14results about How to "Wide detection linear range" patented technology

The invention relates to a preparation method of a Co-coated C / N composite material with a hollow super structure. Preparing a bimetal-centered ZIF material by using a coprecipitation method; a hollow super-structure ZIF material is obtained through selective etching; and performing high-temperature pyrolysis to obtain the hollow super-structure Co-coated C / N composite material. The invention also relates to a method for constructing a hydrogen peroxide electrochemical sensor by using the hollow super-structure Co-coated C / N composite material, the hollow super-structure Co-coated C / N material and Nafion are ultrasonically mixed and then dispensed on a working electrode, and the modified working electrode is obtained and used for constructing the hydrogen peroxide electrochemical sensor. The invention also relates to a method for detecting hydrogen peroxide using the hydrogen peroxide electrochemical sensor. The hollow super-structure Co (at) C / N composite material which is high in stability, good in conductivity, rich in active sites and controllable in morphology structure is conveniently prepared at low cost; the electron transmission rate of the material is improved; the sensitivity of the hydrogen peroxide electrochemical sensor is improved, the detection limit is reduced, and the detection linear range is widened.

The invention provides a monoclonal antibody for detecting alpha1-antitrypsin and application of the monoclonal antibody. The monoclonal antibody is any one of a monoclonal antibody A1 P39-5 and a monoclonal antibody A1 P40-6 or a combination of the monoclonal antibody A1 P39-5 and the monoclonal antibody A1 P40-6. The Serpin A1 antibody prepared by the invention has extremely high affinity, so that the detection sensitivity of a detection kit based on the antibody to Serpin A1 in serum reaches a pg / mL level which is far higher than a microgram / mL level of a conventional immunoturbidimetry. Meanwhile, the detection linear range is wide, a high-concentration sample does not need to be diluted for multiple times, and the operation error is reduced.

The invention belongs to the technical field of biology, and discloses a detection method of non-white proteinuria and application of the non-white proteinuria in auxiliary diagnosis of metabolic syndrome. The non-white proteinuria detection method comprises the following steps: S1, carrying out qualitative analysis on a urine sample to obtain qualitative detection results including urine pH, PRO and BLD; if the BLD is positive, judging that the urine sample is suspected non-white proteinuria; if the BLD is negative, executing the step S2; s2, according to the urine qualitative detection result, a urine sample quantitative detection mode is selected to carry out TPU and MALB quantitative detection on the urine sample, and a quantitative detection result is obtained; and jointly judging whether the urine sample is non-white proteinuria or not according to TPU and MALB quantitative detection results and PRO qualitative detection results. The invention also provides a non-white proteinuria detection system which comprises a laboratory information system, an IS system and a urine quantitative analysis system. The non-white proteinuria detection method can realize identification of white proteinuria and non-white proteinuria in proteinuria, and provides a new way for clinical diagnosis of MetS.

This invention relates to a hybridoma cell line, its secreted mouse anti-human Hb monoclonal antibody, and its applications, belonging to the fields of immunology and in vitro diagnostics. A hybridoma cell line is deposited at the China Center for Type Culture Collection (CCTCC), accession number CCTCC NO: C2023339. The hybridoma cell line prepared by this invention can stably secrete mouse anti-human Hb monoclonal antibodies. The mouse anti-human Hb monoclonal antibodies secreted by the hybridoma cell line have characteristics such as small batch-to-batch variability and high affinity. The detection kit prepared using the mouse anti-human Hb monoclonal antibody secreted by the hybridoma cell line of this invention has a wide linear range, high specificity, and good positive / negative differentiation.

The present invention discloses a fluorescent probe for detecting perchlorate in water, a preparation method and an application thereof. The present invention synthesizes a perylene core derivative with adjustable hydrophilicity at the molecular level as a fluorescent probe for perchlorate by modifying the perylene molecule and adding pyridine with a long alkyl group. By optimizing the carbon chain length, a strong selective recognition of perchlorate is achieved, and the detection limit can reach 6.37nM (0.63ug / L), which is much lower than the limit of 70ug / L. Experiments have shown that the fluorescent probe has high selectivity and high sensitivity for perchlorate. The detection method of the present invention has the characteristics of wide detection linear range, low detection limit, strong anti-interference ability, simple operation, high efficiency and speed, and low cost.

