Construction and application of multifunctional targeted drug delivery system taking nano-diamond as carrier

A nano-diamond and targeted drug delivery technology, which is applied in the field of cancer treatment, achieves the effect of simple and convenient synthesis method and reduced side effects

Active Publication Date: 2018-04-03
TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, previous studies on Raman imaging scanning methods in this field are based on point scanning superposit

Method used

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  • Construction and application of multifunctional targeted drug delivery system taking nano-diamond as carrier
  • Construction and application of multifunctional targeted drug delivery system taking nano-diamond as carrier
  • Construction and application of multifunctional targeted drug delivery system taking nano-diamond as carrier

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] 1) Nano-diamond homogenization

[0060] Add 0.5g of nano-diamond powder into the mixed acid of sulfuric acid and nitric acid (the volume ratio of sulfuric acid and nitric acid is 3:1), stir at 70°C for 24h to obtain the first mixture; cool the first mixture to room temperature, suck out For excess acid in the first mixture, add 10 mL of 0.1 mol / L aqueous sodium hydroxide solution to the first mixture, stir at 70°C for 60 min to obtain a second mixture; put the second mixture on stand , and then use a straw to absorb the supernatant and measure its pH value, wash the second mixture several times to make the pH value neutral, and obtain carboxylated nano-diamonds with a uniform surface.

[0061] 2) Covalent binding of cetuximab

[0062] Disperse the carboxylated nano-diamond in water and perform ultrasonication for 10 min to obtain a carboxylated nano-diamond aqueous solution with a concentration of 1 mg / mL; take 100 μL of a carboxylated nano-diamond aqueous solution wit...

Embodiment 2

[0070] 1) Nano-diamond homogenization

[0071]Add 0.5g of nano-diamond powder into the mixed acid of sulfuric acid and nitric acid (the volume ratio of sulfuric acid and nitric acid is 2:1), stir at 70°C for 12h to obtain the first mixture; cool the first mixture to room temperature, suck out For excess acid in the first mixture, add 10 mL of 0.1 mol / L aqueous sodium hydroxide solution to the first mixture, stir at 70°C for 60 min to obtain a second mixture; put the second mixture on stand , and then use a straw to absorb the supernatant and measure its pH value, wash the second mixture several times to make the pH value neutral, and obtain carboxylated nano-diamonds with a uniform surface.

[0072] 2) Covalently bound folic acid

[0073] Disperse the carboxylated nano-diamond in water and perform ultrasonication for 10 min to obtain a carboxylated nano-diamond aqueous solution with a concentration of 5 mg / mL; take 100 μL of a carboxylated nano-diamond aqueous solution with a...

Embodiment 3

[0077] 1) Nano-diamond homogenization

[0078] Add 0.5g of nano-diamond powder into the mixed acid of sulfuric acid and nitric acid (the volume ratio of sulfuric acid and nitric acid is 4:1), stir at 70°C for 18h to obtain the first mixture; cool the first mixture to room temperature, suck out For excess acid in the first mixture, add 10 mL of 0.1 mol / L aqueous sodium hydroxide solution to the first mixture, stir at 70°C for 60 min to obtain a second mixture; put the second mixture on stand , and then use a straw to absorb the supernatant and measure its pH value, wash the second mixture several times to make the pH value neutral, and obtain carboxylated nano-diamonds with a uniform surface.

[0079] 2) Covalently bind transferrin

[0080] Disperse the carboxylated nano-diamond in water and perform ultrasonication for 10 min to obtain a carboxylated nano-diamond aqueous solution with a concentration of 3 mg / mL; Add 100 μL of 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (ED...

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Abstract

The invention discloses construction and an application of a multifunctional targeted drug delivery system taking nano-diamond as a carrier. Firstly, the novel nano-diamond multifunctional targeted drug delivery system is offered, wherein in the system, the nano-diamond is taken as the carrier; the nano-diamond is bonded with targeting molecules in a covalent mode, so that targeting molecule-nano-diamond is formed, and the targeting molecule-nano-diamond is loaded with an anti-cancer drug in a non-covalent mode, so that a targeting molecule-nano-diamond-anti-cancer drug is formed. The invention also provides a synthesis method of the system, wherein the method is simple and easy to operate. Furthermore, the invention provides a three-dimensional Raman imaging technique applied to a visualnano-diamond drug delivery system and a cancer cell acting process; and precise localization of tumors can be implemented, cancer cells can be effectively killed and the visualization of an endocytosis process can be realized; therefore, the drug delivery system has an important significance for tumor treatment.

Description

technical field [0001] The present invention relates to the field of cancer therapy. More specifically, it relates to the construction and application of a multifunctional targeted drug delivery system with nanodiamond as a carrier. Background technique [0002] With the advancement of medical technology and the development of material science, the advantages of drug delivery systems in cancer treatment are gradually revealed. At present, anti-cancer drug delivery systems based on liposomes and polymer materials have been used in a large number of clinical applications. These systems have excellent performance in cancer treatment, and the cost is almost the same as that of ordinary drugs. Among the widely used drug delivery systems, some of them are loaded with targeting molecules and ligands in order to better achieve targeted drug delivery to cancer cells, and the clinical performance of these multifunctional targeted drug delivery systems is particularly excellent. Howe...

Claims

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Application Information

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IPC IPC(8): A61K47/68A61K47/64A61K47/54A61K33/24A61K31/704A61K31/337A61P35/00G01N21/65
CPCA61K31/337A61K31/704A61K33/24G01N21/65
Inventor 只金芳李丹丹陈鑫
Owner TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
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