Method for detecting antioxidant constituents in nitraria fruit juice by using 1,1-diphenyl-2-picrylhydrazyl (DPPH)-high performance liquid chromatography (HPLC) method

An anti-oxidant component and anti-oxidant technology, applied in the direction of measuring devices, material separation, and analysis of materials, can solve problems such as unfavorable quality of Nitraria japonica juice, and achieve the effect of easy promotion, less reagent amount, and good stability

Inactive Publication Date: 2013-01-16
CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, there is no complete detection method for the antioxidant components of Nitraria juice, which is not conducive to ensuring the quality of Nitraria juice

Method used

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  • Method for detecting antioxidant constituents in nitraria fruit juice by using 1,1-diphenyl-2-picrylhydrazyl (DPPH)-high performance liquid chromatography (HPLC) method
  • Method for detecting antioxidant constituents in nitraria fruit juice by using 1,1-diphenyl-2-picrylhydrazyl (DPPH)-high performance liquid chromatography (HPLC) method

Examples

Experimental program
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Embodiment 1

[0014] Example 1 A DPPH-HPLC method for detecting antioxidant components in Nitraria japonica juice, the method first mixes 0.01% antioxidant 1,1-diphenyl-2-trinitrophenylhydrazine (DPPH) with 5000 Dal The white thorn fruit juice filtered by the organic membrane was reacted in the dark at a temperature of 35°C for 15 minutes at a volume ratio of 100:1 (mL / mL) to obtain the reacted white thorn juice; then the unreacted white thorn juice was mixed with After the reaction, the Nitraria juice was injected into high-performance liquid chromatography (HPLC) for detection, and the chromatograms of the unreacted Nitraria juice and the chromatogram of the reacted Nitraria juice were obtained respectively; finally, the chromatograms of the unreacted Nitraria juice and the reaction The peak area of ​​each corresponding peak in the chromatogram of Nitraria juice; if the peak area decreases, it means that there are antioxidant components in Nitraria juice.

[0015] Among them: the dete...

Embodiment 2

[0016] Example 2 A DPPH-HPLC method for detecting antioxidant components in Nitraria fruit juice, the method firstly mixes the antioxidant 1,1-diphenyl-2-trinitrophenylhydrazine (DPPH) with a mass concentration of 5% and Nitraria The original fruit juice was reacted in the dark at a temperature of 25°C for 30 minutes at a volume ratio of 150:1 to obtain the reacted Nitraria juice; then the unreacted Nitraria juice and the reacted Nitraria juice were injected into high performance liquid chromatography (HPLC). ) to obtain the chromatogram of the unreacted Nitraria juice and the chromatogram of the reacted Nitraria juice; finally, compare the chromatogram of the unreacted Nitraria juice with the corresponding peaks in the chromatogram of the reacted Nitraria juice Peak area; if the peak area decreases, it means that there are antioxidant components in Nitraria japonica juice.

[0017] Among them: the detection method of high performance liquid chromatography (HPLC) refers to...

Embodiment 3

[0018] Example 3 A DPPH-HPLC method for detecting antioxidant components in Nitraria japonica fruit juice, the method first mixes 0.1% antioxidant 1,1-diphenyl-2-trinitrophenylhydrazine (DPPH) with 5000 Dal The Nitraria juice filtered by the organic membrane was reacted in the dark for 20 minutes at a temperature of 30°C at a volume ratio of 200:1 (mL / mL) to obtain the reacted Nitraria juice; then the unreacted Nitraria juice was mixed with After the reaction, the Nitraria juice was injected into high-performance liquid chromatography (HPLC) for detection, and the chromatograms of the unreacted Nitraria juice and the chromatogram of the reacted Nitraria juice were obtained respectively; finally, the chromatograms of the unreacted Nitraria juice and the reaction The peak area of ​​each corresponding peak in the chromatogram of Nitraria juice; if the peak area decreases, it means that there are antioxidant components in Nitraria juice.

[0019] Among them: the detection meth...

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Abstract

The invention relates to a method for detecting antioxidant constituents in nitraria fruit juice by using a DPPH-HPLC method. The method comprises that firstly an antioxidant 1,1-diphenyl-2-trinitrobenzene hydrazine and the nitraria fruit juice are subjected to photophobic reaction to obtain nitraria fruit juice after reaction; then nitraria fruit juice which is not subjected to reaction and the nitraria fruit juice after reaction are respectively injected into the high performance liquid chromatography for detection, and a chromatogram of the nitraria fruit juice which is not subjected to reaction and a chromatogram of the nitraria fruit juice after reaction are respectively ontained; and finally, peak areas of corresponding peaks in the chromatogram of the nitraria fruit juice which is not subjected to reaction and the chromatogram of the nitraria fruit juice after reaction are compared; and if the peak areas of the peaks are reduced, antioxidant constituents exist in the nitraria fruit juice. According to the method, the amount of the used reagent is low, the operation is convenient and simple, the sensitivity is high, the accuracy is high, the stability is good, and effective evaluation for the antioxidant constituents in nitraria fruit juice can be achieved.

Description

technical field [0001] The invention relates to a method for detecting antioxidant components in fruit juice, in particular to a method for detecting antioxidant components in Nitraria japonicus juice by DPPH-HPLC. Background technique [0002] Nitraria juice is based on the Nitraria plant Nitraria ( Nitraria sibirica ) The fruit juice obtained after removing the seed skin from the mature fruit has strong antioxidant properties because it contains antioxidant substances. It is purple in color and non-toxic. It is used in medicine, food and cosmetics. However, at present, there is no complete detection method for the antioxidant components of Nitraria juice, which is not conducive to ensuring the quality of Nitraria juice. Contents of the invention [0003] The technical problem to be solved by the present invention is to provide a simple, fast and accurate DPPH-HPLC method for detecting antioxidant components in Nitraria japonica juice. [0004] In order to solve the abo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 陈晨李玉林陶燕铎邵赟梅丽娟赵晓辉岳会兰陈涛
Owner CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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