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Pretreatment kit for detecting Tilmicosin in animal muscle tissue and method thereof

A technology of tilmicosin and animal muscle, which is applied in the directions of measuring devices, instruments, scientific instruments, etc., to achieve the effects of good effect, low price and short analysis time

Inactive Publication Date: 2012-03-28
YANGTZE RIVER FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no reports of pretreatment kits for the detection of tilmicosin in animal muscle tissue at home and abroad

Method used

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  • Pretreatment kit for detecting Tilmicosin in animal muscle tissue and method thereof
  • Pretreatment kit for detecting Tilmicosin in animal muscle tissue and method thereof

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Experimental program
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Effect test

Embodiment 1

[0043] The present invention will be further described below in conjunction with the accompanying drawings.

[0044] A pretreatment kit for tilmicosin detection in animal muscle tissue, the pretreatment kit of the present invention is mainly composed of box body 1, reagent tube A2, reagent bag B3, reagent tube C4, reagent bottle D5, reagent bottle E6, reagent Bag F7. The box body is divided into 4 areas a, b, c, and d. Areas a and b are hole areas suitable for reagent tube A2 and reagent tube C4. Area c is for the grooves of reagent bag B3 and reagent bag F7. Area d is for Recesses for bottle D5 and bottle E6.

[0045] Preferably, the reagent tube A2 contains any liquid reagent in ethyl acetate, acetone, acetonitrile, methanol, and dichloromethane. The reagent A can be any liquid reagent in ethyl acetate, acetone, acetonitrile, methanol, and dichloromethane, and the amount of reagent A added is 5-10 mL.

[0046] Preferably, the reagent bag B3 contains any one of sodium chlo...

Embodiment 2

[0052] A sample processing method of a pretreatment kit for tilmicosin detection in animal muscle tissue, the steps are:

[0053] A. Take 2.0g of animal muscle tissue in a 50mL plastic centrifuge tube, add 5-10mL of reagent A, and shake for 30s; the reagent A can be any liquid reagent in ethyl acetate, acetone, acetonitrile, methanol, or methylene chloride .

[0054] B. Then add 2-10g of reagent B, shake for 30s, centrifuge at 5000r / min for 5min, transfer the supernatant to a heart bottle, and evaporate to dryness at 45°C; the reagent B can be sodium chloride, sodium carbonate, nitric acid Potassium, dipotassium hydrogen phosphate, potassium dihydrogen phosphate, ammonium dihydrogen phosphate, sodium sulfate or magnesium sulfate.

[0055] C. Add 2-10 mL of reagent C to the heart bottle, and transfer it to a 15 mL centrifuge tube; the reagent C can be any liquid reagent in ethyl acetate, acetone, acetonitrile, methanol, or methylene chloride.

[0056] D. Add 1-5mL of reagent ...

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Abstract

The invention discloses a pretreatment kit for detecting Tilmicosin in an animal muscle tissue and a method thereof. The kit comprises a box body, a reagent tube A, a reagent bag B, a reagent tube C, a reagent bottle D, a reagent bottle E, and a reagent bag F, wherein the inside of the box body is divided into a zone a, a zone b, a zone c and a zone d, the zone a and the zone b are aperture zonesfor placing the reagent tube A and the reagent tube C, the zone c is a groove zone for placing the reagent bag B and the reagent bag F, and the zone d is a groove zone for placing the reagent bottle D and the reagent bottle E. The method comprises the following steps: 1, placing the animal muscle tissue in a centrifuge tube, adding a reagent to the centrifuge tube, and oscillating; 2, adding a reagent, and carrying out rotary evaporation to dryness; 3, adding a reagent to an inverted conical bottle, and transferring the obtained solution to the centrifuge tube; 4, adding a reagent solution, oscillating, and drying through blowing by a pressured gas blowing concentrator; and 5, dissolving the residue by a mixed liquid reagent solution, adding n-hexane, carrying out vortex oscillation, removing the upper liquid, filtering the lower liquid by a filtering head, and determining by UPLC (ultrahigh performance liquid chromatography). The method of the invention has the advantages of simple operation, rapidness, low price, and realization of no damage of the reagents to the chromatographic column.

Description

technical field [0001] The invention belongs to the technical field of food safety analysis, and more specifically relates to a pretreatment kit for detecting tilmicisin (TLM) in animal muscle tissue, and also relates to a detection of tilmicisin residue in animal muscle tissue The method, this kit and detection method are suitable for sample pretreatment and detection of tilmicosin residue detection in muscle tissue of fresh live animals (fish, shrimp, pig, chicken). Background technique [0002] Tilmicosin was successfully developed by the British Elanco Animal Health Products Company in the 1980s. It is mainly used for infectious diseases caused by sensitive bacteria in cattle, dairy cows, goats, sheep, pigs, chickens, fish and other animals. Tilmicosin is a semi-synthetic macrolide antibiotic, a derivative of Tylosin, which has a similar antibacterial spectrum as Tylosin, and is effective against Gram-positive bacteria and some Gram-positive bacteria. Negative bacteria,...

Claims

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Application Information

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IPC IPC(8): G01N30/14
Inventor 刘永涛艾晓辉邹世平何力杨红喻亚丽
Owner YANGTZE RIVER FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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