Heparin detection method for copper nano-cluster based on denatured bovine serum protein as template
A technology of bovine serum albumin and copper nanoclusters, which is applied in the field of aqueous phase synthesis of copper nanoclusters, can solve the problems of safety and biocompatibility, biological detection limits, etc., and achieve wide detection linear range and detection limit The effect of low and uniform particle size
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[0038] Example 1
[0039] 0.1M CuCl 2 Solution preparation: Weigh 1.7050g CuCl 2 Dissolve in an appropriate amount of high-purity water, transfer to a 100mL volumetric flask to make a constant volume, label it for later use;
[0040] Denaturation of bovine serum albumin: weigh 0.1200g bovine serum albumin and dissolve it in 30mL high-purity water, fully dissolve under stirring conditions, weigh out 0.0040g NaBH 4 Add to the above-mentioned bovine serum albumin solution, fully react for 1h, heat the above-mentioned mixed solution at 70°C for 30min, and cool to room temperature;
[0041] Synthesis of copper nanoclusters: Add prepared CuCl to the above denatured bovine serum albumin solution under stirring conditions 2 250μL of solution, after full reaction, add 0.7mL of hydrazine hydrate to the above mixed solution, and react for 2h under stirring conditions until the solution turns pale yellow;
Example Embodiment
[0042] Example 2
[0043] 0.1M CuCl 2 Solution preparation: Weigh 1.7050g CuCl 2 Dissolve in an appropriate amount of high-purity water, transfer to a 100mL volumetric flask to make a constant volume, label it for later use;
[0044] Denaturation of bovine serum albumin: weigh 0.1200g bovine serum albumin and dissolve it in 30mL high-purity water, fully dissolve under stirring conditions, weigh out 0.0040g NaBH 4 Add to the above-mentioned bovine serum albumin solution, fully react for 1h, heat the above-mentioned mixed solution at 70°C for 30min, and cool to room temperature;
[0045] Synthesis of copper nanoclusters: Add prepared CuCl to the above denatured bovine serum albumin solution under stirring conditions 2 250μL of the solution, after full reaction, add 0.7mL of hydrazine hydrate to the above mixed solution, and react for 6h under stirring conditions until the solution turns pale yellow;
Example Embodiment
[0046] Example 3
[0047] 0.1M CuCl 2 Solution preparation: Weigh 1.7050g CuCl 2 Dissolve in an appropriate amount of high-purity water, transfer to a 100mL volumetric flask to make a constant volume, label it for later use;
[0048] Denaturation of bovine serum albumin: weigh 0.0800g bovine serum albumin and dissolve it in 30mL of high-purity water, dissolve it fully under stirring conditions, weigh out 0.0040g NaBH 4 Add to the above-mentioned bovine serum albumin solution, fully react for 1h, heat the above-mentioned mixed solution at 70°C for 30min, and cool to room temperature;
[0049] Synthesis of copper nanoclusters: Add prepared CuCl to the above denatured bovine serum albumin solution under stirring conditions 2 250μL of the solution, after full reaction, add 0.7mL of hydrazine hydrate to the above mixed solution, and react for 6h under stirring conditions until the solution turns pale yellow;
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