Pretreatment method for detecting organochlorine pesticide residue

A pesticide residue and organochlorine technology, applied in the field of pesticide science and testing, can solve the problems of vegetable export obstruction, human and animal poisoning, etc., and achieve the effects of less reagent volume, high precision and simple operation.

Inactive Publication Date: 2012-10-17
吴桂玲
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Every year in our country, incidents of human and animal poisoning caused by eating vegetables contaminated with

Method used

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  • Pretreatment method for detecting organochlorine pesticide residue
  • Pretreatment method for detecting organochlorine pesticide residue
  • Pretreatment method for detecting organochlorine pesticide residue

Examples

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Example Embodiment

[0026] Example 1 A pretreatment method for detecting organochlorine pesticide residues, including the following steps:

[0027] ⑴ After beating the fresh green vegetables according to the conventional method, the sample to be tested is obtained.

[0028] ⑵Add 40mL acetonitrile to 20g sample to be tested, homogenize at a rate of 8000r / min for 2min, then add 5g of dried NaCl to a 100mL centrifuge tube with stopper, and cover and shake vigorously for 2 min. Centrifuge at a speed of 4000 r / min for 2 minutes, and leave it at room temperature to obtain the supernatant; finally, draw 5 mL of the supernatant into a concentrating bottle at a rate of 180~280 r / min and a water bath temperature of 30~40℃ Under the conditions, use a rotary evaporator to evaporate to the remaining 1~2mL, and then use the ear ball to blow dry, and then the concentrate is obtained.

[0029] (3) Add 10 mL of eluent to the concentrate to completely dissolve the concentrate to obtain solution A, ready to be used; ...

Example Embodiment

[0034] Example 2 A pretreatment method for detecting organochlorine pesticide residues, including the following steps:

[0035] ⑴ After beating the fresh eggplant according to the conventional method, the sample to be tested is obtained.

[0036] (2) Add 50 mL of acetonitrile to 25 g of the sample to be tested, homogenize at a rate of 12000 r / min for 2 minutes, then add 6 g of dried NaCl to a 100 mL centrifuge tube with a stopper, and cover and shake vigorously for 3 minutes. Centrifuge at a speed of 4000 r / min for 3 min. After standing at room temperature, the supernatant is obtained; finally, 10 mL of the supernatant is drawn in a concentrated bottle at a rate of 180~280r / min and a water bath temperature of 30~40℃ Under the conditions of, use a rotary evaporator to evaporate to the remaining 1~2mL, and then blow dry with an ear ball to obtain the concentrate.

[0037] (3) Add 10 mL of eluent to the concentrate to completely dissolve the concentrate to obtain solution A, ready...

Example Embodiment

[0042] Example 3 A pretreatment method for detecting organochlorine pesticide residues, including the following steps:

[0043] ⑴ After beating the fresh tomatoes according to the conventional method, the sample to be tested is obtained.

[0044] (2) Add 60 mL of acetonitrile to 30 g of the sample to be tested, homogenize at a rate of 10000 r / min for 3 minutes, then add 8 g of dried NaCl to a 150 mL centrifuge tube with a stopper, and cover and shake vigorously for 3 minutes. Secondly, centrifuge at a speed of 5000 r / min for 4 minutes, and after standing at room temperature, the supernatant was obtained; finally, 15 mL of the supernatant was drawn in a concentrated bottle at a rate of 180~280r / min and a water bath temperature of 30~40 Under the condition of ℃, use a rotary evaporator to evaporate to the remaining 1~2mL, and then blow dry with an ear ball to obtain the concentrate.

[0045] (3) Add 15 mL of eluent to the concentrate to completely dissolve the concentrate to obta...

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Abstract

The invention relates to a pretreatment method for detecting organochlorine pesticide residue. The method comprises the steps of (1) pulping fresh vegetables and fruits to obtain a sample to be detected; (2) adding acetonitrile into the sample to be detected for blending, then adding dried NaCl, centrifuging, and standing at room temperature to obtain supernate, evaporating the supernate to obtain a concentrate; (3) adding leacheate into the concentrate, and completely dissolving to obtain a solution A; (4) prewashing an amino column or a graphitized carbon black-amino serial column by the leacheate, removing the leacheate, and pouring the solution A instantly; (5) conducting column chromatography on the solution A, then adding the leacheate, and collecting to obtain a solution B; (6) evaporating the elution solution B until the constant weight is achieved to obtain eluate, conducting constant volume on the eluate by normal hexane; and (7) filtering the eluate, adding the eluate into an automatic sample feeding bottle, and the detecting the eluate by a gas chromatography electron capture detector (ECD). According to the method, the simplicity and the rapidness are realized, the pesticide residues in the vegetables and the fruits are extracted, and the quantitative detection of the pesticide residues can be realized.

Description

technical field [0001] The invention relates to the technical field of pesticide science and detection, in particular to a pretreatment method for detecting organochlorine pesticide residues. Background technique [0002] Pesticide residues are "invisible killers" in vegetables. In recent years, with the improvement of people's living standards, the consumption of vegetables has increased year by year, and has become a potentially huge commercial market. In order to pursue economic interests, some vegetable farmers use a large amount of chemicals such as highly toxic and high residue pesticides, which has caused Excessive pesticide residues are common in vegetables. People are paying more and more attention to the problem of pesticide residues. Every year in our country, incidents of human and animal poisoning caused by eating vegetables contaminated by highly toxic pesticides occur, and even the export of vegetables is hindered due to excessive pesticide residues. Theref...

Claims

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Application Information

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IPC IPC(8): G01N30/12G01N30/02
Inventor 吴桂玲
Owner 吴桂玲
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