144results about How to "Wide detection linear range" patented technology

The invention provides a simulative-target metabonomics analytic method based on combination of liquid chromatography and mass spectrum. The method includes following steps: respectively manufacturing to-be-analyzed samples into merged samples according to groups; automatically collecting secondary mass spectrums of metabolites in each merged samples through data of UHPLS / Q-TOF MS with dependence on a collecting mode; extracting a retaining time and information of parent ions and daughter ions of the metabolites by qualitative analytic software; screening out characteristic ion pair information of the metabolites according to daughter ion response intensity; summing the characteristic ion pair information of each merged samples; and scanning the obtained characteristic ion pair of the metabolites by UHPLC / QQQ MS through a dynamic multi-reaction monitoring mode in an actual sample for obtaining a corresponding spectrogram; and performing integration through quantitative analytic software to obtain the metabolites in the to-be-analyzed samples and content information thereof.

The invention discloses a full-scale range C-reactive protein latex-enhanced immunoturbidimetry detection kit. Through turbidity change, quantitative determination is realized. The kit comprises a reagent R1 and a reagent R2. The reagent R1 is a phosphate buffer solution. The reagent R2 comprises two conjugates with different particle sizes, and the conjugates comprise a conjugate of carboxylic polystyrene latex and a rat anti-human CRP antibody and a conjugate of carboxylic polystyrene latex and a mouse anti-human CRP antibody. The kit can be combined with a full-automatic specific protein analyzer so that detection sensitivity and accuracy are improved and detection time is shortened. The full-scale range C-reactive protein latex-enhanced immunoturbidimetry detection kit can detect high concentration CRP content and low concentration CRP content and has the advantages of high sensitivity, strong stability, wide linear range, good repeatability and cheap price.

The invention aims at providing an electrochemical sensor for sensitively detecting heavy metal cadmium ions and a preparation method of the electrochemical sensor. The structure of the sensor is formed by an electrochemical workstation, an electrolytic cell and electrodes; the electrodes include a platinum wire counter electrode, a silver / silver chloride reference electrode and a working electrode, wherein the working electrode refers to a glassy carbon electrode adopting nitrogen-sulfur codoping and graphite porous carbon / Nafion / bismuth film modification. The electrochemical sensor can realize sensitive detection of trace heavy metal cadmium ions, the detection linear range is 4-80 micrograms / L, and the detection limit reaches 0.1 microgram / L. By combining with good properties of a bismuth film, a Nafion film and a nitrogen-sulfur codoped porous carbon material, the electrochemical sensor has the performances of high sensitivity, good selectivity, wide linear range, good reproducibility, good stability and the like and can be applied to detecting the heavy metal cadmium ions in an actual water sample.

The invention relates to a dinucleotide-labelled ratio electrochemical immunosensor. The dinucleotide-labelled ratio electrochemical immunosensor is characterized in that DNA3 of sulfydryl and ferrocene are respectively labelled at the two assembled ends of the surface of a gold electrode and are hybridized with methylene-blue-labelled complementary DNA1-antibody1 so as to form a ratio electrochemical immunosensor interface. When target protein exists, a DNA2-antibody2, the target protein and the DNA1-antibody1 form sandwiched immune complex to generate a vicinal effect, and DNA2 is hybridized with DNA1, so that the DNA1-antibody1 is separated from a sensing interface, and freed DNA3 forms a hairpin structure on the surface. In the process, methylene blue and the ferrocene with electrochemical activity are separated from and close to the surface of the electrode respectively, and the oxidized electric currents of the methylene blue and the ferrocene are reduced and increased respectively. By detecting the ratio of the two currents, the concentration of the target protein in the solution is detected. The immunosensor is high in sensitivity, wide in the detection range, realizes fast and one-step detection of protein, and has clinical application value.

