, etc., and achieve the effect of saving test cost, less reagent volume and cost

Active Publication Date: 2015-01-21
承功(厦门)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The test period of this standard method is relatively long, about 48 hours, and the number of colonies in the negative control group (without antibacterial agent) is not easy to control. Generally, the number of colonies in each negative control group is better between 100-900, too lo

Method used

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  • Quick antibacterial test method and kit
  • Quick antibacterial test method and kit
  • Quick antibacterial test method and kit

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0039] Example 1

[0040] Such as figure 1 , figure 2 As shown, a rapid antibacterial test kit includes:

[0041] (1) Sterile multi-well test plate 1 (15 holes): It is made of transparent plastic mold and contains 15 test holes of equal size in 3 rows and 5 columns. The left three columns are sample holes, and the right two columns are control holes. The volume of each detection hole 2 is 0.1-0.5ml, and the bottom of the detection hole has the same thickness and uniform texture.

[0042] (2) Moisturizing and heat preservation culture box (1): It is made of transparent plastic material, composed of a box body and a lid, with a sterile wet tissue at the bottom, and a detection plate 1 on the sterile wet tissue.

[0043] (3) Culture medium (30ml): a bottle of LB culture medium.

[0044] (4) Test bacteria (1 bottle): Escherichia coli (ATCC 25922) grows on the surface of the inclined LB agar medium.

[0045] (5) Positive control antibacterial agent (1ml): 0.1% chlorhexidine digluconate.

Example Embodiment

[0046] Example 2

[0047] A preparation method of a rapid antibacterial test kit includes the following steps:

[0048] (1) Prepare a sterile multi-well detection plate 1: Press figure 2 The mold shown is then made using a transparent plastic mold in a known manner. The detection plate 1 consists of 15 detection holes 2 in 3 rows and 5 columns. The 3X3 holes on the left (S1, S2, and S3 groups) are sample holes for detecting antibacterial samples; the right part is 2X3 holes (D1, D2 in two groups). ) Is a control hole, used to detect positive control and negative control antibacterial agents; the volume of each hole is about 0.35ml; sealed and packaged with a sterile plastic bag for use.

[0049] (2) Preparation of culture solution: The LB culture solution is prepared and divided into 50ml glass bottles, 30ml per bottle, sealed and stored after autoclaving for later use.

[0050] (3) Preparation of test bacteria: After autoclaving the LB agar medium, cool it to about 50°C, and put it...

Example Embodiment

[0054] Example 3

[0055] Compare the effects of OD470 and OD600 in detecting bacterial density:

[0056] Take 0.5ml of Escherichia coli (ATCC 25922) overnight bacterial solution, dilute it with 5ml of LB broth and shake culture for 3h on a 37°C shaker. Take 0.1ml of this bacterial broth and mix with 0.1ml of LB broth, and then use LB The culture broth was diluted 7 times, and only the LB broth did not contain bacteria at the last time. Then transfer the serially diluted bacterial broth (8 parts) to the enzyme plate, and detect at 470nm and 600nm with a microplate reader. The OD value of each group of bacterial solution after gradient dilution, the results are shown in Table 1 and image 3 Shown.

[0057] Table 1. Detecting OD450 and OD600 results under different dilutions of bacterial liquid

[0058] Bacteria density

[0059] The test results prove that both 470nm wavelength (OD470nm) and 600nm wavelength (OD600nm) can be used to determine the density of bacteria in the bacteria...

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Abstract

The invention provides a quick antibacterial test method and a kit. The kit comprises an aseptic porous detection board, a moisturizing and heat preservation culture box, a culture solution, test organisms and a positive control antibacterial agent, wherein the aseptic porous detection board comprises three or more detection holes with the same size, transparent materials with the same thicknesses and uniform texture are arranged at the bottoms of the detection holes, the moisturizing and heat preservation culture box is composed of a box body and a box cover, 1-10 detection boards can be placed in the box body, the culture solution is a culture solution necessary for the growth of the test organisms. The quick antibacterial test method and the kit provided by the invention can be used for quickly and conveniently detecting the antibacterial ability of a test sample and have the advantages of high throughput and low cost.

Description

technical field [0001] The invention belongs to the field of microbial tests, in particular to a rapid antibacterial test method and a kit. Background technique [0002] Antibacterial test is a test method used to detect and evaluate the antibacterial effect of antibacterial agents. It can also be used to screen new antibacterial drugs and detect bacterial resistance. [0003] The current standard antibacterial test method is based on the calculation of the number of colonies on the agar plate to determine the performance of the antibacterial agent, and the test period is relatively long. The operation steps of the standard anti-bacteria product anti-bacteria performance test method are as follows: [0004] Culture the test bacteria (Staphylococcus aureus: ATCC 6538 or Escherichia coli: 8099 or ATCC 25922) on a slant for 24 hours, wash the culture with PBS, and make a bacterial suspension. The required concentration is: drop 100 μl on 5ml control sample In the liquid (50X ...

Claims

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Application Information

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IPC IPC(8): C12M1/34C12Q1/18
CPCC12M23/12C12M37/06C12Q1/18
Inventor 邹潮
Owner 承功(厦门)生物科技有限公司
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