144results about How to "Wide detection linear range" patented technology

The invention discloses a full-scale range C-reactive protein latex-enhanced immunoturbidimetry detection kit. Through turbidity change, quantitative determination is realized. The kit comprises a reagent R1 and a reagent R2. The reagent R1 is a phosphate buffer solution. The reagent R2 comprises two conjugates with different particle sizes, and the conjugates comprise a conjugate of carboxylic polystyrene latex and a rat anti-human CRP antibody and a conjugate of carboxylic polystyrene latex and a mouse anti-human CRP antibody. The kit can be combined with a full-automatic specific protein analyzer so that detection sensitivity and accuracy are improved and detection time is shortened. The full-scale range C-reactive protein latex-enhanced immunoturbidimetry detection kit can detect high concentration CRP content and low concentration CRP content and has the advantages of high sensitivity, strong stability, wide linear range, good repeatability and cheap price.

The invention discloses a lipoprotein-related phospholipase A2 content detection kit and a preparation method thereof, belonging to the field of detection of lipoprotein-related phospholipase A2 content. The kit consists of a reagent I and a reagent II which are independent of each other, wherein the reagent I comprises the following components: a biological buffer agent, a coagulant, a preservative, a chelating agent and water; the reagent II comprises the following components: latex particles coated with a lipoprotein-related phospholipase A2 antibody, a biological buffer agent, a surfactant, a preservative, a suspending agent and water. The latex particles coated with the lipoprotein-related phospholipase A2 antibody are prepared by adopting latex particles with the particle size of 120-180nm, and the stability, sensitivity and wide detection linear range of the kit can be effectively guaranteed. Meanwhile, the latex particles coated with the lipoprotein-related phospholipase A2 antibody are prepared by adopting a specific method, so that the bottle-opening stability and detection specificity of the kit are relatively good, and the product is suitable for popularization and application of clinical detection.

The invention discloses a multi-channel three-dimensional microfluidic paper chip and a preparation method thereof. The multi-channel three-dimensional microfluidic paper chip uses a piece of upper layer filter paper A and a piece of lower layer filter paper B printed with different microfluidic channels to form a three-dimensional microfluidic channel system through corresponding overlapping. Themulti-channel microfluidic paper chip provided by the invention realizes the simultaneous fast and sensitive determination of different substances, and can be used in the fields of biochemical analysis, immunoassay, sensors, instant detection device study and the like.

The invention relates to a method of trace detection of a copper ion through a CoNiO2 nanomaterial modified glassy carbon electrode. The method comprises the steps as follows: mixing a saline solutionof Co and Ni ions with sodium hydroxide for a reaction to generate a sediment, performing hydrothermal treatment on the sediment at a certain temperature, performing microwave treatment on a Co-Ni bimetallic material after the hydrothermal treatment to form a CoNiO2 nanomaterial, and finally dispersedly modifying the CoNiO2 nanomaterial on a glassy carbon electrode for heavy metal copper ion detection. The method can achieve high selectivity detection of copper ions and has the advantages of high detection sensitivity, good stability, high interference immunity, wide linear detection range and the like. According to the method, the traditional calcining method is replaced with a microwave method, the problems of uneven distribution of particle size and pattern, long preparation cycle, serious energy consumption and the like of the traditional calcining method are solved, and a new approach that has an excellent effect and is convenient to popularize and apply is provided.

The invention relates to a kit for rapidly diagnosing lipoprotein-associated phospholipase A2 and a use method of the kit. The kit comprises a standard substance, a quality control substance, a liposome complex, a magnetic separation reagent, an assay buffer solution and a cleaning solution, wherein the inner part of the liposome complex is coated with N-hydroxysuccinimide ester of tris (bipyridine) ruthenium; an antibody for resisting the lipoprotein-associated phospholipase A2 is connected to the surface of the liposome complex by interaction of streptavidin and biotin; the magnetic separation reagent takes magnetic particulates containing amino terminals as a carrier and is connected with the antibody for resisting the lipoprotein-associated phospholipase A2 through the interaction of the streptavidin and the biotin. Compared with an enzyme linked immunosorbent assay kit produced in a foreign Diadexus company, the kit prepared by the invention has the advantages that sensitivity and precision for analyzing the lipoprotein-associated phospholipase A2 are greatly improved; in addition, the kit disclosed by the invention is simple and convenient to operate, quick and easier to popularize.

