Method for rapid determination of selenium content in tetrastigmatis hemsleyani

A rapid determination and clover technology, applied in the field of physical and chemical testing, can solve the problems of cumbersome wet digestion procedures, long digestion cycle, long measurement cycle, etc., and achieve the effect of shortening the detection cycle, weak interference, and fast detection

Inactive Publication Date: 2014-10-29
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Such as "Determination of Selenium in Feedstuff" (GB / T13883-2008), "Detection Method of Selenium Content in Tea Leaves" (GB / T21729-2008), "National Food Safety Standard for Determination of Selenium in Food (GB5009.93-2010) etc., the above methods all use wet digestion, which is complicated to operate; when microwave digestion is used, acid treatment is required, and the digestion cycle is long; and in the measurement process, 6mol / L hydrochloric acid solution and potassium ferricyanide solution are used, reagents Large dosage, complex operation
[0007] The disclosed methods for measuring selenium content by hydride generation-atomic fluorescence spectrometry include: Chinese patent applications with patent application numbers 20111027682.0, 201110427886.4 and 201110427880.7 respectively disclose methods for rapidly measuring selenium content in plant samples, animal tissues and soil, Both use nitric acid-perchloric acid mixed acid wet digestion, hydride generation-atomic fluorescence spectrometry determination, but the wet digestion procedure is cumbersome, the operation is complicated, and the error is large
[0008] The existing methods have disadvantages such as complex operation or long measurement period

Method used

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  • Method for rapid determination of selenium content in tetrastigmatis hemsleyani
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  • Method for rapid determination of selenium content in tetrastigmatis hemsleyani

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] (1) Experimental instrument: the atomic fluorescence spectrometer used in this example is AFS-8220 from Beijing Jitian Instrument Co., Ltd.

[0030] (2) Sample pretreatment: Wash the Clover sample, dry it at 50°C, pulverize it with a sample mill, pass through a 24-mesh sieve, store it in a ziplock bag, and stick a label to obtain the sample to be tested for future use.

[0031] (3) Reagents and preparation:

[0032] Nitric acid (excellent pure);

[0033] Hydrochloric acid (excellent pure);

[0034] Potassium hydroxide (excellent pure);

[0035] Reductant: Potassium borohydride, dissolving an appropriate amount of potassium borohydride in a potassium hydroxide solution with a mass concentration of 5g / L to prepare a potassium borohydride solution with a mass concentration of 8g / L, set to volume, shake well, and set aside;

[0036] Carrier current: hydrochloric acid solution with a volume concentration of 5%

[0037] Single element selenium standard solution: 1000mg / L;...

Embodiment 2

[0050] (1) Experimental instrument: the atomic fluorescence spectrometer used in this example is AFS-8220 from Beijing Jitian Instrument Co., Ltd.

[0051] (2) Sample pretreatment: Wash the Clover sample, dry it at 50°C, pulverize it with a sample mill, pass through a 24-mesh sieve, store it in a ziplock bag, and stick a label to obtain the sample to be tested for future use.

[0052] (3) Reagents and preparation:

[0053] Nitric acid (excellent pure);

[0054] Hydrochloric acid (excellent pure);

[0055] Potassium hydroxide (excellent pure);

[0056] Reductant: Potassium borohydride, dissolving an appropriate amount of potassium borohydride in a potassium hydroxide solution with a mass concentration of 5g / L to prepare a potassium borohydride solution with a mass concentration of 8g / L, set to volume, shake well, and set aside;

[0057] Carrier current: hydrochloric acid solution with a volume concentration of 5%

[0058] Single element selenium standard solution: 1000mg / L;...

Embodiment 3

[0071] (1) Experimental instrument: the atomic fluorescence spectrometer used in this example is AFS-8220 from Beijing Jitian Instrument Co., Ltd.

[0072] (2) Sample pretreatment: Wash the Clover sample, dry it at 55°C, pulverize it with a sample mill, pass through a 24-mesh sieve, store it in a ziplock bag, and stick a label to obtain the sample to be tested for future use.

[0073] (3) Reagents and preparation:

[0074] Nitric acid (excellent pure);

[0075] Hydrochloric acid (excellent pure);

[0076] Potassium hydroxide (excellent pure);

[0077] Reductant: Potassium borohydride, dissolving an appropriate amount of potassium borohydride in a potassium hydroxide solution with a mass concentration of 5g / L to prepare a potassium borohydride solution with a mass concentration of 8g / L, set to volume, shake well, and set aside;

[0078] Carrier current: hydrochloric acid solution with a volume concentration of 5%

[0079] Single element selenium standard solution: 1000mg / L;...

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Abstract

The invention discloses a method for rapid determination of the selenium content in tetrastigmatis hemsleyani, wherein a microwave digestion-hydride generation-atomic fluorescence spectrometry method is adopted for determination of the selenium content. With microwave digestion, the digestion efficiency is high and fast, moreover, acid removing is not required after digestion, operation steps are simplified, pollution is reduced, the accuracy rate is increased, disadvantages of easy pollution of wet digestion and long digestion time are avoided, acid removing is not required, concentrated hydrochloric acid is directly added for reduction of selenium, and the digestion procedures are simplified; the detection cycle is greatly shortened, and the original detection cycle of 8-10 hours is shortened to 2-3 hours; and the detection is rapid and accurate, and the operation is simple.

Description

technical field [0001] The invention belongs to the field of physical and chemical testing, in particular to a method for quickly measuring the selenium content in Pseudomonas clover. Background technique [0002] Clover (Tetrastigmatis hemsleyani), also known as: horned black berry, stone mouse, is a plant of the genus Cliffaceae of the grape family, and its tuberous root is called snake aconite. Clover is mainly distributed in the south of the Yangtze River. It is born in damp hillsides, ravines or forests next to valleys. It is a Chinese herbal medicine commonly used in Guangxi folks with tubers or whole herbs. It has antiviral, anti-inflammatory, analgesic and anti-inflammatory properties. Antipyretic, liver protection and other effects, and side effects are small, it is a "plant antibiotic" that cannot be replaced by western medicine. In recent years, studies have found that trifoliate green ethyl acetate extract is the most effective component of commonly used anti-tu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
Inventor 谢景千付金娥韦树根龙海荣谷筱玉
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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