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Preparation method of chlorin e6 and ferroferric oxide composite nanoparticle

A composite nanoparticle and ferric oxide technology, which is applied in nanotechnology, nanotechnology, nanomedicine, etc., can solve the problem of poor water solubility of photosensitizer chlorin e6, increase water solubility of photosensitizer chlorin e6, increase Problems such as blood circulation stability and dispersibility, to achieve the effect of increasing blood circulation stability and dispersibility, increasing water solubility, and simple preparation method

Inactive Publication Date: 2018-09-28
AFFILIATED HOSPITAL OF GUILIN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The present invention solves the problem of poor water solubility of the photosensitizer chlorin e6 in the prior art without changing the structure or function, and increases the water solubility of the photosensitizer chlorin e6, thereby increasing the Circulation Stability and Dispersion

Method used

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  • Preparation method of chlorin e6 and ferroferric oxide composite nanoparticle
  • Preparation method of chlorin e6 and ferroferric oxide composite nanoparticle
  • Preparation method of chlorin e6 and ferroferric oxide composite nanoparticle

Examples

Experimental program
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Effect test

Embodiment 1

[0046] The preparation method of the chlorin e6 of the present embodiment and ferric oxide composite nanoparticle, comprises the steps:

[0047] Step 1: Preparation of oleic acid-coated Fe3O4 nanoparticles

[0048] Take 18mL of octadecene, add it to a 50mL three-necked bottle, blow in nitrogen, and then add 0.8mmol FeCl 2 Solution, 4mmol oleic acid and 4mmol oleylamine, heated to 100-120°C, stay for 1h; then add 1.8mmol Fe(acac) 3 solution, heated to 180°C, and stayed for 30 minutes; continued to heat to 280°C, stayed for 30 minutes, and then stopped the nitrogen to obtain crude ferric oxide nanoparticles coated with oleic acid;

[0049] Cool the crude iron ferric oxide nanoparticles coated with oleic acid to room temperature, add 50 mL of absolute ethanol for magnetic separation, then add 20 mL of acetone, centrifuge, take the precipitate, dissolve it in 20 mL of chloroform, and obtain pure Ferric oxide nanoparticles coated with oleic acid;

[0050] Step 2: Preparation of ...

Embodiment 2

[0053] Step 1: Preparation of oleic acid-coated Fe3O4 nanoparticles

[0054] Take 20mL of octadecene, add it to a 50mL three-necked bottle, blow in nitrogen, and then add 1.0mmol FeCl 2 Solution, 6mmol oleic acid and 6mmol oleylamine, heated to 100-120°C, stayed for 1h; then added 2.0mmol Fe(acac) 3 solution, heated to 200°C, and stayed for 30 minutes; continued heating to 290°C, and stayed for 30 minutes, then stopped the nitrogen gas to obtain crude ferric oxide nanoparticles coated with oleic acid;

[0055] Cool the crude iron ferric oxide nanoparticles coated with oleic acid to room temperature, add 75mL absolute ethanol, carry out magnetic separation, then add 35mL acetone, centrifuge, take the precipitate, dissolve it in 35mL chloroform, and obtain pure Ferric oxide nanoparticles coated with oleic acid;

[0056] Step 2: Preparation of composite nanoparticles of chlorin e6 and ferric oxide

[0057] Weigh 50 mg of distearoylphosphatidylethanolamine-polyethylene glycol 2...

Embodiment 3

[0059] Step 1: Preparation of oleic acid-coated Fe3O4 nanoparticles

[0060] Take 22mL of octadecene, add it to a 50mL three-neck bottle, blow in nitrogen, and then add 1.2mmol FeCl 2 solution, 7mmol oleic acid and 7mmol oleylamine, heated to 120°C and stayed for 1h; then added 2.2mmol Fe(acac) 3 solution, heated to 180-220°C, and stayed for 30 minutes; continued to heat to 300°C, and stayed for 30 minutes, then stopped the nitrogen gas to obtain crude ferric oxide nanoparticles coated with oleic acid;

[0061] Cool the crude iron ferric oxide nanoparticles coated with oleic acid to room temperature, add 100mL of absolute ethanol, perform magnetic separation, then add 50mL of acetone, centrifuge, take the precipitate, dissolve it in 50mL of chloroform, and obtain pure Ferric oxide nanoparticles coated with oleic acid;

[0062] Step 2: Preparation of composite nanoparticles of chlorin e6 and ferric oxide

[0063] Weigh 60 mg of distearoylphosphatidylethanolamine-polyethylene...

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Abstract

The invention discloses a preparation method of a chlorin e6 and ferroferric oxide composite nanoparticle and belongs to the technical field of photosensitizer nanocrystallization. The preparation method of the chlorin e6 and ferroferric oxide composite nanoparticle comprises the following steps of preparing ferroferric oxide nanoparticles coated with oleic acid; preparing the chlorin e6 and ferroferric oxide composite nanoparticle. Under the condition of not changing the structure and the functions, the preparation method of the chlorin e6 and ferroferric oxide composite nanoparticle solves the problem of poor water solubility of the photosensitizer of chlorin e6 in the prior art, enhances the water solubility of the photosensitizer of chlorin e6 and further improves blood circulation stability and dispersibility. Meanwhile, the preparation method of the chlorin e6 and ferroferric oxide composite nanoparticle can enhance the superparamagnetism of the photosensitizer of chlorin e6.

Description

technical field [0001] The invention relates to a method for preparing composite nanoparticles of chlorin e6 and ferric oxide, and belongs to the technical field of photosensitizer nanotechnology. Background technique [0002] Photodynamic therapy (PDT) is a new tumor treatment technology under research and development, and it has shown a good application prospect in the diagnosis and prevention of tumors. Compared with traditional chemotherapy, radiotherapy, surgery and other treatment methods, photodynamic therapy can selectively kill tumor cells and reduce the harm to normal tissues. PDT is a non-surgical treatment of tumors using photodynamic reactions generated by photosensitizers and light. As a cold photochemical reaction, it mainly depends on the interaction between photosensitizer and light (commonly used laser), and is also affected by the oxygen concentration in the tissue. Therefore, photosensitizer is an important factor affecting the effect of PDT. [0003] T...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K49/18A61K9/50A61P35/00B82Y5/00B82Y15/00
CPCA61K9/5031A61K9/5078A61K41/0071A61K49/186A61P35/00B82Y5/00B82Y15/00
Inventor 文剑李清华陈志强
Owner AFFILIATED HOSPITAL OF GUILIN MEDICAL UNIV
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