15 results about "Peroxidase like" patented technology

The invention relates to the technical field of nano-enzymes, and discloses a copper-based nano-enzyme with laccase and peroxidase double-enzyme activity and preparation and application thereof, and the copper-based nano-enzyme is a nano-material prepared by forming MOF through solvothermal reaction of copper ions and diamino terephthalic acid and then performing electrostatic adsorption on the MOF and polyoxometallate. The copper-based nano enzyme has high-efficiency laccase-like activity and peroxidase-like activity at the same time through the combination and synthesis process of the copper ion cluster and the organic ligand. The invention further discloses a preparation method of the copper-based nano-enzyme, peroxidase activity detection and application of the copper-based nano-enzyme to degradation and determination of dichlorophenol. The copper-based nano-enzyme provided by the invention has high catalytic activity and stability, is low in cost and easy to prepare, and can be applied to biosensing detection of hydrogen peroxide and environmental remediation degradation of organic pollutants such as phenols and dyes.

The application discloses a preparation method of carbon dot nanometer enzyme, which comprises the following steps: dissolving oxalic acid, polyacetyl imine and ferric nitrate in ultrapure water, heating and mixing, cooling to room temperature, centrifuging, filtering, dialyzing and taking supernatant to obtain the carbon dot nanometer enzyme; and a double-mode detection method for colorimetric and fluorescent detection of glyphosate in fruits and vegetables is constructed by using the prepared oxalic acid carbon dot nanometer enzyme, acetylcholinesterase, acetylcholine, 3,3',5,5'-tetramethyl benzidine and H2O2. The oxalic acid carbon dot nanometer enzyme is prepared by doping ferric nitrate and tetraethylenepentamine with oxalic acid as a precursor, has excellent peroxidase-like activity, and a colorimetric and fluorescent double-mode detection method for glyphosate is constructed by using the oxalic acid carbon dot nanometer enzyme, the double-mode detection results can be verified with each other, and the accuracy and reliability of the detection results are significantly improved.

The invention discloses a pentagonal porphyrinyl covalent organic framework material as well as a preparation method and application thereof. According to the material, 5, 10, 15, 20-tetra (4-aminophenyl) nickel porphyrin and N, N '-bis (4-formylphenyl)-N, N'-diphenyl benzidine are used as construction units, and a two-dimensional covalent organic framework structure with pentagonal pore topological characteristics is formed through a Schiff base condensation reaction. Ni-N4 coordination structures which are uniformly distributed in the material construct catalytic sites similar to a natural enzyme active center in a non-uniform pore microenvironment, and the material shows excellent and stable peroxidase-like catalytic activity. The preparation method of the material has the advantages of clear process steps and mild reaction conditions, and is suitable for stable and repeated preparation. A catechol inhibition type colorimetric sensing system constructed on the basis of the material has good analysis performance in the aspects of detection linear range, detection limit and the like and does not need to depend on large-scale instruments and equipment, and a detection result can be observed through naked eyes.

The invention discloses preparation and application of a nano enzyme. The preparation method of the nano enzyme comprises the following steps: dissolving copper salt and hydroxy anthraquinone carboxylic acid in water to obtain a first solution; adjusting the pH of the first solution to be alkaline, and heating for reaction to obtain a product solution; and centrifuging the product solution, taking the precipitate, washing and drying to obtain the Cu nano-enzyme. The copper salt comprises at least one of copper sulfate, copper chloride, copper nitrate and copper hydroxide; the hydroxy anthraquinone carboxylic acid is rhein. According to the invention, Cu is used as a transition metal source and reacts with specific hydroxy anthraquinone carboxylic acid to prepare the Cu nano-enzyme (Cu-Rhein) with excellent peroxidase-like activity. The Cu nano enzyme can be applied to promotion of hydrogen peroxide oxidation TMB color development and detection of penicillamine, the accuracy of penicillamine detection is high, and the Cu nano enzyme has good application prospects.

The invention discloses a cerium-based polyoxometallate / metal organic framework-based composite nano-enzyme as well as a preparation method and detection application of the cerium-based polyoxometallate / metal organic framework-based composite nano-enzyme. The composite material is prepared by reacting ferric chloride, cerium-based polyacid and 2-aminoterephthalic acid, and has excellent peroxidase-like activity, stability and substrate affinity. Based on the material, the invention further develops a recyclable paper-based detection device, and the paper-based detection device is matched with a smart phone color recognition system to construct a rapid colorimetric detection platform for the antioxidant. The platform can realize high-sensitivity and high-selectivity detection of various antioxidants such as ascorbic acid, glutathione, gallic acid and the like, and is successfully applied to total antioxidant capacity analysis of actual samples such as fruits and the like. Compared with a traditional method, the method has the advantages of being rapid in detection, easy and convenient to operate, low in cost, environmentally friendly and the like, and is suitable for the field of food safety on-site rapid monitoring.