The invention relates to production of a low-density carbon nanotube array composite electrode and the application of the same and in a glucose sensor. A carbon nanotube array is directionally grown on a substrate of the low-density carbon nanotube array composite electrode; metal nanoparticles adhere to the top and the tube wall of a carbon nanotube of the carbon nanotube array; the density of the carbon nanotube array is 10<8> to 10<10> every square centimeter. The response of the low-density carbon nanotube array composite electrode to a glucose solution is good under the non-enzymatic condition, the sensitivity can reach 1000 to 1500 microamperes millimeter<minus 1> centimeter<minus 2>, the linear detection range is 5 microns to 7 millimeters, and the influence of common interfering substances can be eliminated. The low-density carbon nanotube array composite electrode can be directly produced on a silicon substrate and accordingly the combination with a semiconductor or MEMS (Micro-Electro-Mechanical System) process is good.

The present invention discloses a method for electrochemical detection of antibiotics in milk based on vertical and ordered micelle enrichment. The method is characterized by comprising: diluting milk with an electrolyte solution so as to be adopted as a detected solution, adopting vertical and ordered CTAB micelle modified electrode as a working electrode, using an electrochemical voltammetry method, enriching antibiotics in the milk solution, and then detecting. According to the present invention, the vertical and ordered micelle modified electrode is adopted as the working electrode, the hydrophobic and electrically neutral antibiotics in the aqueous solution are extracted/enriched into the hydrophobic nanometer chambers of the surfactant micelles through the hydrophobic effect, and the reduction peak current of the antibiotics is adopted as the detection signal so as to achieve the quantitative detection in the complex milk background; and the constructed sensor integrated the pre-enrichment and the electrochemical detection, has characteristics of high sensitivity, wide linear detection range and low detection limit, and has great application potential in the food safety monitoring field.

The invention discloses a method for simultaneously detecting ascorbic acid, dopamine, uric acid, tryptophan and nitrite.The method is implemented by a three-electrode system sensor with a working electrode, a reference electrode and a counter electrode.The working electrode is made of graphene/tantalum, graphene/boron-doped diamond films and graphene/titanium; the reference electrode can be a saturated calomel electrode or an Ag/AgCl electrode or a mercury/mercurous sulfate electrode; the counter electrode is a platinum sheet electrode.The method includes acquiring working curves by the aid of mixed solution of five to-be-detected materials with different concentrations and differential pulse voltammetry; diluting human serum; adjusting the concentrations until the concentrations are within the ranges of the working curves; comparing peak currents obtained by the aid of the differential pulse voltammetry to the working curves to obtain the concentrations of the five to-be-detected materials in the serum.The method has the advantages that characteristics of the concentrations of the five to-be-detected materials can be quickly simultaneously detected, and the method is high in sensitivity and low in detection limit.

The invention relates to a method for detecting fungaltoxin by using a photonic crystal micro-sphere liquid-phase chip chemiluminiscence method. According to the method, photonic crystal micro-spheres are used as probe carriers of multiple fungaltoxins, the fungaltoxins are detected by carrying out competitive immunodetection on the surfaces of the micro-spheres, the multiple fungaltoxins are competed with antigens fixed on the surfaces of the micro-spheres so as to be combined with respective anti-bodies of the fungaltoxins, second antibodies marked by using horse radish peroxidases are specifically combined with respective fungaltoxin antibody, reflection peaks of the photonic crystal micro-spheres are measured by using a fiber optic spectrometer so as to decode the micro-spheres, and high throughput tests are carried out on chemiluminiscence signals by using a multi-functional microplate reader. The linear detection ranges of the method to aflatoxin, fumonisins and ochratoxin are respectively 0.0001-1ng/mL, 0.0001-1ng/mL and within 0.001-1ng/mL, and only 2mu L is needed for a sample.

The invention discloses a kit for quantitatively detecting GFAP concentration in human serum by polystyrene microsphere, comprising a 96-hole filter plate, a standard product, a sample diluent, a polystyrene microsphere coupled with an anti-GFAP antibody on the surface, a microsphere storage solution, an enzyme compound, a substrate A, a substrate B, a 20*cleaning solution, an adhesive sticker strip and an operating manual. The kit provided by the invention changes the solid phase carrier made of traditional microporous plate into the polystyrene microsphere, generates the antigen antibody reaction in the liquid phase to keep a good bioactivity and a correct spatial configuration, and eliminates the effect on the antigen antibody reaction by the surface tension and the steric hindrance ofthe traditional reaction mode, and effectively shortens the reaction time and the rinsing times, besides, the invention has the characteristics of simple operation, high sensitivity, good specificityand low cost and has important clinical significance for the diagnosis of the acute cerebral hemorrhage early disease degree.

