41 results about "Peroxidase like" patented technology

The invention discloses a boron-doped graphene quantum dot and a preparation method thereof. The boron element-doped graphene quantum dot is prepared by one-step hydrothermal synthesis in a mixed aqueous solution of 1,3,6-trinitropyrene and borax, and the prepared boron-doped graphene quantum dot can emit yellow fluorescence and has good fluorescence stability, and the fluorescence intensity afterthe boron-doped graphene quantum dot is placed for 3 months still keeps 99% of initial fluorescence intensity. The high-stability boron-doped graphene quantum dot provided by the invention has peroxidase-like reaction activity, and can catalyze a color reaction that hydrogen peroxide oxidizes 3,5,3',5'-tetramethylbenzidine. The boron-doped graphene quantum dot has obvious selective recognition ability on iron ions, and the iron ions can quench fluorescent signals of the boron-doped graphene quantum dot, and corresponding stability is achieved within 3 minutes.

A method for improving peroxidase-like activity of nanozyme and a product thereof are disclosed, which relate to the field of artificial enzymes in biochemistry. The method adopts a hydrogen peroxide solution with high concentration to treat the VO₂(B) powder for obtaining a product with high peroxidase-like activity. Compared with the pure VO₂(B) powder, the peroxidase-like activity of the product obtained by the method is increased by 4 to 12 times. The method provided by the present invention adopts raw materials with low cost and mild reaction conditions, is simple in operation and low in cost, which is conducive to batch preparation. The powder product obtained by the method is able to be applied to detect hydrogen peroxide, glucose, etc., and has great application prospects in biosensing, industrial wastewater treatment and sewage treatment.

The invention discloses a preparation method of a copper-gold-platinum composite nanomaterial and application of the copper-gold-platinum composite nanomaterial in detection of cysteine. The preparation method comprises the following steps of adding a copper sulfate solution and a sodium citrate solution in water, adding fresh NaBH4, adding a HAuCl4 water solution after the solution is pale yellow, stirring, then storing at the temperature of 12-16 DEG C to obtain Cu@AuNPs, and placing in a refrigerator to be refrigerated in a dark place for later use; and adding Cu@AuNPs in a K2PtC16 solution, stirring and heating to 80 DEG C, then dropwise adding ascorbic acid, and continuously stirring until the solution is black. The prepared copper-gold-platinum composite nanomaterial has peroxidase sample activity, can enable TMB and a hydrogen peroxide system to be blue, however the addition of the cysteine can inhibit peroxidase sample activity, and the concentration of the cysteine can be detected visually according to the color change of the system.

The invention relates to a keratin nano-flower material loaded with silver phosphate and preparation and application thereof. The preparation method of the keratin nano-flower material comprises the preparation of keratin nano-flowers and loading of the silver phosphate. The material is used as a catalyst for the detection of uric acid. Compared with the prior art, the material has the advantages of simple preparation process, good operability, high recovery rate, environmental protection and high efficiency, and is easy to be separated from a solution, and keratine-nano flower @ Ag3PO4 has excellent peroxidase-like catalytic activity, and can be used as a novel simulated enzyme to replace hydrogen peroxide oxidase and be widely used in biological detection, clinical diagnosis and immune analysis. The keratine-nano flower @ Ag3PO4 is used for colorimetric detection of the uric acid in serum, the detection process is simple and fast, economical and environment-friendly, and the colorimetric detection of the uric acid does not need to be marked, the detection range is wide, and the selectivity is good; even when the concentration of interfering substances is 3.3 times of the concentration of the uric acid, the keratin nano-flower material can still detect uric acid efficiently.

The invention, which relates to the technical fields of nano material, catalysis and chemical sensing, provides a novel copper-doped-carbon-nano-dot-based method for enzyme-ratio-free fluorescence detection of the organophosphorus pesticide. With anhydrous copper chloride, tris(hydroxymethyl) aminomethane and L-dopa as raw materials, fluorescent copper-doped carbon nano-dots with high stability and peroxidase activity are synthesized by a one-step hydrothermal method. O-phenylenediamine oxidized by hydrogen peroxide is catalyzed based on peroxidase-like activity of the copper-doped carbon nano-dots and fluorescent oxidized molecule 2,3-diaminophenazine is genreated, so that two fluorescent emission peaks of the copper-doped carbon nano-dot and 2,3-diaminophenazine are generated at the lengthes of 375 nm and 555 nm; and the ratio of the fluorescence intensities at the two wavelengths is linearly related to the concentration of the organophosphorus pesticide, thereby realizing enzyme-ratio-free fluorescence detection of the organophosphorus pesticide. The sensor is constructed simply and easily; no biological enzyme is needed; the influences of the probe concentration and environmental factor are low; and the linear range is wide and the sensitivity is high.