The invention discloses a molecular imprinting colorimetric sensor for detecting hepatitis B virus as well as a preparation method and application of the molecular imprinting colorimetric sensor. The sensor takes an aminated zeolite imidazate framework material (ZIF-8-NH2) with peroxidase-like activity as a catalytic core, after surface double-bond functionalization, a molecularly imprinted polymer (MIPs) layer is constructed on the surface through a free radical polymerization reaction by taking the aminated zeolite imidazate framework material as a carrier and taking HBV as a template molecule. According to the sensor, after HBV is specifically recognized and captured through MIPs, the catalytic efficiency of ZIF-8 on hydrogen peroxide is changed, then the generation amount of hydroxyl radicals is regulated and controlled, and finally visual and quantitative detection of HBV is achieved through the color fading reaction of the hydroxyl radicals and chromogenic dye crystal violet. The sensor prepared by the invention has the advantages of high sensitivity, strong specificity, low cost, simplicity and convenience in operation, rapidness in detection and the like, and can be used for rapid screening and on-site instant detection of hepatitis B viruses.

The invention discloses screening of a Cronobacter sakazakii aptamer and development of a functional nucleic acid double-recognition element biosensor. The screening comprises the following steps: (1) determining a sequence of the Cronobacter sakazakii aptamer; (2) optimizing reaction conditions of the cronobacter sakazakii biosensor; and (3) carrying out visual detection on the cronobacter sakazakii. The detection principle of the sensor is as follows: an aptamer is coupled to a carboxyl magnetic bead to serve as a capture element, enrichment of target bacterial cells is realized through magnetic separation, and a large amount of dissolved contents provide a target with a higher order of magnitude for ribozyme cutting by splitting the target bacterial cells. Short-chain nucleic acid generated after ribozyme cutting is used as a primer of RCA amplification reaction, generation of a product with a large number of G-quadruplex sequences can be triggered, and finally, TMB is catalyzed to generate chromogenic reaction by utilizing peroxidase-like activity of a G-quadruplex / hemin compound, so that visual output of signals can be realized, and ultra-sensitive detection of cronobacter sakazakii is completed.

The application discloses a bacteriophage-nanocatalyst hybrid nanorobot and method for drug-resistant bacteria control. The nanorobot comprises a lytic bacteriophage capable of specifically recognizing target drug-resistant bacteria, and a metal nanocatalyst with peroxidase-like catalytic activity; the metal nanocatalyst is covalently connected to the bacteriophage coat protein and fixed on the surface of the bacteriophage after being surface-modified by a polyethylene glycol connecting molecule containing an active group. The preparation method comprises the steps of bacteriophage separation and purification, nanocatalyst synthesis, introduction of an active group on the surface of the nanocatalyst, coupling with the bacteriophage and product purification. The nanorobot has the abilities of targeted adsorption and catalytic production of active oxygen, and can be used for killing drug-resistant bacteria, destroying biofilms and degrading antibiotic resistance genes in the environment, and is suitable for scenes such as sewage treatment and biofilm pollution control.

The invention discloses a PtPdCuFe alloy nano enzyme material, and belongs to the technical field of nano materials and analytical chemistry. According to the technical key points, four metal atoms of platinum, palladium, copper and iron are uniformly distributed on the nanoscale to form an alloy structure, and the unique quaternary composition and atomic-scale mixing generate a synergistic effect, so that the catalyst has excellent oxidase-like and peroxidase-like activities at the same time. The invention also discloses a colorimetric sensor containing the material, and a method for detecting in a buffer system through catalytic oxidation and coupling with nitrite by using the oxidase-like activity of the material. According to the method, the peroxidase-like activity of the material is utilized, and TMB oxidation is inhibited through thiophanate-methyl in the presence of hydrogen peroxide for detection. The invention further discloses a method for synthesizing the quaternary alloy material through a one-step hydrothermal method. The method is mainly used for high-sensitivity and high-selectivity rapid colorimetric analysis of nitrite pollutants and thiophanate-methyl pesticide residues in food and environmental samples.

The invention discloses a bimetallic nano-enzyme as well as a preparation method and application thereof, and relates to the technical field of biological detection. The bimetallic nano-enzyme is a DNA-Ag / Pt bimetallic nano-enzyme material prepared on the basis of a specific DNA template sequence or containing the specific DNA template sequence, and can be combined with a double-emission ratio type silver nano-cluster to be used for double-signal detection of vibrio parahaemolyticus. The bimetallic nano-enzyme has peroxidase-like activity, the enzyme activity efficiency is adjustable, and the bimetallic nano-enzyme can be used for double-signal detection of vibrio parahaemolyticus. Compared with the prior art, the color development absorbance of the bimetallic nano-enzyme provided by the invention is improved by about 15%, the lowest detection limit of a colorimetric detection method is 10 CFU / mL, and the lowest detection limit of a fluorescence detection method is 1 CFU / mL; the method has no cross reaction on staphylococcus aureus, escherichia coli, pseudomonas aeruginosa, salmonella, vibrio vulnificus and the like, and is suitable for on-site rapid screening, visual interpretation and semi-quantitative detection of scenes such as water samples and marine products.

