86 results about "Chlorin e6" patented technology

Siderophore-photosensitizer conjugates, their synthesis and use in photodynamic antimicrobial therapy (PACT) is disclosed. The advantage of this method is improvement of photodynamic antimicrobial therapy against, for example, pathogenic micro-organisms such as bacteria and fungi. Naturally occurring and synthetically available siderophore structures are conjugated chemically with photoactive compounds such as Chlorin e6 to improve their penetration into bacterial cells and to increase antibacterial efficacy of photosensitizers via microbial proteins that recognize and transport iron-loaded siderophores. In this way, photosensitizers can be transported inside bacteria that otherwise could not cross the cell wall and membranes. Photodynamic activation of photosensitizers inside the cells of pathogenic microbes enables a more effective inhibition of cellular functions than application at the outer side of the cells. The siderophore-transporting systems of microbes are known to be specific for bacteria and fungi. Consequently, siderophore conjugates with photosensitizers are not taken up by mammalian cells and photodynamic effects can thus be exerted specifically on pathogenic microbes. Applications of these conjugates include highly efficient treatment of pathogenic gram-negative and -positive bacteria such as Pseudomonas aeruginosa, Escherichia coli, Streptococcus pyogenes, Staphylococcus aureus, treatment of microbial infections that often occur in chronic wounds as well as therapy of other antibiotic resistant microbial infections.

The invention provides thermo-sensitive liposome. The thermo-sensitive liposome comprises a chemotherapeutic drug, a photosensitizer, phosphatidylcholine and polyethylene glycol-derived phospholipid, wherein the photosensitizer is indocyanine green or chlorins e6; the grain size of the thermo-sensitive liposome is 40-60 nm; by virtue of living-body fluorescence imaging, a distribution process of the thermo-sensitive liposome can be observed in real time; under a near-infrared light irradiation condition, the chemotherapeutic drug can be quickly and effectively released, so that the chemotherapeutic drug can be enriched at a target part very well; the obtained thermo-sensitive liposome particle has smaller particle diameter less than 100 nm, and good biocompatibility. The preparation method of the thermo-sensitive liposome is simple and easy to operate.

The invention belongs to the technical field of biological medicine, and relates to a lipidosome preparation, and a preparation method and application thereof, in particular to lipidosome-dimethyldiguanide-chlorin e6, and preparation and application thereof, particularly application to tumor photodynamic treatment and radiotherapy. The lipidosome preparation (lipidosome-dimethyldiguanide-chlorin e6) provided by the invention consists of a lipidosome, dimethyldiguanide and chlorin e6; the particle size is uniform; the dispersion in water is good; the particle size in water is mainly about 110nm; photosensitive molecules and the dimethyldiguanide can be simultaneously loaded; the lipidosome preparation is an ideal medicine carrier and has good stability. Experiments show that the lipidosome-dimethyldiguanide-chlorin e6 can be highly enriched in the tumor position; a continuous effect is achieved for lowering the tumor cell oxygen consumption quantity; the anoxia condition of the tumor position can be obviously improved; a good photodynamic treatment and radiotherapy effect is achieved.

The invention relates to a chlorin and artesunate compound with photosensitive and sound-sensitive activity as well as a preparation method and an application of the compound and belongs to the technical field of chemical medicines. The chlorin and artesunate compound with photosensitive and sound-sensitive activity has different degrees of inhibition effects on human hepatoma cells Hep G2 in in-vitro anti-tumor activity evaluation. The photoactivity and ultrasonic activity are both higher than that of chlorin e6 and artesunate which are used as positive controls. The compound can be used for preparing a photosensitizer and a sound-sensitive agent in photodynamic therapy and sonodynamic therapy methods for tumor therapy.