The invention provides a cleavage type universal-partition ultrafast-amplification visual sensor for zinc ions. The visual sensor comprises (1) a cleavage system, (2) a sPCR amplification system, (3)a HCR system, and (4) a detection system containing an ABTS developing solution. According to the invention, primers, a template and probes are ingeniously designed, so ultrafast amplification of thetemplate can be realized in the presence of zinc ions, and an amplification product is allowed to form a G-quadruplex in a proper environment by triggering a HCR reaction; and color development is performed by utilizing the peroxidase-like activity of the G-quadruplex. Thus, the problem of difficulty in realizing visual detection of traditional PCR products is overcome, and rapid and visual detection of zinc ions is realized. Moreover, the sensor provided in the invention has the characteristics of high specificity and high sensitivity to zinc ions, and is more objective and accurate in detection results.

A catalyst use of a hydrogen ion containing micromolecule as a peroxidase-like enzyme is disclosed. That the hydrogen ion containing micromolecule has peroxidase-like activity is disclosed for the first time. The hydrogen ion containing micromolecule is definite in molecular structure, easily available, high in stability, cheap and prone to commercial development. Based on the use, a novel methodfor detection hydrogen peroxide is successfully established and is successfully applied in test paper for hydrogen peroxide detection. The micromolecule having peroxidase-like activity can also be applied in food, environment, sanitation, and other fields and has a wide and good application prospect.

The invention provides a visual detection method of magnesium and zinc cutting type functional nucleic acids for universal partition and ultra-fast amplification. The visual detection method providedby the invention can perform ultra-fast amplification on templates the presence of magnesium and zinc ions by ingeniously designing primers, the templates and probes, enables amplification products toform a G-quadruplex in a suitable environment, further uses peroxidase-like activity of the G-quadruplex for color development, solves a problem that traditional PCR products are hard to be detectedvisually, and realizes fast and visual detection of magnesium and zinc ions. Moreover, the visual detection method provided by the invention has the characteristics of high specificity and high sensitivity to magnesium and zinc ions, and more objective and accurate detection results.

The invention provides a universal-partition ultrafast-amplification cleavage type functional-nucleic acid visual detection method for copper and calcium. According to the invention, primers, a template and probes are ingeniously designed, so ultrafast amplification of the template can be realized in the presence of copper and calcium ions, and amplification products are allowed to form G-quadruplexes in a proper environment; and color development is performed by utilizing the peroxidase-like activity of the G-quadruplexes. Thus, the problem of difficulty in realizing visual detection of traditional PCR products is overcome, and rapid and visual detection of copper and calcium ions is realized. Moreover, the method provided in the invention has the characteristics of high specificity and high sensitivity to copper and cadmium ions, and is more objective and accurate in detection results.

The invention belongs to the technical field of composite materials, and discloses a preparation method and application of double-layer metal organic skeleton immobilized HRP. The preparation method comprises the steps: firstly, preparing Fe-MIL-88A, and enabling Fe-MIL-88A to react with precursor copper acetate of HKUST-1 and 1,3,5-trimellitic acid (H3BTC) in sequence, so as to prepare Fe-MIL-88A@Cu<2+>@H3BTC; and finally, immobilizing horse radish peroxidase (HRP) in a skeleton by adopting a coprecipitation method to obtain a composite material Fe-MIL-88A@HRP@HKUST-1. The prepared compositematerial has strong stability, has high catalytic activity under acidic and high temperature conditions, and still retains 90% or above of the activity after 10 cycles. Besides, Fe-MIL-88A and HKUST-1in the composite material both have peroxidase-like activity; when the composite material is used for immobilizing HRP, the stability of HRP can be improved, cyclic utilization of HRP is achieved, the synergistic catalysis effect can be achieved through coupling of HRP and the skeleton, and the catalytic activity of the composite material is further improved. The composite material is used for degrading harmful dyes in wastewater, is small in dosage, short in time and simple and convenient to operate, and has a good application prospect.