The invention belongs to the technical field of nano biology, and particularly relates to a double-resonance Raman scattering nano probe capable of being used for biomarker detection as well as a preparation method and application of the double-resonance Raman scattering nano probe. The nano probe is formed by compounding semiconductor nano particles such as ZnO, ZnS, ZnSe, ZnTe, CdS, CdSe, CdTe and the like. Resonance Raman scattering signals provided by the nanoprobe come from multi-order phonon Raman vibration peaks generated when semiconductor materials such as ZnO and ZnS are irradiated by specific exciting light, and the signals come from the self structure of a specified material, have unique and recognizable fingerprint characteristics and show excellent anti-interference capability. The nanoprobe prepared by the invention is relatively simple in preparation method and high in detection accuracy, can be adjusted according to actual requirements, realizes recognition and detection of various biomolecules, and has a wide application prospect in the field of biomedicine.

The invention discloses a label-free electrochemical aptamer sensor for detecting ochratoxin A as well as a construction method and application of the label-free electrochemical aptamer sensor. The sensor comprises a three-electrode system of a modified working electrode, a reference electrode and a counter electrode, and exonuclease I and potassium ferricyanide are contained in electrolyte; the modified working electrode is obtained by sequentially covalently immobilizing cDNA and an ochratoxin A aptamer on a VMSF modified electrode. According to the invention, potassium ferricyanide and an ochratoxin A aptamer are respectively used as an electrochemical signal molecule and a specific recognition element; a label-free electrochemical aptamer sensor is constructed by combining a signal amplification strategy of exonuclease I assisted target circulation, is used for detecting ochratoxin A in samples such as wheat samples and wine and has the characteristics of high sensitivity, wide linear range, simplicity in operation and the like.

The invention relates to the technical field of in-vitro detection, and discloses a human melatonin chemiluminescence immunodetection kit and a preparation method thereof.The human melatonin chemiluminescence immunodetection kit comprises a reagent R1 and a reagent R2, the reagent R1 comprises a magnetic bead coated melatonin antigen, and the reagent R2 comprises a melatonin antibody labeled by a chemiluminescence marker; simple reagent composition is used, expensive instruments and professional personnel are not needed, the cost is reduced, meanwhile, the detection sensitivity is improved, the detection linear range is expanded, and the stability is improved; sample analysis and detection can be automatically carried out through a detection system of a closed kit and instrument, system errors are reduced, errors caused by manual operation are eliminated, the detection efficiency is high, and the application prospect is wide.

The application discloses a preparation method of an electrochemical sensor for simultaneously detecting creatinine and uric acid, and relates to the technical field of medical detection. The technical scheme points of the application are as follows: ethanol, ethylene glycol and deionized water are uniformly mixed, then carbon nanotubes and anhydrous copper acetate are uniformly mixed with the mixture, and then the mixture is subjected to hydrothermal reaction, and after the reaction is completed and the mixture is cooled, the carbon nanotube / copper oxide / cuprous oxide nanocomposite is obtained by purification. In the application, the electrochemical sensor prepared by using the copper nanoparticle-related carbon nanocomposite can enhance the electron transmission capacity, thereby improving the electrochemical activity; meanwhile, the application can be used for detecting two biological molecules of creatinine and uric acid, has a low detection limit and a wide detection linear range, the creatinine detection limit is 0.002mM, the detection range is 0.01mM-1mM, the uric acid detection limit is 0.0004mM, and the detection range is 0.001mM-5mM.

The invention discloses a double-plasma metal synergistic metal organic framework composite SERS (Surface Enhanced Raman Scattering) substrate as well as a preparation method and application thereof in diazepam detection, and belongs to the technical field of drug detection. The SERS substrate is Ag (at) ZIF-8 (at) Au, and is specifically prepared by the following steps: mixing an Ag (at) ZIF-8 solution and an AuNPs solution, violently stirring, then standing for reaction, and obtaining the Ag (at) ZIF-8 (at) Au after the reaction is finished. The SERS substrate has good sensitivity, uniformity, reproducibility and storage stability and can be used for sensitive and efficient detection of diazepam, the detection limit is as low as 0.126 ng / mL, and the detection linear range is wide and reaches 10 <-2 >-10 <-9 > g / mL.