The invention discloses a lipoprotein-related phospholipase A2 content detection kit and a preparation method thereof, belonging to the field of detection of lipoprotein-related phospholipase A2 content. The kit consists of a reagent I and a reagent II which are independent of each other, wherein the reagent I comprises the following components: a biological buffer agent, a coagulant, a preservative, a chelating agent and water; the reagent II comprises the following components: latex particles coated with a lipoprotein-related phospholipase A2 antibody, a biological buffer agent, a surfactant, a preservative, a suspending agent and water. The latex particles coated with the lipoprotein-related phospholipase A2 antibody are prepared by adopting latex particles with the particle size of 120-180nm, and the stability, sensitivity and wide detection linear range of the kit can be effectively guaranteed. Meanwhile, the latex particles coated with the lipoprotein-related phospholipase A2 antibody are prepared by adopting a specific method, so that the bottle-opening stability and detection specificity of the kit are relatively good, and the product is suitable for popularization and application of clinical detection.

The invention discloses a multi-channel three-dimensional microfluidic paper chip and a preparation method thereof. The multi-channel three-dimensional microfluidic paper chip uses a piece of upper layer filter paper A and a piece of lower layer filter paper B printed with different microfluidic channels to form a three-dimensional microfluidic channel system through corresponding overlapping. Themulti-channel microfluidic paper chip provided by the invention realizes the simultaneous fast and sensitive determination of different substances, and can be used in the fields of biochemical analysis, immunoassay, sensors, instant detection device study and the like.

The invention relates to a method of trace detection of a copper ion through a CoNiO2 nanomaterial modified glassy carbon electrode. The method comprises the steps as follows: mixing a saline solutionof Co and Ni ions with sodium hydroxide for a reaction to generate a sediment, performing hydrothermal treatment on the sediment at a certain temperature, performing microwave treatment on a Co-Ni bimetallic material after the hydrothermal treatment to form a CoNiO2 nanomaterial, and finally dispersedly modifying the CoNiO2 nanomaterial on a glassy carbon electrode for heavy metal copper ion detection. The method can achieve high selectivity detection of copper ions and has the advantages of high detection sensitivity, good stability, high interference immunity, wide linear detection range and the like. According to the method, the traditional calcining method is replaced with a microwave method, the problems of uneven distribution of particle size and pattern, long preparation cycle, serious energy consumption and the like of the traditional calcining method are solved, and a new approach that has an excellent effect and is convenient to popularize and apply is provided.

The invention relates to the preparation and application of nano-materials, in particular to a Prussian blue nano-particle preparation method through reverse micelle. The invention aims to overcome the disadvantages of poor stability of Prussian blue and easy reunion of nano-particles. The Prussian blue nano-particles prepared through reverse micelle are small in particle size and are dispersed evenly. The nano-particles are decorated on an electrode to detect H2O2 so as to realize the electrochemical analysis and application of the nano-particles; and the nano-particles are characterized by high sensitivity, good stability, low detection limit and wide H2O2 analysis and detection linear range. Enzyme can be fixed on the surface of the electrode modified by the nano-particles to prepare an enzyme biosensor so as to realize detection and analysis of the corresponding enzyme substrates and reduce the cost of enzyme biosensors.

The invention relates to a kit for rapidly diagnosing lipoprotein-associated phospholipase A2 and a use method of the kit. The kit comprises a standard substance, a quality control substance, a liposome complex, a magnetic separation reagent, an assay buffer solution and a cleaning solution, wherein the inner part of the liposome complex is coated with N-hydroxysuccinimide ester of tris (bipyridine) ruthenium; an antibody for resisting the lipoprotein-associated phospholipase A2 is connected to the surface of the liposome complex by interaction of streptavidin and biotin; the magnetic separation reagent takes magnetic particulates containing amino terminals as a carrier and is connected with the antibody for resisting the lipoprotein-associated phospholipase A2 through the interaction of the streptavidin and the biotin. Compared with an enzyme linked immunosorbent assay kit produced in a foreign Diadexus company, the kit prepared by the invention has the advantages that sensitivity and precision for analyzing the lipoprotein-associated phospholipase A2 are greatly improved; in addition, the kit disclosed by the invention is simple and convenient to operate, quick and easier to popularize.