The invention discloses a kit for detecting TORCH IgM antibodies. The kit comprises a solid carrier which is coated with an antibody combined with the TORCH IgM antibodies, a TORCH antigen which is marked with lanthanide, a TORCH IgM antibody calibrator, a sample diluent, an experiment buffering solution, a concentration cleaning solution and an enhancement solution. The kit can simultaneously detect multiple pathogene IgM antibodies in TORCH pathogene under the same detection condition and is high in sensitivity, high in specificity, good in precision, high in accuracy, little in blood consumption amount, rapid, convenient and favorable for early diagnosis on the infection of the TORCH pathogene. The method has the characteristic that the detection linear range is wide, the diluting times or diluting ratio of a high-value sample can be reduced, and the accuracy of a detection result can be improved.

The invention, which belongs to the technical field of medical in-vitro diagnostics, relates to a human urine immunoglobulin G detection kit based on latex-enhanced immunoturbidimetry, thereby solvinga problem of providing a human urine immunoglobulin G content detection kit having advantages of wide detection linear range, high detection accuracy, and high detection stability. The kit comprisesan R1 reagent, an R2 reagent and a calibrator. The R1 reagent includes a buffer solution, NaCl and a preservative. The R2 reagent includes a buffer solution, an antibody-coupling latex microsphere, aprotective agent and a preservative; and the antibody is the goat anti-human immunoglobulin polyclonal antibody or a rabbit anti-human immunoglobulin polyclonal antibody. The calibrator is formed by aplurality of solution; each solution includes human immunoglobulin G, a buffer solution and a preservative; and the concentrations of the human immunoglobulin G in different solutions are different.The kit has advantages of wide detection linear range, high detection accuracy, and high detection stability.

The application discloses a kit for detecting human heparin binding protein and a preparation method thereof. The kit for latex enhanced immunoturbidimetry detection comprises a reagent 1, a reagent 2and a calibration product. The reagent 2 contains HBP-monoclonal-antibody-labeled large-sphere latex microspheres and HBP-monoclonal-antibody-labeled small-sphere latex microspheres, wherein a particle size difference between the large-sphere latex microsphere and the small-sphere latex microsphere is at least 200 nm and a surface charge difference between the small-sphere latex microspheres andthe large-sphere latex microspheres is at least 100 [mu] eq/g. According to the application, the large-sphere and small-sphere latex microspheres with the particle size difference value of at least 200nm and the surface charge difference value of at least 100 [mu]eq/g are used for HBP monoclonal antibody labeling to realize latex enhanced immunoturbidimetry detection, so that the detection sensitivity can be improved and the wider detection linear range can be obtained. A high-sensitivity and full-scale detection kit is provided for HBP clinical detection.

The invention provides construction of a multi-layer structure surface enhanced Raman base and accurate regulation and control for performance of the construction, relating to the technical field of Raman detection. The construction comprises four steps of selecting a base, constructing a dual-layer nano-structure, pre-treating the dual-layer nano-structure and constructing a Raman base; a traditional single-layer structure can be transformed into a dual-layer structure through a simple replacement reaction, the enhancing factor of Raman is increased by 1.5 times; by electroplating or spinninggraphene, the Raman enhancing performance is further improved due to the graphene layer, which is 1.48 times that of the dual-layer structure, and the long-term stability of the base can be further improved. The multi-layer structure surface enhanced Raman base can be used for improving the limiting concentration of a traditional Raman base for detecting probe molecules and further improving enhancing factors of the traditional Raman base, and is low in cost and simple to operate; and the preparation process realizes rapid reaction without large reaction equipment, and accords with environment-friendly chemical concept.