The invention discloses an As(V) dual-mode detection method based on a peroxidase-like characteristic of a CoOOH nanosheet, and belongs to the field of absorption spectrums and electrochemical sensing. The method comprises the following steps: firstly, the CoOOH nanosheet is synthesized, and when H2O2 exists, CoOOH can catalyze a developing substrate AABTS to be converted into a green oxidation product ABTSox; when As(V) exists, As(V) is combined onto the surface of the CoOOH under the electrostatic action and the action of an As-O bond, so that the catalysis activity of the CoOOH to the ABTSis inhibited, leading to reduction of the green oxidation product ABTSox and color lighting, and detection of the As(V) can be realized according to an absorption spectrum; in addition, the CoOOH is modified onto the surface of a glassy carbon electrode, and when the As(V) is adsorbed onto the surface of the CoOOH modified electrode, high-sensitivity electrochemical analysis of the As(V) can be realized according to an attenuation degree of reducing current. The dual-mode detection method disclosed by the invention has the advantages of simplicity, sensitivity, visibility, dual-mode detectionand the like.

The invention relates to the fields of nano materials, catalysis and analysis chemistry, and specifically relates to preparation and application of a pesticide rapid test card. According to the method, cobalt oxide nanospheres with peroxidase-like activity, acetylcholinesterase, choline oxidase and a color developing agent are used to modify filter paper so as to obtain a rapid test card for rapiddetection. The rapid test card detects acetylcholine and organic phosphorus pesticide easily and rapidly. The novel colorimetric pesticide detection method is highly practical, thereby having a broadapplication prospect in fields like food detection, analysis chemistry, environment engineering and agriculture.

A probe that changes solution color in the presence of only one of the two DNA sequences, which differ by a single nucleotide, is reported. The probe consists of two oligodeoxyribonucleotides, which form a hydrogen peroxidase-like DNA enzyme when hybridized to the abutting fragments of the complementary analyte. The active peroxidase catalyses oxidation of colorless substrates to the colored products. The probe allows visual detection of a mutation in Alzheimer's disease-related DNA.

The invention relates to the field of analysis, in particular to a method for detecting mercury ions by chitosan-gold nanoparticles. The method includes: mixing chitosan-gold nanoparticles, Hg<2+> solution, 3,3',5,5'-tetramethyl benzidine solution and H2O2 solution, culturing for 5-30min at 10-90 DEG C, and using an ultraviolet spectrophotometer for detecting mercury ions, wherein the chitosan-gold nanoparticles is prepared by steps: mixing HAuCl4 solution, chitosan solution and deionized water, heating to boil, performing reflux stirring to the end of reaction, and cooling to the room temperature. The chitosan-gold nanoparticles have peroxidase activity which can be enhanced by mercury ions, so that the chitosan-gold nanoparticles can be used for mercury ion detection. The method is simple, convenient, quick and low in time consumption in a whole operating process and beneficial to quick detection of the mercury ions.

The invention discloses a novel method for preparing an rGO-ZnFe2O4 nanometer enzyme with a peroxidase-like property. On the basis, the catalysis performance of the nanometer enzyme is used for building a novel visual method for detecting the glucose content in urine; under the best condition, a glucose linear range measured by the method is 0.5 to 50 mu M; a linear regression equation is A=0.010c(mu M)+0.19(R=0.9913); a detection limit is 0.16 mu M. By using the method, whether a person suffers from diabetes or not can be judged by people with naked eyes.

A complex comprising a carrier containing a plasma-derived protein or serum-derived protein solidified by denaturation coagulation, and a protein having a peroxidase-like activity and carried by the carrier, which has such high effectiveness that it can strongly stimulate an antigen-presenting cell, and can be used as a highly safe immunostimulant with reduced toxicity to living bodies.