The invention provides preparation and application of an immunochromatography method and test paper for quantitative detection of prealbumin. The test paper consists of a bottom plate, a sample pad, a conjugate pad, a nitrocellulose membrane and a piece of water absorbing paper, wherein the sample pad, the conjugate pad, the nitrocellulose membrane and the water absorbing paper are sequentially adhered to the bottom plate in a lap joint manner and are respectively and partially overlapped; a detection line and a quality control line are arranged on the nitrocellulose membrane; the quality control line is wrapped with a donkey-anti-rat antibody. A method comprises the following steps: (1) adding a solution which is of known concentration and comprises a prealbumin standard substance onto the sample pad of the test paper, and drawing a standard curve; (2) adding a solution of a substance to be tested, performing reaction, and reading content according to standard curves. The test paper and the detection method provided by the invention are high in sensitivity, that is, up to pg / mL, wide in detection linearity, convenient to operate, and short in time, that is, results can be obtained within only 10-20 minutes.

The invention relates to production of a low-density carbon nanotube array composite electrode and the application of the same and in a glucose sensor. A carbon nanotube array is directionally grown on a substrate of the low-density carbon nanotube array composite electrode; metal nanoparticles adhere to the top and the tube wall of a carbon nanotube of the carbon nanotube array; the density of the carbon nanotube array is 10<8> to 10<10> every square centimeter. The response of the low-density carbon nanotube array composite electrode to a glucose solution is good under the non-enzymatic condition, the sensitivity can reach 1000 to 1500 microamperes millimeter<minus 1> centimeter<minus 2>, the linear detection range is 5 microns to 7 millimeters, and the influence of common interfering substances can be eliminated. The low-density carbon nanotube array composite electrode can be directly produced on a silicon substrate and accordingly the combination with a semiconductor or MEMS (Micro-Electro-Mechanical System) process is good.

The invention aims at providing an electrochemical sensor for sensitively and selectively detecting dopamine (DA) and uric acid (UA) simultaneously and a preparation method. The sensor structurally comprises an electrochemical working station, an electrolytic cell and electrodes, wherein the electrodes include a platinum-wire counter electrode, a silver / silver chloride reference electrode and a working electrode; the working electrode means a glass-carbon electrode modified by microwave-peeled porous carbon. The electrochemical sensor provided by the invention has excellent properties such ashigh sensitivity, good selectivity, wide linear range and low detection limit in detecting the DA and the UA since the microwave-peeled porous carbon for modifying the electrode has excellent properties such as large specific surface area, porous structure, excellent electrocatalytic activity property and good conductivity. The electrochemical sensor can be used for qualitatively and quantitatively detecting the contents of the DA and the UA in actual biological samples.

The present invention discloses a method for electrochemical detection of antibiotics in milk based on vertical and ordered micelle enrichment. The method is characterized by comprising: diluting milk with an electrolyte solution so as to be adopted as a detected solution, adopting vertical and ordered CTAB micelle modified electrode as a working electrode, using an electrochemical voltammetry method, enriching antibiotics in the milk solution, and then detecting. According to the present invention, the vertical and ordered micelle modified electrode is adopted as the working electrode, the hydrophobic and electrically neutral antibiotics in the aqueous solution are extracted / enriched into the hydrophobic nanometer chambers of the surfactant micelles through the hydrophobic effect, and the reduction peak current of the antibiotics is adopted as the detection signal so as to achieve the quantitative detection in the complex milk background; and the constructed sensor integrated the pre-enrichment and the electrochemical detection, has characteristics of high sensitivity, wide linear detection range and low detection limit, and has great application potential in the food safety monitoring field.