The invention discloses a kit for quantitatively detecting HCV-cAg concentration in human serum by virtue of polystyrene microspheres, comprising a 96-hole filter plate, a standard product, sample diluent, microsphere suspension, enzyme conjugate, substrate A, substrate B, 20* cleaning solution, adhesive sticker sealing strip and operating instruction. In the kit disclosed by the invention, a solid phase carrier is changed into polystyrene microspheres from the traditional micropore board, and reaction between antigen and antibody takes place in liquid phase, thus the antigen and antibody canbeneficially maintain bioactivity and correct space conformation, and influence of surface tension and space obstacle of the traditional reaction mode to antigen antibody reaction can be eliminated, the two advantages are all beneficial to reaction of the antigen and the antibody, and reaction time and washing frequency can be effectively reduced; meanwhile, the kit disclosed by the invention hasthe characteristics of simple operation, high sensitivity, good specificity and low cost and has important clinical significance on diagnosis of 'window period' of a hepatitis C virus infestor.

The invention relates to a method for detecting aflatoxin B1 with high sensitivity in a single-bead photonic crystal microsphere suspension array chemiluminescence way. The method comprises the following steps of: adopting photonic crystal microspheres as a probe carrier of the aflatoxin B1 to perform competitive immunodetection to the aflatoxin B1 on the surfaces of the microspheres; performing competitive binding to a monoclonal toxin antibody through an antigen fixed to the surfaces of the microspheres, of the aflatoxin B1 in a sample; performing specific binding to the aflatoxin B1 antibody through a second antibody marked by horse radish peroxidase; and then detecting a chemiluminiscence light signal through a multifunctional microplate reader. According to the method, the aflatoxin detection limit to the aflatoxin B1 is 0.0001ng/mL, the linear detection range is 0.0001 to 1ng/ml, and only 2mu L sample is needed.

The invention provides a kit for improving GPC3 detection sensitivity and a preparation method thereof, and belongs to the technical field of immunodetection; the kit comprises a reagent M and a reagent R; the reagent M is prepared by diluting a magnetic bead particle coupled mouse anti-human GPC3 monoclonal antibody, and the diameter of the magnetic bead particle is 0.8-3[mu]m; the diluent of themagnetic bead particle coupled mouse anti-human GPC3 monoclonal antibody comprises the following components: Procline 300, a buffer solution, a surfactant and protein; and the reagent R is prepared by diluting a rabbit anti-human GPC3 polyclonal antibody marked by alkaline phosphatase. The kit has the advantages of higher GPC3 detection sensitivity and wider linear range. The invention also provides a preparation method of the kit for improving the GPC3 detection sensitivity.

The invention provides a method and a kit for quantitative combined detection of PA (Prealbumin) and CRP (C-reactive Protein), as well as a preparation method and application of the kit. The kit comprises a test paper strip I and a test paper strip II, which are used for detecting PA and CRP, wherein the test paper strip I is composed of a bottom plate I, a sample pad I, a combination pad I, a nitrocellulose membrane I and water absorption paper I; and the test paper strip II is composed of a bottom plate II, a sample pad II, a combination pad II, a nitrocellulose membrane II and water absorption paper II. The detection method comprises the following steps: (1) adding a PA standard object into the sample pad I and adding a CRP standard object into the sample pad II; after reacting, drawing a standard curve according to a specific value of the fluorescence intensity of a detection line and the fluorescence intensity of a quality control line; and (2) adding a solution of an object to be detected into the sample pad I and the sample pad II, and reading PA content and CRP content in the solution of the object to be detected according to the standard curve. The kit and the detection method, provided by the invention, are simple and convenient to operate, high in clinical acceptance degree and good in detection effect.

The invention discloses an immunity latex turbidimetry kit for detecting NGAL (neutrophil gelatinase associated lipocalin) based on single-grain-size latex particles. The kit comprises a reagent R1 and a reagent R2; the reagent R1 is prepared from buffer solutions, surfactants, coagulants, chelating agents, preservatives, reductants, electrolytes and water; the reagent R2 is prepared from the buffer solutions, stabilizers, electrolytes and latex particles coated with antihuman NGAL polyclonal antibodies; the reductants are beta-mercaptoethanol; the antihuman NGAL polyclonal antibodies are biotin-labeled antihuman NGAL polyclonal antibodies; the latex particles are streptavidin-labeled single-grain-size latex particles. The kit has the advantages that NGAL in blood and urine samples can bedetected at the same time, so that the detection sensitivity is improved, the linear range is widened, and influences of interference factors can be avoided; NGAL homodimers and heterodimers are detected, and accordingly, misjudgment of doctors due to low NGAL detection results is avoided.