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Fast visual detection method of organophosphorus type pesticide

A detection method, organophosphorus technology, applied in the field of analysis and detection, can solve the problems of limited detection sensitivity and application range, expensive instruments, false negative and false positive results, etc., to improve small size and surface effect, reduce catalytic activity, and enhance specificity sexual effect

Active Publication Date: 2019-04-23
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, the detection and analysis technology of organophosphorus pesticides requires large-scale instrument detection, such as liquid chromatography-mass spectrometry (LC-MS), gas chromatography-mass spectrometry (GC-MS), etc.; although such methods can accurately determine the concentration of organophosphorus pesticides, However, there are disadvantages such as time-consuming detection, expensive equipment, professional staff, and on-site rapid screening; highly specific enzyme activity inhibition methods, such as acetylcholinesterase or butyrylcholinesterase, can be eliminated by organophosphorus pesticides Inhibition, so as to realize the detection of organophosphorus, but it is also severely limited by the instability of the enzyme (temperature, pH, etc.), low enzyme yield, high price, and prone to false negative and false positive results.
In addition, although there are a series of techniques such as optical detection analysis and electrochemical method for rapid analysis of organophosphorus pesticides, these detection techniques usually require complex and expensive optical imaging equipment and complex image software, and are also not suitable for rapid on-site application

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  • Fast visual detection method of organophosphorus type pesticide

Examples

Experimental program
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Effect test

Embodiment 1

[0046] A rapid visual detection method for organophosphorus pesticides, comprising the steps of:

[0047] 1) Mix 9.5 mL of an aqueous solution of DTAB with a concentration of 0.02 mol / L and 500 μL of a concentration of 5×10 -5 The mol / L ZnTPyP N,N-dimethylformamide solution is mixed, then ultrasonically dispersed (100W, 25°C) for 10-15min, and placed in a water bath at 70°C for 15-20min to obtain nanoporphyrin 2;

[0048] 2) Dilute 100 μL to a concentration of 5×10 -7 mol / L nano-porphyrin 2 aqueous solution, add to 100 μL of N,N-dimethylformamide solution with a concentration of 3 mmol / L TMB, 100 μL of 30% H 2 o 2 solution, 700 μL acetate buffer solution (0.2mol / L, pH=3.6) in the mixed solution I formed, fully shake the reaction for 10min, measure its absorbance value at 652nm in the ultraviolet-visible spectrum and record it as A 0 ; Another precision pipetting 100μL concentration is 5×10 -7 The mol / L nanoporphyrin 2 aqueous solution was added to 100 μL of N,N-dimethylfor...

Embodiment 2

[0051] A rapid visual detection method for organophosphorus pesticides, comprising the steps of:

[0052] 1) Mix 9.5 mL of an aqueous solution of DTAB with a concentration of 0.05 mol / L and 500 μL of a concentration of 5×10 -5 The mol / L ZnTPyP N,N-dimethylformamide solution is mixed and then ultrasonically dispersed (100W, 25°C) for 10-15min, and then placed in a water bath at 70°C for 15-20min to obtain nanoporphyrin 3;

[0053] 2) Dilute 100 μL to a concentration of 1×10 -6 mol / L nano-porphyrin 3 aqueous solution, add to 100 μL of N,N-dimethylformamide solution with a concentration of 3 mmol / L TMB, 100 μL of 30% H 2 o 2 solution, 700 μL acetate buffer solution (0.2mol / L, pH=3.6) in the mixed solution I formed, fully shake the reaction for 10min, measure its absorbance value at 652nm in the ultraviolet-visible spectrum and record it as A 0 ; Another precise pipetting 100μL concentration is 1×10 -6 The mol / L nanoporphyrin 3 aqueous solution was added to 100 μL of N,N-dimet...

Embodiment 3

[0056] A rapid visual detection method for organophosphorus pesticides (trichlorfon), comprising the steps of:

[0057] 1) Add 100 μL to 700 μL acetate buffer solution (0.2mol / L, pH=3.6) with a concentration of 5×10 -7 mol / L DTAB-ZnTPyP (nano-porphyrin 2 obtained in Example 1) aqueous solution, 100 μL concentration of 3 mmol / L TMB N, N-dimethylformamide solution and 100 μL mass fraction of 30% H 2 o 2 solution, fully shaken and reacted for 10min, and measured the absorbance value of the resulting mixed solution I at 652nm in the UV-visible spectrum as A 0 ;

[0058] 2) Add 100 μL to 600 μL acetate buffer solution (0.2mol / L, pH=3.6) with a concentration of 5×10 -7 mol / L DTAB-ZnTPyP aqueous solution, 100 μL of 3 mmol / L TMB in N,N-dimethylformamide solution and 100 μL of 30% H 2 o 2 solution, and then add 100 μL of trichlorfon aqueous solutions of different concentrations (5, 20, 40, 50, 100, 150, 250, 350 ng / mL respectively), fully shake and react for 10 minutes, and measur...

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Abstract

The invention provides a fast visual detection method of organophosphorus type pesticide. Firstly, ZnTPyP is subjected to hydrothermal self-assembly by a cationic surface active agent for preparing nanometer porphyrin; then, the nanometer porphyrin is used for the fast visual detection on organophosphorus. On the basis of biomimetic peroxidase type activity of the nanometer porphyrin, 3,3',5,5'-tetramethyl benzidine is catalyzed and oxidized; the characteristic absorption in a 652nm position of the ultraviolet-visible spectrum occurs; colorless to blue chromogenic reaction is generated; specific reaction of the organophosphorus type pesticide and the nanometer porphyrin is utilized; the catalytic activity of the nanometer porphyrin is reduced; the generation of blue oxidation product oxTMBis reduced; the reduction of the absorbancy in the 652nm position of the ultraviolet-visible spectrum is shown; the reduction degree and the concentration of the organophosphorus type pesticide are in positive correlation relationship; the detection of the organophosphorus type pesticide is realized; the operation of the detection method is simple and convenient; the reagent toxicity is small; the cost is low; the sensitivity is high; the stability is high; the applicability is high.

Description

technical field [0001] The invention belongs to the technical field of analysis and detection, and in particular relates to a rapid and visual detection method for organophosphorus pesticides. Background technique [0002] Organophosphorus pesticides are widely used as insecticides in the process of agricultural production because of their wide range of targets and low price. A large amount of organic phosphorus widely exists in food, water and soil, which poses a great threat to human health, and also seriously affects the ecosystem and homeland security. Organophosphorus pesticides left on crops or fruit thins irreversibly inhibit the activity of acetylcholinesterase (AChE) in the human body, leading to the accumulation of the neurotransmitter acetylcholine in the body, eventually leading to organ failure and death. Therefore, the detection of organophosphorus pesticides has become an urgent problem to be solved. [0003] At present, the detection and analysis technology...

Claims

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Application Information

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IPC IPC(8): G01N21/78G01N21/31G01N1/38
CPCG01N1/38G01N21/3103G01N21/78
Inventor 付海燕时琼佘远斌
Owner SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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