The invention discloses an ECL (electrochemiluminescence) determining method for ATP (adenosine triphosphate). According to the ECL determining method for ATP, gold nanoclusters with high quantum yield are taken as an ECL probe, a manganese dioxide nanomaterial is taken as a quenching agent, when ATP exists, an aptamer can be specifically bound with ATP and DNAzyme cannot be formed, glutathione and manganese dioxide on an electrode surface are subjected to a redox reaction, ECL signals of the gold nanocluster probe are restored gradually with increase of ATP concentration, and content of ATP is determined. Logarithm of ATP concentration in the concentration range of 3.6*10<-12>-3.6*10<-3> mol / L has a linear relation with the change value of ECL intensity, and limit of detection is 1.4*10<-12> mol / L. The method is simple to operate, low in cost, high in sensitivity and good in reproducibility and can be used for determining ATP in an actual sample, thereby having better clinical application prospect.

The invention discloses a method for simultaneously detecting ascorbic acid, dopamine, uric acid, tryptophan and nitrite.The method is implemented by a three-electrode system sensor with a working electrode, a reference electrode and a counter electrode.The working electrode is made of graphene / tantalum, graphene / boron-doped diamond films and graphene / titanium; the reference electrode can be a saturated calomel electrode or an Ag / AgCl electrode or a mercury / mercurous sulfate electrode; the counter electrode is a platinum sheet electrode.The method includes acquiring working curves by the aid of mixed solution of five to-be-detected materials with different concentrations and differential pulse voltammetry; diluting human serum; adjusting the concentrations until the concentrations are within the ranges of the working curves; comparing peak currents obtained by the aid of the differential pulse voltammetry to the working curves to obtain the concentrations of the five to-be-detected materials in the serum.The method has the advantages that characteristics of the concentrations of the five to-be-detected materials can be quickly simultaneously detected, and the method is high in sensitivity and low in detection limit.

The invention discloses a preparation method of a nitrogen and chlorine double-doped fluorescent carbon quantum dot. The preparation method comprises the steps of with amino sugar hydrochloride or amino acid hydrochloride as raw materials, simultaneously supplying a carbon source, a nitrogen source and a chlorine source, dissolving the raw material into ultrapure water, stirring so as to obtain asettled solution, carrying out hydrothermal reaction, cooling a synthesized product, filtering, and carrying out centrifugation and dialysis, so as to obtain the water-soluble nitrogen and chlorine double-doped fluorescent carbon quantum dot. By utilizing a one-step reaction, the required raw material is few, byproducts are few, and the method has the characteristics of high efficiency, economy and the like. The carbon quantum dot prepared by virtue of the carbon quantum dot has stable fluorescent property and low toxin, can be applied to the detection of Fe<3+> and simultaneously has wide application prospect in the aspects of fluorescent ink, sewage treatment and the like.

The invention discloses a preparation method of a Co4S3 / nitrogen doped graphene composite material and application thereof, and belongs to the field of inorganic material synthesis and analysis. The composite material is prepared as follows: firstly using ammonia water to regulating alkaline environment, then using hydrazine hydrate and graphene oxide for reaction and conversion into nitrogen doped graphene, and then adding cobalt acetate and thiourea to prepare the Co4S3 / nitrogen doped graphene composite material by a solvent thermal process. The method has simple process and low cost, the main reaction is performed in an aqueous phase, reaction conditions are mild, an electrochemical sensor constructed by use of the Co4S3 / nitrogen doped graphene composite material can show a strong catalytic effect on electrochemical reduction of hydrogen peroxide because of synergistic effect of the nitrogen doped graphene with a high surface area, high conductivity and good biocompatibility and a Co4S3 nano material with good electronic transfer characteristics, and the electrochemical sensor has the advantages of wide detection linear range, low detection limit and high sensitivity and selectivity, and is successfully used to detect the hydrogen peroxide in real samples.