The invention provides a preparation of chlorin e6 chitosan-stearic acid graft micelle. The preparation comprises a chitosan-stearic acid graft and chlorin e6, wherein, the chlorin e6 accounts for 4.76-16.76% of the total weight; the chitosan-stearic acid graft is formed by chemically grafting low molecular weight chitosan with average molecular weight being 18.0 KDa and stearic acid; and the amino substituted ration of the chitosan-stearic acid graft accounts for 4.96%. The invention utilizes the chitosan-stearic acid graft micelle to effectively pack the photosencitizer of chlorin e6, so as to prepare the intracellular administration preparation of chlorin e6 chitosan-stearic acid graft micelle. Compared with the chlorin e6 solution, the preparation of the invention can significantly improve the drug concentration of chlorin e6 in tumour cells and provide probability for the follow-up realization of efficient and harmless photodynamic therapy of tumors.

The invention relates to the technical field of biological medicines, and in particular relates to a protein-polypyrrole compound as well as a preparation method and application of a protein-polypyrrole compound derivative. The pyrrole in the protein-polypyrrole compound provided by the invention is wrapped on the surface of protein in an in situ polymerization manner. The protein-polypyrrole compound can generate very high heat under illumination of laser after being swallowed by cells, and can kill tumor cells, so that the protein-polypyrrole compound is a good photo-thermal reagent and can be used as a carrier for carrying a photosensitizer. The pyrrole in the protein-polypyrrole compound derivative provided by the invention is wrapped on the surface of the protein carrying chlorin e6 in an in situ polymerization manner, and is an ideal photo-thermal therapeutic agent, a photodynamic therapeutic agent or a photo-thermal and photodynamic combined therapeutic agent. Moreover, because chlorin e6 can be used for fluorescence imaging of tumors, the invention also discloses an application of the protein-polypyrrole compound derivative serving as an imaging agent of the tumors.

The present invention relates to a novel chlorin e6-folic acid conjugate, a preparation method thereof, and a pharmaceutical composition for the treatment of cancer comprising the same, and more particularly, to a novel compound prepared by linking chlorin e6 to folic acid, which effectively produces singlet oxygen in various media and has much better tumor selectivity than the known porphyrin-based photosensitizers, thereby being used effectively in photodynamic therapy for malignant tumors, a preparation method thereof, and a pharmaceutical composition for photodynamic treatment of solid tumors comprising the compound.

The invention discloses an antibacterial material with pH responsivity and light sensitivity and a preparation method thereof. Hydrophilic and hydrophobic photodynamic molecules are loaded in a nano particle polymer Poly(HDDA-co-DBPA)-PEG. High molecular polymer nano particles with pH responsivity are prepared and are subjected to hydrophilic PEG modification, and photodynamic molecule medicines Chlorin e6 are loaded into the nano particles by hydrophilic and hydrophobic properties. Multiple biological antibacterial technologies are integrated, the bactericidal biological effect is increased, the adhesion of medicines in special environments is effectively increased, the bacterial organism growth is inhibited, an effective antibacterial effect can be achieved, the biocompatibility is good, the cost is low, and the material stability is good. A biological macromolecular antibacterial material obtained by the preparation method disclosed by the invention has the characteristics of effective antibacterial effect, good biocompatibility, good stability and low cost.

The invention belongs to the technical field of medicine and pharmacy, and relates to pure photosensitizer self-assembled nanoparticles so as to achieve the effects of being high in medicine loading quantity, good in stability and low in toxic and side effects, and capable of performing specific disintegration on tumor locations, retarding aggregation-caused quenching (ACQ) effects, and improvingantitumor activity. The pure photosensitizer self-assembled nanoparticles provided by the invention are prepared through separate self-assembly of a photosensitizer, or prepared through self-assemblyof the photosensitizer and a PEG modifier through core-sheath matching, wherein the photosensitizer is one or more of pheophorbide a, chlorophyll a, pheophorbide a, 2-[1-hexyloxyethyl]-2-devinyl pyropheophorbide-a and chlorin e6, the PEG modifier is an amphiphilic polymer of an amphipathic PEG modifier, PEG and the photosensitizer, and the weight ratio of the photosensitizer to the PEG modifier is(10:0.5)-(10:3). A new strategy and more choices are provided for developing a pure medicine self-assembled delivery system, and urgent requirements for efficient chemotherapy preparations in clinical application are met.

