4results about How to "Wide detection linear range" patented technology

The invention provides a monoclonal antibody for detecting alpha1-antitrypsin and application of the monoclonal antibody. The monoclonal antibody is any one of a monoclonal antibody A1 P39-5 and a monoclonal antibody A1 P40-6 or a combination of the monoclonal antibody A1 P39-5 and the monoclonal antibody A1 P40-6. The Serpin A1 antibody prepared by the invention has extremely high affinity, so that the detection sensitivity of a detection kit based on the antibody to Serpin A1 in serum reaches a pg / mL level which is far higher than a microgram / mL level of a conventional immunoturbidimetry. Meanwhile, the detection linear range is wide, a high-concentration sample does not need to be diluted for multiple times, and the operation error is reduced.

This invention relates to a hybridoma cell line, its secreted mouse anti-human Hb monoclonal antibody, and its applications, belonging to the fields of immunology and in vitro diagnostics. A hybridoma cell line is deposited at the China Center for Type Culture Collection (CCTCC), accession number CCTCC NO: C2023339. The hybridoma cell line prepared by this invention can stably secrete mouse anti-human Hb monoclonal antibodies. The mouse anti-human Hb monoclonal antibodies secreted by the hybridoma cell line have characteristics such as small batch-to-batch variability and high affinity. The detection kit prepared using the mouse anti-human Hb monoclonal antibody secreted by the hybridoma cell line of this invention has a wide linear range, high specificity, and good positive / negative differentiation.

The application discloses a preparation method of an electrochemical sensor for simultaneously detecting creatinine and uric acid, and relates to the technical field of medical detection. The technical scheme points of the application are as follows: ethanol, ethylene glycol and deionized water are uniformly mixed, then carbon nanotubes and anhydrous copper acetate are uniformly mixed with the mixture, and then the mixture is subjected to hydrothermal reaction, and after the reaction is completed and the mixture is cooled, the carbon nanotube / copper oxide / cuprous oxide nanocomposite is obtained by purification. In the application, the electrochemical sensor prepared by using the copper nanoparticle-related carbon nanocomposite can enhance the electron transmission capacity, thereby improving the electrochemical activity; meanwhile, the application can be used for detecting two biological molecules of creatinine and uric acid, has a low detection limit and a wide detection linear range, the creatinine detection limit is 0.002mM, the detection range is 0.01mM-1mM, the uric acid detection limit is 0.0004mM, and the detection range is 0.001mM-5mM.

The invention discloses an electrochemical sensor as well as a preparation method and application thereof, and relates to the technical field of sensors. The electrochemical sensor comprises a working electrode, a reference electrode and a counter electrode, wherein the surface of the working electrode of the electrochemical sensor contains a nano composite material; the nano composite material comprises a nitrogen-doped carbon-coated ferric oxide material and conductive nano particles. The electrochemical sensor disclosed by the invention has high sensitivity and high stability, has strong adsorption capacity on a target object when being applied to the field of detection, is small in relative standard deviation of test results obtained after being placed for different days, is particularly suitable for detection of 2, 4-dichlorophenol, and has the advantages of wide detection linear range, high sensitivity and the like.