The invention relates to a method for detecting fungaltoxin by using a photonic crystal micro-sphere liquid-phase chip chemiluminiscence method. According to the method, photonic crystal micro-spheres are used as probe carriers of multiple fungaltoxins, the fungaltoxins are detected by carrying out competitive immunodetection on the surfaces of the micro-spheres, the multiple fungaltoxins are competed with antigens fixed on the surfaces of the micro-spheres so as to be combined with respective anti-bodies of the fungaltoxins, second antibodies marked by using horse radish peroxidases are specifically combined with respective fungaltoxin antibody, reflection peaks of the photonic crystal micro-spheres are measured by using a fiber optic spectrometer so as to decode the micro-spheres, and high throughput tests are carried out on chemiluminiscence signals by using a multi-functional microplate reader. The linear detection ranges of the method to aflatoxin, fumonisins and ochratoxin are respectively 0.0001-1ng / mL, 0.0001-1ng / mL and within 0.001-1ng / mL, and only 2mu L is needed for a sample.

The invention discloses a kit for quantitatively detecting GFAP concentration in human serum by polystyrene microsphere, comprising a 96-hole filter plate, a standard product, a sample diluent, a polystyrene microsphere coupled with an anti-GFAP antibody on the surface, a microsphere storage solution, an enzyme compound, a substrate A, a substrate B, a 20*cleaning solution, an adhesive sticker strip and an operating manual. The kit provided by the invention changes the solid phase carrier made of traditional microporous plate into the polystyrene microsphere, generates the antigen antibody reaction in the liquid phase to keep a good bioactivity and a correct spatial configuration, and eliminates the effect on the antigen antibody reaction by the surface tension and the steric hindrance ofthe traditional reaction mode, and effectively shortens the reaction time and the rinsing times, besides, the invention has the characteristics of simple operation, high sensitivity, good specificityand low cost and has important clinical significance for the diagnosis of the acute cerebral hemorrhage early disease degree.

The invention discloses a kit for detecting TORCH IgM antibodies. The kit comprises a solid carrier which is coated with an antibody combined with the TORCH IgM antibodies, a TORCH antigen which is marked with lanthanide, a TORCH IgM antibody calibrator, a sample diluent, an experiment buffering solution, a concentration cleaning solution and an enhancement solution. The kit can simultaneously detect multiple pathogene IgM antibodies in TORCH pathogene under the same detection condition and is high in sensitivity, high in specificity, good in precision, high in accuracy, little in blood consumption amount, rapid, convenient and favorable for early diagnosis on the infection of the TORCH pathogene. The method has the characteristic that the detection linear range is wide, the diluting times or diluting ratio of a high-value sample can be reduced, and the accuracy of a detection result can be improved.

The invention, which belongs to the technical field of medical in-vitro diagnostics, relates to a human urine immunoglobulin G detection kit based on latex-enhanced immunoturbidimetry, thereby solvinga problem of providing a human urine immunoglobulin G content detection kit having advantages of wide detection linear range, high detection accuracy, and high detection stability. The kit comprisesan R1 reagent, an R2 reagent and a calibrator. The R1 reagent includes a buffer solution, NaCl and a preservative. The R2 reagent includes a buffer solution, an antibody-coupling latex microsphere, aprotective agent and a preservative; and the antibody is the goat anti-human immunoglobulin polyclonal antibody or a rabbit anti-human immunoglobulin polyclonal antibody. The calibrator is formed by aplurality of solution; each solution includes human immunoglobulin G, a buffer solution and a preservative; and the concentrations of the human immunoglobulin G in different solutions are different.The kit has advantages of wide detection linear range, high detection accuracy, and high detection stability.

The application discloses a kit for detecting human heparin binding protein and a preparation method thereof. The kit for latex enhanced immunoturbidimetry detection comprises a reagent 1, a reagent 2and a calibration product. The reagent 2 contains HBP-monoclonal-antibody-labeled large-sphere latex microspheres and HBP-monoclonal-antibody-labeled small-sphere latex microspheres, wherein a particle size difference between the large-sphere latex microsphere and the small-sphere latex microsphere is at least 200 nm and a surface charge difference between the small-sphere latex microspheres andthe large-sphere latex microspheres is at least 100 [mu] eq / g. According to the application, the large-sphere and small-sphere latex microspheres with the particle size difference value of at least 200nm and the surface charge difference value of at least 100 [mu]eq / g are used for HBP monoclonal antibody labeling to realize latex enhanced immunoturbidimetry detection, so that the detection sensitivity can be improved and the wider detection linear range can be obtained. A high-sensitivity and full-scale detection kit is provided for HBP clinical detection.