An optochemical sensor element suitable for sensing an analyte comprises a polymeric material in which at least a portion of the polymer material is solvent crazed to provide a multiplicity of pores of controlled nanometer size, and an indicator dye impregnated into the pores, in which optochemical sensor element the indicator dye is a long-decay photoluminescent dye selected from the group consisting of: phosphorescent platinum(II)—and palladium (II) complexes of porphyrin dyes such as octaethylporphine, coproporphyrin, octaethylporphine -ketone, benzoporphine, tetra (pentafluorophenyl) porphine, chlorin e6; fluorescent complexes of ruthenium (II), osmium(II), iridium(III) and europium (III); or derivatives or close analogs of these dyes. The sensor element in the presence of the analyte alters a photoluminescence parameter thus allowing sensing and/or quantification of the analyte.

The invention belongs to the field of medical chemistry and particularly relates to a preparation method for Talaporfin and an intermediate thereof. The Talaporfin is obtained by taking a compound chlorins e6 mono aspartic acid-4-ester as a key intermediate and hydrolyzing the chlorins e6 mono aspartic acid-4-ester. The preparation method for the Talaporfin and the intermediate thereof has the advantages that the product purity is improved; the operation is simplified; requirements on equipment are lowered; and industrial production is easy.

The invention discloses a chlorins glucoside compound, and a preparation method and application thereof, and belongs to the technical field of chemical medicines. According to the chlorins glucoside compound disclosed by the invention, through the selective combination of the glucose transport protein which is highly expressed on the surface of the tumor cells, the targeting effect of a photosensitizer and a sound-sensitive agent on tumor cells is improved. The in-vitro anti-tumor activity evaluation shows that compared with chlorins e6 which is used as a control, the chlorins glucoside compound provided by the invention has relatively high light activity and sound activity on humanhepatoma carcinoma cells Hep G2. The chlorins glucoside compound disclosed by the invention can be used for preparation of a photosensitizer and a sound-sensitive agent in photodynamic therapy and sonodynamics therapy methods for tumor therapy. According to the invention, the preparation process is simple, the reaction conditions are easy to control, and the production is facilitated.

The invention relates to tyroserleutide-chlorin e6 monoester prepared by esterification of the tyroserleutide with the chlorin e6. With the tyroserleutide as a water soluble polar end, the tyroserleutide-chlorin e6 monoester can dissolve in water. Consequently, the tyroserleutide-chlorin e6 monoester has the advantages of solving the problem of poor water solubility of Ce6, having double antitumoreffects of the chlorin e6 and the tyroserleutide, being to photodynamic and sonodynamic therapeutic drugs, and being applicable to prepare drugs for treating skin diseases and malignant tumors.

The invention relates to a preparation method and application of phycocyanin-chlorin e6 covalent nanoparticles, and belongs to the technical field of medical functional materials. The preparation method comprises the following steps: firstly performing activation on chlorin e6 at normal temperature, adding the activated chlorin e6 dropwise into a phycocyanin solution dissolved in PBS, performing areaction for a period of time, taking out the solution, performing centrifugation, performing dialysis, and performing lyophilization to obtain the powder. The method uses phycocyanin to target tumorcells and uses laser as a light source, irradiation is performed after a photosensitizer is enriched in tumors, so that the output of active oxygen in photodynamic therapy can be greatly improved, tumor growth can be inhibited, and the therapeutic effect is significantly enhanced.

The invention discloses a branched polyetherimide material containing a ketone thioacetal bond, as well as a preparation method and application thereof. The preparation method comprises the followingsteps: (1) stirring acetone and acid containing thiol to react at room temperature; (2) adding polyetherimide and an activator after the reaction is finished, dissolving in an organic reagent, stirring to react, bonding chlorin e6 and carboxyl polyethylene glycol through an amidation reaction to obtain the reactive oxygen sensitive branched polyetherimide material containing the ketone thioacetalbond. The branched polyetherimide material has excellent biocompatibility and degradability, is entrapped with siRNA, and forms nano-particles by self-assembly, can generate a great number of reactiveoxygen under excitation of a light source with specific wavelength to destroy the structure of endosome, the reactive oxygen sensitive ketone thioacetal bond is broken, particles are disintegrated, and endosome escape and release of intracellular siRNA can be accelerated. The branched polyetherimide material containing a ketone thioacetal bond has a huge clinical application potential in the field of tumor treatment.