The invention belongs to the field of composite material, and particularly relates to PEG-modified magnetic/quaternized chitosan magnetic nanoparticles with peroxidase activity, and a preparation method and application thereof. The PEG-modified magnetic/quaternized chitosan magnetic nanoparticles with peroxidase activity comprise highly magnetic Fe3O4 as a magnetic core, a crosslinked polyamide/chitosan polyelectrolyte complex in the middle layer, and a quaternized chitosan and hydrophilic polyethylene glycol chain as the outer shell. The intermediate polymer layer adequately protects the high magnetic property and peroxidase activity of the Fe3O4, and the quaternized chitosan and hydrophilic polyethylene glycol chain as the shell provides long-term water stability and dispersibility for the nanoparticles. Nanoparticles have a fast and efficient "encirclement and suppression" effect on bacterial pathogens. The strategy uses a bacterial capture nanocomposite containing the magnetic nanoparticles with peroxidase-like activity under a trace amount of H2O2.

The invention discloses a glucose detection method based on peroxidase-like catalysis characteristics of graphene quantum dots, and belongs to the technical field of optical sensing. The graphene quantum dots have the peroxidase-like catalytic activity, can catalyze hydrogen peroxide reduction in the presence of hydrogen peroxide, also can oxidize colorless reduced-form tetramethyl benzidine into oxidized-form tetramethyl benzidine to show blue and produce an ultraviolet absorption peak at 652nm. In the presence of oxygen, glucose oxidase catalyzes oxidation of glucose to produce the hydrogen peroxide, the hydrogen peroxide reduction is catalyzed by use of the peroxidase-like catalysis characteristics of the graphene quantum dots, and the reduced-form tetramethyl benzidine in the solution is oxidized to show color. The glucose concentration is greater, the blue color of the solution is deeper, and the ultraviolet absorption strength is stronger, so that fast, sensitive and visual glucose detection can be realized.

The invention discloses tin-antimony oxide nanoparticles with a peroxidase-like characteristic and a preparation method and an application thereof. The tin-antimony oxide nanoparticles are prepared mainly by the steps: mixing and heating tin, antimony trioxide and concentrated sulfuric acid, then successively adding hydrogen peroxide and ultrapure water, carrying out hydrothermal reaction, finally, centrifuging and separating a precipitate, washing, drying and grinding to obtain the tin-antimony oxide nanoparticles containing tin, antimony and oxygen elements. The preparation method has the advantages of sufficient sources of used raw materials, low production cost, simple material preparation process, simple operation and strong repeatability; the obtained tin-antimony oxide nanoparticleshave uniform crystal grain size, can be uniformly dispersed in an aqueous phase in the presence of triethylamine, can catalyze the oxidation of a chromogenic substrate 3,3',5,5'-tetramethylbenzidineby hydrogen peroxide, and have great potential applications in the fields of food testing, pollutant treatment and the like.

This disclosure provides a method for polymerizing polyphenols to provide polyphenol polymers using peroxidase and similar catalysis. In various aspects, it provides a method for polymerizing a polyphenol (e.g., polydopamine or a derivative or conjugate thereof) on a surface comprising polymerizing the polyphenol, a method for detecting an analyte comprising polymerizing a polyphenol, and an assay kit comprising a polyphenol (e.g., dopamine or a dopamine derivative). In one embodiment, a method for polymerizing a polyphenol includes contacting the polyphenol and an oxidant with an enzyme having peroxidase-like activity, under conditions sufficient to polymerize the polyphenol. In another embodiment, a method for depositing a polyphenol polymer (e.g., a polydopamine) includes providing, at a target site, an enzyme having peroxidase-like activity immobilized at the surface; and polymerizing, at the target site, a polyphenol in the presence of an oxidant and the enzyme to provide the polyphenol polymer, deposited on the surface.

The invention provides a biomass amorphous carbon having oxidase-like and peroxidase-like activity, and a preparation method and application thereof. With enteromorpha as a raw material, simple high-temperature calcination is performed to obtain biomass amorphous carbon having oxidase-like and peroxidase-like activity simultaneously. According to the biomass amorphous carbon, acid phosphatase is detected qualitatively and quantitatively based on the oxidase-like property. The biomass material with the low cost is available; the preparation method is simple; and the green and environment-friendly effects are good. Therefore, the biomass amorphous carbon has the broad application prospects in analytical chemistry, catalysis, biomedicine and environmental engineering fields.