The invention provides construction of a multi-layer structure surface enhanced Raman base and accurate regulation and control for performance of the construction, relating to the technical field of Raman detection. The construction comprises four steps of selecting a base, constructing a dual-layer nano-structure, pre-treating the dual-layer nano-structure and constructing a Raman base; a traditional single-layer structure can be transformed into a dual-layer structure through a simple replacement reaction, the enhancing factor of Raman is increased by 1.5 times; by electroplating or spinninggraphene, the Raman enhancing performance is further improved due to the graphene layer, which is 1.48 times that of the dual-layer structure, and the long-term stability of the base can be further improved. The multi-layer structure surface enhanced Raman base can be used for improving the limiting concentration of a traditional Raman base for detecting probe molecules and further improving enhancing factors of the traditional Raman base, and is low in cost and simple to operate; and the preparation process realizes rapid reaction without large reaction equipment, and accords with environment-friendly chemical concept.

The invention discloses a kit for quantitatively detecting HCV-cAg concentration in human serum by virtue of polystyrene microspheres, comprising a 96-hole filter plate, a standard product, sample diluent, microsphere suspension, enzyme conjugate, substrate A, substrate B, 20* cleaning solution, adhesive sticker sealing strip and operating instruction. In the kit disclosed by the invention, a solid phase carrier is changed into polystyrene microspheres from the traditional micropore board, and reaction between antigen and antibody takes place in liquid phase, thus the antigen and antibody canbeneficially maintain bioactivity and correct space conformation, and influence of surface tension and space obstacle of the traditional reaction mode to antigen antibody reaction can be eliminated, the two advantages are all beneficial to reaction of the antigen and the antibody, and reaction time and washing frequency can be effectively reduced; meanwhile, the kit disclosed by the invention hasthe characteristics of simple operation, high sensitivity, good specificity and low cost and has important clinical significance on diagnosis of 'window period' of a hepatitis C virus infestor.

The invention relates to a method for detecting aflatoxin B1 with high sensitivity in a single-bead photonic crystal microsphere suspension array chemiluminescence way. The method comprises the following steps of: adopting photonic crystal microspheres as a probe carrier of the aflatoxin B1 to perform competitive immunodetection to the aflatoxin B1 on the surfaces of the microspheres; performing competitive binding to a monoclonal toxin antibody through an antigen fixed to the surfaces of the microspheres, of the aflatoxin B1 in a sample; performing specific binding to the aflatoxin B1 antibody through a second antibody marked by horse radish peroxidase; and then detecting a chemiluminiscence light signal through a multifunctional microplate reader. According to the method, the aflatoxin detection limit to the aflatoxin B1 is 0.0001ng / mL, the linear detection range is 0.0001 to 1ng / ml, and only 2mu L sample is needed.

The invention provides a kit for improving GPC3 detection sensitivity and a preparation method thereof, and belongs to the technical field of immunodetection; the kit comprises a reagent M and a reagent R; the reagent M is prepared by diluting a magnetic bead particle coupled mouse anti-human GPC3 monoclonal antibody, and the diameter of the magnetic bead particle is 0.8-3[mu]m; the diluent of themagnetic bead particle coupled mouse anti-human GPC3 monoclonal antibody comprises the following components: Procline 300, a buffer solution, a surfactant and protein; and the reagent R is prepared by diluting a rabbit anti-human GPC3 polyclonal antibody marked by alkaline phosphatase. The kit has the advantages of higher GPC3 detection sensitivity and wider linear range. The invention also provides a preparation method of the kit for improving the GPC3 detection sensitivity.

