Ratio-type nano silicon quantum dot fluorescence probe and preparation method and application thereof

A nano-silicon quantum dot and silicon quantum dot technology, applied in the fields of nanomaterials and molecular biology, can solve the problems of poor imaging effect and high background, and achieve the effects of stable signal, high sensitivity and high specificity

Active Publication Date: 2016-07-27
FUDAN UNIV
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  • Claims
  • Application Information

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Problems solved by technology

However, if this probe is directly applied to cell imaging to detect the content of hydroxyl radicals in the body, the strong

Method used

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  • Ratio-type nano silicon quantum dot fluorescence probe and preparation method and application thereof
  • Ratio-type nano silicon quantum dot fluorescence probe and preparation method and application thereof
  • Ratio-type nano silicon quantum dot fluorescence probe and preparation method and application thereof

Examples

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[0035] Example 1

[0036] (1) Preparation of silicon quantum dots

[0037] Before preparation, first soak a 25mL round bottom flask with aqua regia (concentrated hydrochloric acid: concentrated nitric acid = 3:1), soak overnight, wash it, dry it, and cool to room temperature. Adjust the speed of the magnetic stirrer, add 1mL triaminopropyl-triethoxysilane (APTES), 5mL water, 1mL 0.1M sodium ascorbate (NaAA) solution at room temperature, stir for 4h, the solution turns from clear and transparent to pink. Then silicon quantum dots are formed.

[0038] (2) Synthesis of silicon quantum dot-chlorin e6 composite

[0039] The synthesized silicon quantum dots (1.9mL) and 50μMCe6 (0.1mL) were mixed at room temperature and protected from light, and left to stand for two days and nights. Chlorin e6 was adsorbed to the surface of silicon quantum dots by electrostatic action, and obtained Silicon quantum dot-chlorin e6 composite material, denoted as Si-ce6.

[0040] After obtaining Si-ce6, use a ...

Example Embodiment

[0047] Example 2

[0048] (1) Preparation of silicon quantum dots

[0049] Before preparation, first soak a 25mL round bottom flask with aqua regia (concentrated hydrochloric acid: concentrated nitric acid = 3:1), soak overnight, wash it, dry it, and cool to room temperature. Adjust the rotation speed of the magnetic stirrer, add 2mL triaminopropyl-triethoxysilane (APTES), 10mL water, 2mL 0.1M sodium ascorbate (NaAA) solution at room temperature, stir for 4.5h, the solution turns from clear to pink , Then silicon quantum dots are generated.

[0050] (2) Synthesis of silicon quantum dot-chlorin e6 composite

[0051] The synthesized silicon quantum dots (2.5mL) and 75μMCe6 (0.1mL) were mixed at room temperature and protected from light, and allowed to stand for two days and nights. Chlorin e6 was adsorbed to the surface of the silicon quantum dots by electrostatic action. Silicon quantum dot-chlorin e6 composite material, denoted as Si-ce6.

[0052] After obtaining Si-ce6, use a 3K ult...

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Abstract

The invention belongs to the technical field of nano material science and molecular biology, and particularly relates to a ratio-type nano silicon quantum dot fluorescence probe and a preparation method and application thereof. The preparation method comprises the steps that a one-pot method is adopted, triaminopropyl-triethoxysilane is reduced with sodium ascorbate at room temperature, and water-soluble silicon quantum dots are obtained; the water-soluble silicon quantum dots and chlorin e6 (Ce6) are mixed and subjected to standing at the room temperature under the lucifugal condition, and a silicon quantum dot and chlorin e6 composite material is obtained. The composite material emits fluorescence with the wavelength of 430 nm-580 nm and the wavelength of 640 nm-680 nm under the fluorescence excitation of 410 nm, and active oxygen radicals can specifically quench fluorescence of silicon quanta and basically have no influence on fluorescence of Ce6. Therefore, the composite material can serve as a ratio-type nano silicon quantum dot fluorescence probe to be used for detecting the content of hydroxyl radicals (-OH), rapidness and high efficiency are achieved, the specificity is high, the sensitivity is high, the probe is used for in-vivo cell imaging, the biocompatibility is good, and the visual degree is high.

Description

technical field [0001] The invention belongs to the technical fields of nanomaterials and molecular biology, and specifically relates to a nanometer quantum dot fluorescent probe and a preparation method thereof, as well as an application in high-sensitivity and high-selectivity detection of hydroxyl radicals and cell imaging. Background technique [0002] Silicon quantum dots have good luminescent properties, and have attracted extensive attention from researchers in materials science, chemical biology, and life sciences in recent years. In the past five years, many researchers have done a lot of research on the synthesis method of silicon quantum dots. Silicon quantum dots have the characteristics of good biocompatibility, high fluorescence quantum yield and no photobleaching. They are ideal fluorescent probes in biological imaging applications and have great application value in the field of life sciences. [0003] Reactive oxygen species (ReactiveOxygenSpecies, ROS) sub...

Claims

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Application Information

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IPC IPC(8): C09K11/06C07D487/22G01N21/64
CPCC07D487/22C09K11/06C09K2211/1029C09K2211/1074G01N21/6428
Inventor 陈惠赵倩倩叶代新孔继烈
Owner FUDAN UNIV
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