Quality control method of lycium ruthenicum pigment

A quality control method and technology of Lycium rutabaga, applied in the measurement of color/spectral characteristics, analysis by chemical reaction of the material, material analysis by observing the effect on chemical indicators, etc. quality control methods, unfavorable black fruit Lycium barbarum pigment effectiveness and other issues, to achieve the effect of easy promotion, high sensitivity, and less reagents

Inactive Publication Date: 2010-10-13
CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, there is currently no complete quality control method for the black fruit wolfberry pigme

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1 A method for quality control of Lycium barbarum pigment, the method uses the determination of total anthocyanin content, proanthocyanidin content and total polyphenol content as quality control indicators. in

[0025] ——The determination method of total anthocyanin content is:

[0026] (1) Preparation of sample solution: Take 0.1g of Lycium barbarum pigment, put it in a 50ml measuring bottle, add water to dissolve, set the volume to the mark, and shake well. Precisely measure 2ml, put it in a 10ml measuring bottle, dilute with water to the mark, and shake well; take 1ml into a test tube, add 10mL of 0.1% hydrochloric acid-80% ethanol solution, and keep it warm at 25°C for 24 hours.

[0027] (2) Determination of the absorbance value of the sample solution: the absorbance value of the sample solution was measured at a wavelength of 540 nm by an ultraviolet-visible spectrophotometer to be 0.47.

[0028] (3) Calculate the total anthocyanin content in the samp...

Embodiment 2

[0046] Embodiment 2 A method for quality control of Lycium barbarum pigment, which uses the determination of total anthocyanin content, proanthocyanidin content and total polyphenol content as quality control indicators. in

[0047] ——The determination method of total anthocyanin content is:

[0048] (1) Preparation of sample solution: Take 0.05g of Lycium barbarum pigment, put it in a 10ml measuring bottle, add water to dissolve, set the volume to the mark, and shake well. Precisely measure 5ml, put it in a 10ml measuring bottle, dilute to the mark with water, and shake well; take 2ml into a test tube, add 20mL of 0.01% hydrochloric acid-50% ethanol solution, and keep warm at 15°C for 36 hours to obtain.

[0049] (2) Determination of the absorbance value of the sample solution: measure the absorbance value of the sample solution at a wavelength of 450 nm with an ultraviolet-visible spectrophotometer to be 2.5.

[0050] (3) Calculate the total anthocyanin content in the samp...

Embodiment 3

[0063] Embodiment 3 is a method for quality control of Lycium barbarum pigment, which uses the determination of total anthocyanin content, proanthocyanidin content and total polyphenol content as quality control indicators. in

[0064] ——The determination method of total anthocyanin content is:

[0065] (1) Preparation of sample solution: Take 5g of Lycium barbarum pigment, put it in a 100ml measuring bottle, add water to dissolve, set the volume to the mark, and shake well. Accurately measure 2ml, put it in a 100ml measuring bottle, dilute to the mark with water, and shake well; take 10ml into a test tube, add 25mL of 1% hydrochloric acid-90% ethanol solution, and keep warm at 80°C for 1 hour to obtain.

[0066] (2) Determination of the absorbance value of the sample solution: measure the absorbance value of the sample solution at a wavelength of 600 nm with an ultraviolet-visible spectrophotometer to be 1.1.

[0067] (3) Calculate the total anthocyanin content in the sampl...

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Abstract

The invention relates to a quality control method of lycium ruthenicum pigment. In the method, determination of a total anthocyanin content, a proanthocyanidins content and a total polyphenols content is taken as a quality control index, and the method has the advantages of little dosage of used reagent, simple and convenient operation, high flexibility, high precision, favorable stability and the like and can realize effective evaluation of biological activity on a lycium ruthenicum pigment.

Description

technical field [0001] The invention relates to a method for controlling the quality of a pigment, in particular to a method for controlling the quality of a black wolfberry pigment. Background technique [0002] Black fruit wolfberry pigment is a pigment extracted from the mature fruit of Lycium ruthenicum Murr., a plant of the genus Lycium ruthenicum Murr. in the family Solanaceae. It has strong antioxidant properties and belongs to natural anthocyanin plant pigments. It is purple red and powdery. Non-toxic, used in medicine, food and cosmetics. [0003] The pigments of Lycium barbarum mainly contain anthocyanins, proanthocyanidins and total polyphenols. Among them, anthocyanins are one of the water-soluble plant pigments, widely present in the flowers and fruits of plants, belonging to flavonoids in phenolic compounds, which have the functions of scavenging free radicals in the body, anti-oxidation, lowering blood fat and preventing atherosclerosis, etc. Function; Proan...

Claims

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Application Information

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IPC IPC(8): G01N21/33G01N21/78
Inventor 邵赟陶燕铎陈晨文怀秀赵晓辉梅丽娟张兴旺
Owner CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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