The invention discloses a zinc ion fluorescent probe, a preparation method and a method for detecting zinc ion content. The chemical formula of the fluorescent probe is as shown in the specification, wherein R1 is -Cl, -Br, -I, -NO2 or NH2; R2 is -Cl, -Br, -I, -NO2 or NH2. The preparation method is characterized in that ammonium acetate and TBAB are used as the catalysts, 1, 3-amino alcohol, a aniline derivative and a benzaldehyde derivative are used as the raw materials, and a series of Zn<2+> fluorescent probes in a water phase under microwave conditions. The zinc ion fluorescent probe is good in structural stability. The preparation method is simple and easy to operate, high in product yield and free of pollutant generation. The detection method has the advantages that the detection method is simple, the Zn<2+> content can be detected in a quantified manner only by detecting fluorescence intensity, and the method is good in detection accuracy; a used solvent is an ethanol solution which is nontoxic and environmentally friendly; the method is wide in detection linear range, detection limit of the method is 1*10<-6>mol / L, and the method is applicable to the detection of the trace Zn<2+> content of environments and food and the in-vivo and in-vitro trace Zn<2+> content.

The invention relates to an air coupling ultrasound-based ballastless track plate detection device. The output end of a signal transmitting and receiving element is connected with the input end of a data processing unit through an A / D conversion circuit and a built-in amplifier; the data processing unit is connected with a storage unit; the signal transmitting and receiving element emits transmitting signal to a signal transmitting probe group; the signal transmitting and receiving element receives the echo signals of a signal receiving probe group through an external amplifier; and the signal transmitting probe group and the signal receiving probe group are horizontally arranged above a steel track. With the method disclosed by the invention adopted, the comprehensive scanning of the interlayer delamination and water seepage of a track plate and a mortar layer, the block missing of the track plate, and the internal deep crack of the track plate can be performed. The air coupling ultrasound-based ballastless track plate detection device has the advantages of many defect scanning types, wide detection linear range and high detection efficiency. With the air coupling ultrasound-based ballastless track plate detection device adopted, the visual display of a detection result is realized; and a bonding condition between the track plate and the mortar layer is judged according to the phase change condition of echo signals.

The invention provides a method and a kit for quantitative combined detection of PA (Prealbumin) and CRP (C-reactive Protein), as well as a preparation method and application of the kit. The kit comprises a test paper strip I and a test paper strip II, which are used for detecting PA and CRP, wherein the test paper strip I is composed of a bottom plate I, a sample pad I, a combination pad I, a nitrocellulose membrane I and water absorption paper I; and the test paper strip II is composed of a bottom plate II, a sample pad II, a combination pad II, a nitrocellulose membrane II and water absorption paper II. The detection method comprises the following steps: (1) adding a PA standard object into the sample pad I and adding a CRP standard object into the sample pad II; after reacting, drawing a standard curve according to a specific value of the fluorescence intensity of a detection line and the fluorescence intensity of a quality control line; and (2) adding a solution of an object to be detected into the sample pad I and the sample pad II, and reading PA content and CRP content in the solution of the object to be detected according to the standard curve. The kit and the detection method, provided by the invention, are simple and convenient to operate, high in clinical acceptance degree and good in detection effect.

The invention discloses an immunity latex turbidimetry kit for detecting NGAL (neutrophil gelatinase associated lipocalin) based on single-grain-size latex particles. The kit comprises a reagent R1 and a reagent R2; the reagent R1 is prepared from buffer solutions, surfactants, coagulants, chelating agents, preservatives, reductants, electrolytes and water; the reagent R2 is prepared from the buffer solutions, stabilizers, electrolytes and latex particles coated with antihuman NGAL polyclonal antibodies; the reductants are beta-mercaptoethanol; the antihuman NGAL polyclonal antibodies are biotin-labeled antihuman NGAL polyclonal antibodies; the latex particles are streptavidin-labeled single-grain-size latex particles. The kit has the advantages that NGAL in blood and urine samples can bedetected at the same time, so that the detection sensitivity is improved, the linear range is widened, and influences of interference factors can be avoided; NGAL homodimers and heterodimers are detected, and accordingly, misjudgment of doctors due to low NGAL detection results is avoided.