The application provides a colloidal molecule system based on DNA circuit coding assembly and a preparation method thereof. The application develops DNA circuit coding CMs with high precision and time dependence by using ssDNA with alternating polyA domains and functional domains and dynamic DNA circuit catalytic hairpin self-assembly (CHA) to code and assemble AuNPs, and the DNA circuit coding CMs can be used for adjusting peroxidase-like activity of AuNPs. The application can obtain DNA circuit coding CMs with dimeric, linear or reticular structures by changing the number and length of the polyA domains and the functional domains. The application can accurately adjust the peroxidase-like catalytic activity of the DNA circuit coding CMs system by blocking the catalytic active sites on the surface of the AuNPs or simply changing the concentration of the external DNA stimulant. The application provides a new idea for regulating the catalytic function of nanomaterials.

The application discloses a preparation method of nanometer material with biomimetic enzyme activity and application of the nanometer material in glyphosate detection, wherein the nanometer material with biomimetic enzyme activity is heptanoic acid and Prussian blue modified Fe3O4 nanoparticles, Fe3O4@C7 / PB with core-shell structure is obtained, the core is ferroferric oxide, and the shell layer is formed by compounding heptanoic acid and Prussian blue. The application has the beneficial effect that: the Fe3O4 nanoparticles modified by heptanoic acid and Prussian blue synthesize a nanometer enzyme (Fe3O4@C7 / PB) with peroxidase-like activity, the peroxidase-like activity is higher than that of Fe3O4@C7, and Fe3O4@C7 / PB can be kept stable through single-layer PB to prevent agglomeration. We establish an anti-interference intelligent mobile phone assisted nanometer sensing platform based on peroxidase-like activity of Fe3O4 nanoparticles (Fe3O4@C7 / PB) modified by heptanoic acid and Prussian blue (PB), which is used for determining glyphosate content in tobacco, and the detection limit is 0.1 ug / mL ‑1 .

The application relates to the fields of material preparation and chemical analysis, in particular to a kind of preparation of MnO2 / AuOs composite material and method for detecting Pb 2+ Using MnO2 / AuOs. MnO2 / AuOs preparation includes MnO2 nanosheet, Au seed, AuOs, MnO2 / AuOs preparation. NaOH is added to manganese acetate solution, stirred, centrifuged, and the precipitate is dispersed in 2-mercaptoethanol aqueous solution to obtain MnO2 nanosheet. Gold seed is obtained by the reaction of tetrachloroauric acid and NaBH4, K2OsCl6 and ascorbic acid are added to the gold seed solution to obtain AuOs. AuOs is added to the MnO2 nanosheet solution, stirred to obtain MnO2 / AuOs composite material. Different concentrations of Pb 2+ are added to the MnO2 / AuOs composite material, acetic acid-sodium acetate buffer solution, 3,3',5,5'-tetramethylbenzidine solution and H2O2 solution are added, and Pb 2+ is detected by ultraviolet spectrophotometer. The MnO2 / AuOs composite material has peroxidase-like activity, Pb 2+ can form an alloy with gold in the material in the presence of 2-mercaptoethanol, thereby enhancing the peroxidase-like activity, so the composite nanomaterial can be used for detecting Pb 2+ .

The application provides a Prussian blue composite material and a preparation method and application thereof, and belongs to the technical field of biological medicines.The Prussian blue composite material provided by the application comprises hollow mesoporous Prussian blue nanoparticles, liposomes coated on the hollow mesoporous Prussian blue nanoparticles and borane ammine complexes loaded on the hollow mesoporous Prussian blue nanoparticles.The hollow mesoporous Prussian blue nanoparticles of the application can simulate various enzyme-like activities to remove ROS, such as superoxide dismutase, catalase or peroxidase-like activities to relieve inflammatory damage caused by oxidative stress;the borane ammine complexes are loaded on the hollow mesoporous Prussian blue nanoparticles, the borane ammine complexes can continuously supply hydrogen, reach the inflammation site of dry eye syndrome, and thus relieve the symptoms of dry eye syndrome in combination with the hollow mesoporous Prussian blue nanoparticles;the liposomes can specifically deliver the Prussian blue composite material to specific cells or tissues, so as to improve the targeting and curative effect of the drug and relieve the symptoms of dry eye syndrome.

The invention discloses a nano enzyme and an application thereof in glutathione recognition and / or detection, the nano enzyme Fe3O4 at W18O49 comprises a Fe3O4 microsphere and a W18O49 nano cluster compounded on the Fe3O4 microsphere, and the nano enzyme Fe3O4 at W18O49 has peroxidase-like catalytic activity, and can catalyze a chromogenic reaction between 3, 3 ', 5, 5'-tetramethyl benzidine and H2O2. The W18O49 coated Fe3O4 microsphere material is used as the nano-enzyme for the first time, the catalytic performance of the material is remarkably improved, compared with natural enzyme or other mimetic peroxidase, the material has better applicability, and the core of Fe3O4 endows the material with magnetic recoverability, so that the nano-enzyme can be used for many times, and the consumption cost of the material is reduced. According to the glutathione detection method and the glutathione detection system provided by the invention, rapid detection of glutathione can be realized without the help of other large-scale detection equipment, and the method and the system have relatively good convenience.