Quick antibacterial test method and kit
A test method and kit technology, applied in the field of microbiology test, can solve the problems of long test period, impact on quantification, poor control of the number of colonies in the negative control group, etc., and achieve the effect of saving test cost, less reagent volume and cost
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Embodiment 1
[0040] Such as figure 1 , figure 2 Shown, a rapid antibacterial test kit, comprising:
[0041] (1) Sterile porous detection plate 1 (15 wells): made of transparent plastic molding, containing 3 rows and 5 columns of 15 detection holes 2 of equal size, the three columns on the left are sample wells, and the two columns on the right are control wells. The volume of each detection hole 2 is 0.1-0.5ml, and the bottom of the detection hole has uniform thickness and uniform texture.
[0042] (2) Moisturizing and heat-preserving culture box (1): It is made of transparent plastic, consisting of a box body and a box cover. A sterile wet tissue is placed on the bottom, and the detection plate 1 is placed on the sterile wet tissue.
[0043] (3) Culture solution (30ml): a bottle of LB culture solution.
[0044] (4) Test bacteria (1 bottle): Escherichia coli (ATCC 25922) grows on the surface of the LB agar medium on the slope.
[0045] (5) Positive control antibacterial agent (1 ml): ...
Embodiment 2
[0047] A preparation method of a rapid antibacterial test kit, comprising the steps of:
[0048] (1) Preparation of sterile multi-well test plate 1: press figure 2 Molding as shown is then made with transparent plastic casting by known methods. The test plate 1 consists of 15 test holes 2 in 3 rows and 5 columns. The 3X3 holes on the left (three groups of S1, S2 and S3) are sample holes for detecting antibacterial samples; the 2X3 holes on the right (two groups of D1 and D2) ) is a control well, which is used to detect positive control and negative control antibacterial agents; the volume of each well is about 0.35ml; it is sealed and packaged in a sterile plastic bag for subsequent use.
[0049] (2) Preparation of culture solution: LB culture solution was prepared and divided into 50ml glass bottles, 30ml per bottle, sealed and stored after autoclaving, for future use.
[0050](3) Preparation of test bacteria: after high-pressure sterilization of LB agar medium, cool it to...
Embodiment 3
[0055] Compare the effect of OD470 and OD600 in detecting bacterial density:
[0056] Take 0.5ml of Escherichia coli (ATCC 25922) overnight bacteria solution, dilute it with 5ml of LB culture solution and culture it on a shaker at 37°C for 3 hours, take 0.1ml of the bacteria solution and mix it with 0.1ml of LB culture solution, and then use LB The culture solution was serially diluted 7 times, and the last time only the LB culture solution was free of bacteria, and then the serial serial dilution of the bacteria solution (8 parts) was transferred to the microplate plate, and detected at 470nm and 600nm respectively with a microplate reader The OD value of each group of bacteria solution after serial dilution, the results are shown in Table 1 and image 3 shown.
[0057] Table 1. The results of detection of OD450 and OD600 at different bacterial dilutions
[0058] Bacteria density
[0059] The test results prove that the bacterial density in the bacterial liquid ca...
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