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Preparation method for chemical reference substance of sweroside in Tibetan capillary artemisia medicinal material

A technology of alginol chemistry and serrata, applied in chemical instruments and methods, organic chemistry, preparation of sugar derivatives, etc., can solve the problem that there is no public patent for the preparation method of sagoside, and achieves easy popularization and stability. Good performance and the effect of increasing production

Inactive Publication Date: 2013-11-27
CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The currently reported preparation method of swertiol glycoside mainly adopts column chromatography, and there is no published patent on the preparation method of swertiol glycoside

Method used

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  • Preparation method for chemical reference substance of sweroside in Tibetan capillary artemisia medicinal material
  • Preparation method for chemical reference substance of sweroside in Tibetan capillary artemisia medicinal material
  • Preparation method for chemical reference substance of sweroside in Tibetan capillary artemisia medicinal material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 The preparation method of the chemical reference substance of swertiol glycosides in the Tibetan capillary chinensis medicinal material comprises the following steps:

[0035] ⑴Preparation of extract extract:

[0036] The Tibetan Yinchen medicinal material—Swertia chinensis in western Sichuan was extracted by heating and refluxing with 95% ethanol at a ratio of 1 g:5 mL at an extraction temperature of 60°C, extraction times were 4 times, and each time was 1 h, and combined extraction liquid; the extract was concentrated under reduced pressure at a pressure of 0.02 MPa to obtain the extract of Capillary wormwood extract.

[0037] ⑵Extraction:

[0038] After dissolving the extract extract of Yinchen chanterelles with 1 times its mass in deionized water, it was extracted with chloroform and n-butanol in sequence to obtain the chloroform fraction, n-butanol fraction and water fraction respectively.

[0039] (3) The n-butanol fraction is freeze-dried to a c...

Embodiment 2

[0047] Example 2 The preparation method of the chemical reference substance of swertiol glycosides in the Tibetan capillary chinensis medicinal material comprises the following steps:

[0048] ⑴Preparation of extract extract:

[0049]The Tibetan Yinchen medicinal material——Chuanxi Swertia was extracted by heating and reflux at the ratio of 1 g: 30 mL with 5% ethanol at the extraction temperature of 95°C, twice for 4 h each time, and combined Extract solution; the extract solution is concentrated under reduced pressure at a pressure of 0.07 MPa to obtain the extract of Capillary cypress extract.

[0050] ⑵Extraction:

[0051] After dissolving the extract of Capillary root extract with 10 times its mass in deionized water, it was extracted with chloroform and n-butanol in sequence to obtain the chloroform fraction, n-butanol fraction and water fraction respectively.

[0052] (3) For the n-butanol part, the feeding flow rate is 200mL / h, the feeding temperature is 30°C, th...

Embodiment 3

[0060] Example 3 The preparation method of the chemical reference substance of swertiol glycosides in the Tibetan capillary chinensis medicinal material comprises the following steps:

[0061] ⑴Preparation of extract extract:

[0062] The Tibetan Yinchen medicinal material—Swertia chinensis in western Sichuan was extracted by heating and reflux at a ratio of 1 g:10 mL with 20% ethanol at an extraction temperature of 80°C, three times for 3 h each time, and combined Extract solution; the extract solution is concentrated under reduced pressure at a pressure of 0.05 MPa to obtain the extract of Capillary wormwood extract.

[0063] ⑵Extraction:

[0064] After dissolving the extract extract of Yinchen chanterelles with 5 times its mass in deionized water, it was extracted with chloroform and n-butanol in sequence to obtain the chloroform part, n-butanol part and water part respectively.

[0065] (3) The n-butanol fraction is oven-dried to constant weight at a temperature of...

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Abstract

The invention relates to a preparation method for a chemical reference substance of sweroside in a Tibetan capillary artemisia medicinal material. The method comprises steps of (1) subjecting the Tibetan capillary artemisia medicinal material to alcohol extraction, and concentration under reduced pressure to obtain a Tibetan capillary artemisia extract; (2) extracting with chloroform and n-butanol separately, after the Tibetan capillary artemisia extract is dissolved, to obtain a chloroform part, an n-butanol part and a water part; (3) drying the n-butanol part to constant weight to obtain dry powder of the n-butanol part; (4) dissolving the dry powder of the n-butanol part by using n-butanol and injecting into a preparative high-performance liquid chromatography, collecting an eluate A after elution and on-line ultraviolet detection, and drying the eluate A to obtain a sweroside-rich fraction with a purity larger than 80%; and (5) dissolving the sweroside-rich fraction, adding into a gel separation column to elute to obtain an eluate B, and drying the eluate B to obtain a sweroside-rich fraction with a purity larger than 98%, thus obtaining the chemical reference substance of the sweroside. The preparation method has simple technology, high repeatability and high recovery rate.

Description

technical field [0001] The invention relates to a preparation method of a chemical reference substance, in particular to a preparation method of a chemical reference substance of swertiol glycosides in Tibetan capillary chinensis medicinal materials. Background technique [0002] Tibetan Yinchen is a famous Tibetan medicinal material for the treatment of liver and gallbladder diseases such as hepatitis, cholecystitis, gallbladder and jaundice. Its main active ingredients are iridoids and ketones. Exciting and inhibiting, diuretic, cardiotonic and other activities and detoxification, liver protection, anti-inflammatory, anti-viral and other activities. 7-O-[α-L-rhamnopyranose-(1→2)-β-D-xylopyranose]-1,8-dihydroxy-3-methoxybutanone (7-O -[α-L-rhamnopyranosyl-(1→2)-β-D-xylopyranosyl]-1,8-dihydroxy-3-methoxyxanthone) or named Swertioside, is a kind of medicinal material Koushan ketone glycosides are one of the active ingredients of Zang Yinchen for treating hepatitis. Its chem...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/04C07H1/08
Inventor 陈晨陈涛李玉林孙菁
Owner CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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