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Lipidosome preparation, and preparation method and application thereof

A liposome preparation and liposome technology, applied in the field of biomedicine, can solve the problems of short timeliness, large injection dose, and limited efficiency of tumor hypoxia, and achieve the improvement of hypoxia, improvement of hypoxia, and improvement of hypoxia The effect of the situation

Active Publication Date: 2016-11-09
JE & NA BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the metformin that has been used to improve tumor hypoxia and improve radiotherapy is through intraperitoneal injection of metformin solution. This method usually has a short duration of action and a large injection dose, and its efficiency in improving tumor hypoxia is limited.

Method used

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  • Lipidosome preparation, and preparation method and application thereof
  • Lipidosome preparation, and preparation method and application thereof
  • Lipidosome preparation, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1, the preparation of liposome-metformin-chlorin e6 preparation

[0039] After weighing DPPC, DSPE-PEG, CH, and Ce6 according to the molar ratio of 6:0.5:4:1, they were dissolved in chloroform solution, and the chloroform solution was blown dry with nitrogen. Weigh metformin and dissolve it in phosphate buffer to prepare a 10 mg / mL metformin solution and add the prepared solution into liposome-chlorin e6, stir in a water bath at 65°C for 30 minutes, and place the prepared liposome -Metformin-chlorin e6 formulation extruded film.

Embodiment 2

[0040] Embodiment 2, the property detection of liposome-metformin-chlorin e6 preparation

[0041] The liposome-metformin-chlorin e6 preparation prepared in Example 1 was qualitatively detected, and transmission electron microscope detection, dynamic light scattering and ultraviolet absorption were respectively carried out.

[0042] Among them, the results of transmission electron microscope detection are as follows: figure 1 As shown, the results show that the liposome-metformin-chlorin e6 preparation prepared in Example 1 is distributed in a monodisperse state in water, indicating that the liposome-metformin-chlorin e6 preparation prepared by the present invention Uniform particle size, well dispersed in water.

[0043] The results of dynamic light scattering detection are as follows: figure 2 As shown, the results show that the particle size of the liposome-metformin-chlorin e6 preparation prepared in Example 1 is mainly concentrated at about 110nm in water.

[0044] The...

Embodiment 3

[0045] Embodiment 3, the photodynamic activity detection of liposome-metformin-chlorin e6 preparation

[0046] Prepare the aqueous solution of liposome-metformin-chlorin e6 preparation, and detect the singlet oxygen content through 661nm laser irradiation, and set up control groups respectively: Ce6 aqueous solution, liposome-metformin-chlorin e6 aqueous solution, water, Liposome-chlorin e6 aqueous solution, metformin aqueous solution. see results Figure 4 .

[0047] Figure 4 The results showed that the solutions containing the photosensitive molecule chlorin e6 all produced singlet oxygen after laser irradiation, and could be used as photodynamic therapy agents.

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Abstract

The invention belongs to the technical field of biological medicine, and relates to a lipidosome preparation, and a preparation method and application thereof, in particular to lipidosome-dimethyldiguanide-chlorin e6, and preparation and application thereof, particularly application to tumor photodynamic treatment and radiotherapy. The lipidosome preparation (lipidosome-dimethyldiguanide-chlorin e6) provided by the invention consists of a lipidosome, dimethyldiguanide and chlorin e6; the particle size is uniform; the dispersion in water is good; the particle size in water is mainly about 110nm; photosensitive molecules and the dimethyldiguanide can be simultaneously loaded; the lipidosome preparation is an ideal medicine carrier and has good stability. Experiments show that the lipidosome-dimethyldiguanide-chlorin e6 can be highly enriched in the tumor position; a continuous effect is achieved for lowering the tumor cell oxygen consumption quantity; the anoxia condition of the tumor position can be obviously improved; a good photodynamic treatment and radiotherapy effect is achieved.

Description

technical field [0001] The present invention relates to the technical field of biomedicine, relates to a liposome preparation and its preparation and application, in particular to liposome-metformin-chlorin e6 and its preparation and application, especially its application in tumor photodynamic therapy and radiotherapy in the application. Background technique [0002] Tumor refers to the neogrowth formed by the proliferation of local tissue cells in the body under the action of various tumorigenic factors, because this neogrowth is mostly in the form of space-occupying blocky protrusions, also known as neoplasm (neoplasm) . According to the cell characteristics of new organisms and the degree of harm to the body, tumors are divided into two categories: benign tumors and malignant tumors, and cancer is the general term for malignant tumors. Compared with benign tumors, malignant tumors grow faster and show invasive growth, are prone to bleeding, necrosis, ulcers, etc., and ...

Claims

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Application Information

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IPC IPC(8): A61K41/00A61K31/155A61K9/127A61P35/00
CPCA61K9/127A61K31/155A61K41/0071A61K2300/00
Inventor 刘庄宋雪娇
Owner JE & NA BIOTECH CO LTD
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