The invention relates to a chlorins e6 metal salt compound.The chemical structure is shown in the formula (I) in the description, wherein R is common metal ions such as K+ or Na+.A preparing method comprises the following steps that firstly, silkworm excrement crude product chlorophyll serves as a raw material and is dissolved in diethyl ether, concentrated hydrochloric acid is added, and magnesium-removed chlorophyllin a is generated; secondly, magnesium-removed chlorophyllin a is reacted in a strong-base methanol solution, a little of water is added later to generate chlorins e6 mono-methyl ester; thirdly, obtained chlorins e6 mono-methyl ester is reacted in a strong-base alcoholic solution reaction reagent, and a compound (I) is obtained through separation and purification.The chlorins e6 metal salt compound is a raw material in the study field of medicine for resisting gastric ulcers, resisting anemia and protecting the liver and promoting leucopoiesis.

The invention provides a nanocarrier medicine, as well as a preparation method and an application of the nanocarrier medicine. The nanocarrier medicine takes polylactic acid-glycolic acid as a carrier, and is loaded with NVP-BEZ235 and chlorin E6, and the surface of a carrier is subjected to PEGylation by using lecithin and distearoyl phosphoethanolamine-polyethylene glycol. The polylactic acid-glycolic acid is taken as the carrier, the chlorin E6 and the NVP-BEZ235 are combined to serve as a therapeutic agent, and the photodynamics therapy and the biotherapy are combined and produce a synergistic effect to improve the killing effect on a tumor cell. The lecithin and the DSPE-PEG are used to conduct PEGylation on the surface of a nanoparticle, the time for blood circulation is prolonged, the clearing of reticuloendothelium is reduced, the therapeutic effect is further guaranteed, so that the therapeutic effect of tumors, particularly the triple negative breast cancer, is obvious, and the nanocarrier medicine has a broad application prospect.

The invention provides a preparation method of a chitosan-chlorin e6 antibacterial agent. In the chitosan-chlorin e6 antibacterial agent, chlorin e6 accounts for 13.48%-22.04% of the total weight of the antibacterial agent; chitosan-chlorin e6 is obtained by combining chitosan and the chlorin e6 through an amido bond; and the amino substitution degree of the chlorin e6 to the chitosan in the chitosan-chlorin e6 is 6.44%-11.68%. According to the invention, the chitosan is used as a carrier of a photosensitizer, namely the chlorin e6, and the chitosan-chlorin e6 antibacterial agent is prepared. Compared with the chlorin e6, the antibacterial agent disclosed by the invention has the advantages that the antibacterial activity of the chlorin e6 on staphylococcus aureus and escherichia coli can be effectively improved, and the antibacterial agent has application value in various fields including agriculture, medicine and the like.

The invention provides a chlorin e6 ferrocene conjugate with photo- and acoustic-sensitive activity as well as a preparation method and application thereof, and belongs to the technical field of chemical medicines. The chlorin e6 ferrocene conjugate disclosed by the invention has different degrees of inhibition effects on Hela cells in in-vitro anti-tumor activity evaluation. The introduction of the ferrocene group significantly improves the proliferation inhibition activity of the compound on tumor cells, and the proliferation inhibition activity of the chlorin e6 ferrocene conjugate on tumorcells is much higher than that of chlorin e6 used as a control group. The method can be used for preparing photosensitizers and sonosensitizers in photodynamic therapy and sonodynamic therapy methodsfor tumor therapy.

The present invention relates to a pharmaceutical composition or a cosmetic composition, comprising, as an active ingredient, chlorin e6 having excellent antibacterial activity against Propionibacterium acnes.