The invention belongs to the technical field of chlorogenic acid electrochemical sensors, and discloses a portable chlorogenic acid electrochemical sensor and a preparation method thereof, and the method comprises the following steps: S1, preparing a faint yellow powder solid Pd-ZIF-8; s2, weighing a finished product obtained in the step S1, and preparing TA-coated Pd-ZIF-8; s3, weighing a finished product in the step S2 to prepare a PdZn / NP-C dispersion liquid; s4, dropwise adding the dispersion liquid on the surface of a silk-screen printing working electrode to prepare a chlorogenic acid electrochemical sensor; the PdZn / NP coated C / SPE modified working electrode is utilized, and the portable workstation is adopted for testing, so that the problems of poor portability, higher minimum detection limit and narrow detection linear range of the existing detection method are solved.

The present invention relates to a method for detecting thrombopoietin receptor agonists in biological samples, comprising the steps of: mixing a test sample with an internal standard precipitant, vortexing to obtain a supernatant of the test sample, and using ultra-high performance liquid chromatography coupled to mass spectrometry to quantitatively detect the thrombopoietin receptor agonist in the supernatant of the test sample; the internal standard precipitant contains an internal standard and a special solvent, the internal standard is an isotope internal standard of the thrombopoietin receptor agonist, the special solvent contains methanol, acetonitrile and ammonia water, and the content of the components in the special solvent is: methanol> ammonia water, acetonitrile> ammonia water. The advantages of the present invention are: the special solvent provided can stably and fully dissolve the thrombopoietin receptor agonist standard and the isotope internal standard, is friendly to instrument accessories, can precipitate the protein in the plasma, and reduce the sample pre-treatment time; the thrombopoietin receptor agonist detection method provided has accurate analysis, a wide detection linear range, and a short detection time.

The invention discloses an electrochemical sensor as well as a preparation method and application thereof, and relates to the technical field of sensors. The electrochemical sensor comprises a working electrode, a reference electrode and a counter electrode, wherein the surface of the working electrode of the electrochemical sensor contains a nano composite material; the nano composite material comprises a nitrogen-doped carbon-coated ferric oxide material and conductive nano particles. The electrochemical sensor disclosed by the invention has high sensitivity and high stability, has strong adsorption capacity on a target object when being applied to the field of detection, is small in relative standard deviation of test results obtained after being placed for different days, is particularly suitable for detection of 2, 4-dichlorophenol, and has the advantages of wide detection linear range, high sensitivity and the like.

The invention discloses a preparation method of a zinc modified mesoporous Y type molecular sieve. The preparation method comprises the following steps: preparing a mesoporous NaY type molecular sieve by taking a Bola type surfactant [C5H5-N < + >-(CH2) 10-O-(p-C6H4) 2-O-(CH2) 10-N < + >-C5H5]. 2 [Br <->] as a template agent and matching with a specific raw material proportion; the NaY type molecular sieve is sequentially soaked in an ammonium salt solution and a zinc salt solution for ion exchange, and after drying and calcination, the zinc modified mesoporous Y type molecular sieve is obtained. The zinc-modified mesoporous Y-type molecular sieve prepared by the invention has larger specific surface area, higher pore volume and excellent conductivity, the detection range of the zinc-modified mesoporous Y-type molecular sieve applied to an electrochemical sensor for detecting the content of acetaminophen is 0.066-10000 [mu] mol / L, the detection limit can reach 0.01 [mu] mol / L, meanwhile, the zinc-modified mesoporous Y-type molecular sieve has excellent detection sensitivity, stability, reproducibility and selectivity, and the application prospect is wide. Accurate determination of acetaminophen in drugs can be achieved, and the prepared electrochemical sensor is simple in structure, convenient to operate and low in detection cost and has wide application prospects.