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Preparation and application of phycocyanin-chlorin e6 covalent nanoparticles

A technology of phycocyanin and chlorin, which is applied in the direction of medical preparations with non-active ingredients, medical preparations containing active ingredients, wave energy or particle radiation treatment materials, etc., which can solve unfavorable cell uptake, poor water solubility, etc. problems, to achieve the effect of simple and easy operation of equipment and process, less pollution of reagents and remarkable curative effect

Inactive Publication Date: 2020-06-09
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, Ce6 is a photosensitizer approved by the FDA and can be used clinically. It has a simple structure and strong absorption at 660 nm, but its water solubility is poor, which is not conducive to cell uptake.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0033] A preparation method of phycocyanin-chlorin e6 covalent nanoparticles, the specific operation steps are as follows:

[0034] (1) Activated chlorin e6

[0035] Weigh a certain amount of Ce6, N-hydroxysuccinimide, and 1-ethyl-3-(3-dimethylpropylamine) carbodiimide into the DMSO solution, ultrasonically dissolve it fully, and keep it in the dark at room temperature Stir and activate for a period of time to obtain Ce6 derivatives;

[0036] (2) Preparation of phycocyanin solution

[0037] According to different molar ratios of Ce6:phycocyanin, several phycocyanins were weighed and dissolved in PBS, ultrasonically dissolved, centrifuged, the supernatant was taken to measure the electronic absorption spectrum, and the concentration of phycocyanin was quantified;

[0038] (3) Prepare phycocyanin-chlorin e6 solution;

[0039] Take out the activated Ce6 derivative, add it to the stirring phycocyanin solution, and react overnight;

[0040] (4) preparing phycocyanin-chlorin e6 ...

Embodiment 1

[0050] Dilute the phycocyanin-Ce6 20 solution, add an appropriate amount of 50% (volume fraction) glutaraldehyde to fix the glutaraldehyde concentration in the solution to 2% for 2 h, pipette 10 μl of the solution on the carbon support membrane, and place The copper grid was dried in an oven at 30°C for 12 hours, and then photographed with an ultra-high resolution field emission scanning electron microscope. The particle size is about 140nm.

Embodiment 2

[0052] Add 100 μL of the prepared 2,7-dichlorodihydrofluorescein solution to the solution to be tested to make the final concentration 20 μM. 1 W / cm with 635 nm laser 2 , illuminate the above solution, measure the fluorescence once every 10 s (excitation wavelength: 488 nm), and illuminate for 60 s in total. The greater the change in fluorescence intensity at 525nm in the fluorescence spectrum, the stronger the ability to generate ROS. After the aqueous solution containing only 2,7-dichlorodihydrofluorescein was illuminated for 60 s, its fluorescence intensity at 525 nm increased by 3 times, which was because 2,7-dichlorodihydrofluorescein was oxidation. The fluorescence intensity of 2,7-dichlorodihydrofluorescein solution containing free Ce6 increases with the increase of illumination time, indicating that Ce6 generates ROS under illumination, and its fluorescence intensity at 525 nm after illumination for 60s is 1.5×10 6 , increased by about 64 times relative to the initi...

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Abstract

The invention relates to a preparation method and application of phycocyanin-chlorin e6 covalent nanoparticles, and belongs to the technical field of medical functional materials. The preparation method comprises the following steps: firstly performing activation on chlorin e6 at normal temperature, adding the activated chlorin e6 dropwise into a phycocyanin solution dissolved in PBS, performing areaction for a period of time, taking out the solution, performing centrifugation, performing dialysis, and performing lyophilization to obtain the powder. The method uses phycocyanin to target tumorcells and uses laser as a light source, irradiation is performed after a photosensitizer is enriched in tumors, so that the output of active oxygen in photodynamic therapy can be greatly improved, tumor growth can be inhibited, and the therapeutic effect is significantly enhanced.

Description

technical field [0001] The present invention relates to a phycocyanin-chlorin e6 covalent nanoparticle for photodynamic therapy of tumors and its application, specifically, the present invention relates to a phycocyanin-chlorin covalent nanoparticle The combined reagent can be used in the method of photodynamic therapy for tumors, and uses phycocyanin as a carrier to enhance the effect of photodynamic therapy, so as to inhibit the growth of tumors. Background technique [0002] There are many treatment methods for tumors, but at present the most basic and main methods are still surgery, radiotherapy and chemotherapy. Exploring new treatment methods with less toxic and side effects, or adding some auxiliary means on the basis of the current treatment methods, so as to improve the curative effect and reduce the toxic and side effects, is an urgent problem to be solved in the current tumor treatment. [0003] In recent years, photodynamic therapy has attracted widespread atten...

Claims

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Application Information

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IPC IPC(8): A61K41/00A61K9/14A61K47/64A61P35/00
CPCA61K9/146A61K41/0071A61P35/00
Inventor 黄剑东祝登辉林晨张雅
Owner FUZHOU UNIV
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