Fast visual detection method of organophosphorus type pesticide

A detection method, organophosphorus technology, applied in the field of analysis and detection, can solve the problems of limited detection sensitivity and application range, expensive instruments, false negative and false positive results, etc., to improve small size and surface effect, reduce catalytic activity, and enhance specificity sexual effect

Active Publication Date: 2019-04-23
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
View PDF7 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the detection and analysis technology of organophosphorus pesticides requires large-scale instrument detection, such as liquid chromatography-mass spectrometry (LC-MS), gas chromatography-mass spectrometry (GC-MS), etc.; although such methods can accurately determine the concentration of organophosphorus pesticides, However, there are disadvantages such as time-consuming detection, expensive equipment, professional staff, and on-site rapid screening; highly specific enzyme activity inhibition methods, such as acetylcholinesterase or butyrylcholinesterase, can be eliminated by org

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Fast visual detection method of organophosphorus type pesticide
  • Fast visual detection method of organophosphorus type pesticide
  • Fast visual detection method of organophosphorus type pesticide

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0045] Example 1

[0046] A rapid visual detection method for organophosphorus pesticides includes the following steps:

[0047] 1) Combine 9.5 mL of DTAB with a concentration of 0.02 mol / L and 500 μL with a concentration of 5×10 -5 After mixing the mol / L ZnTPyP N,N-dimethylformamide solution, perform ultrasonic dispersion (100W, 25°C) for 10-15min, and in a water bath at 70°C for 15-20min, to obtain nanoporphyrin 2;

[0048] 2) Change the concentration of 100μL to 5×10 -7 mol / L nanoporphyrin 2 aqueous solution, add to 100μL of N,N-dimethylformamide solution with a concentration of 3mmol / L TMB, 100μL of H with a mass fraction of 30% 2 O 2 In the mixed solution I formed by 700μL acetate buffer solution (0.2mol / L, pH=3.6), fully shake the reaction for 10min, measure its absorbance value at 652nm in the ultraviolet-visible spectrum and record it as A 0 ; Another precision draw 100μL concentration is 5×10 -7 mol / L Nanoporphyrin 2 aqueous solution was added to 100μL of N,N-dimethylformamid...

Example Embodiment

[0050] Example 2

[0051] A rapid visual detection method for organophosphorus pesticides includes the following steps:

[0052] 1) Combine 9.5 mL of DTAB solution with a concentration of 0.05 mol / L and 500 μL with a concentration of 5×10 -5 After mixing the N,N-dimethylformamide solution of mol / L ZnTPyP, perform ultrasonic dispersion (100W, 25°C) for 10-15min, and at 70°C in a water bath for 15-20min to obtain nanoporphyrin 3;

[0053] 2) Change 100μL concentration to 1×10 -6 mol / L nanoporphyrin 3 aqueous solution, add 100μL of N,N-dimethylformamide solution with a concentration of 3mmol / L TMB, 100μL of H with a mass fraction of 30% 2 O 2 In the mixed solution I formed by 700μL acetate buffer solution (0.2mol / L, pH=3.6), fully shake the reaction for 10min, measure its absorbance value at 652nm in the ultraviolet-visible spectrum and record it as A 0 ; Another precision draw 100μL concentration is 1×10 -6 mol / L Nanoporphyrin 3 aqueous solution was added to 100μL of N,N-dimethylformami...

Example Embodiment

[0055] Example 3

[0056] A rapid visual detection method for organophosphorus pesticides (trichlorfon), including the following steps:

[0057] 1) Add 100μL to 700μL acetate buffer solution (0.2mol / L, pH=3.6) to a concentration of 5×10 -7 mol / L DTAB-ZnTPyP (nanoporphyrin 2 obtained in Example 1) aqueous solution, 100 μL of TMB N,N-dimethylformamide solution with a concentration of 3 mmol / L, and 100 μL of H with a mass fraction of 30% 2 O 2 The solution was fully shaken and reacted for 10 minutes, and the absorbance value of the obtained mixed solution I at 652nm in the ultraviolet-visible spectrum was measured and recorded as A 0 ;

[0058] 2) Add 100μL to 600μL acetate buffer solution (0.2mol / L, pH=3.6) to a concentration of 5×10 -7 mol / L DTAB-ZnTPyP aqueous solution, 100 μL TMB N,N-dimethylformamide solution with a concentration of 3 mmol / L, and 100 μL H with a mass fraction of 30% 2 O 2 Then add 100μL of trichlorfon aqueous solution of different concentrations (respectively 5, 20,...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Concentrationaaaaaaaaaa
Login to view more

Abstract

The invention provides a fast visual detection method of organophosphorus type pesticide. Firstly, ZnTPyP is subjected to hydrothermal self-assembly by a cationic surface active agent for preparing nanometer porphyrin; then, the nanometer porphyrin is used for the fast visual detection on organophosphorus. On the basis of biomimetic peroxidase type activity of the nanometer porphyrin, 3,3',5,5'-tetramethyl benzidine is catalyzed and oxidized; the characteristic absorption in a 652nm position of the ultraviolet-visible spectrum occurs; colorless to blue chromogenic reaction is generated; specific reaction of the organophosphorus type pesticide and the nanometer porphyrin is utilized; the catalytic activity of the nanometer porphyrin is reduced; the generation of blue oxidation product oxTMBis reduced; the reduction of the absorbancy in the 652nm position of the ultraviolet-visible spectrum is shown; the reduction degree and the concentration of the organophosphorus type pesticide are in positive correlation relationship; the detection of the organophosphorus type pesticide is realized; the operation of the detection method is simple and convenient; the reagent toxicity is small; the cost is low; the sensitivity is high; the stability is high; the applicability is high.

Description

technical field [0001] The invention belongs to the technical field of analysis and detection, and in particular relates to a rapid and visual detection method for organophosphorus pesticides. Background technique [0002] Organophosphorus pesticides are widely used as insecticides in the process of agricultural production because of their wide range of targets and low price. A large amount of organic phosphorus widely exists in food, water and soil, which poses a great threat to human health, and also seriously affects the ecosystem and homeland security. Organophosphorus pesticides left on crops or fruit thins irreversibly inhibit the activity of acetylcholinesterase (AChE) in the human body, leading to the accumulation of the neurotransmitter acetylcholine in the body, eventually leading to organ failure and death. Therefore, the detection of organophosphorus pesticides has become an urgent problem to be solved. [0003] At present, the detection and analysis technology...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N21/78G01N21/31G01N1/38
CPCG01N1/38G01N21/3103G01N21/78
Inventor 付海燕时琼佘远斌
Owner SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap