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Antimicrobial compound and preparation method and application thereof

A compound and chemical technology, applied in the field of microorganisms, can solve the problems of narrow bactericidal spectrum and low toxicity of fish, and achieve the effect of improving utilization rate, increasing concentration and increasing yield

Inactive Publication Date: 2011-02-09
CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has a narrow bactericidal spectrum, is very safe for humans and animals, and has low toxicity to fish

Method used

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  • Antimicrobial compound and preparation method and application thereof
  • Antimicrobial compound and preparation method and application thereof
  • Antimicrobial compound and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Use 10 1000mL Erlenmeyer flasks, each containing 300mL medium A (glucose 2.0%, peptone 0.8%, corn flour 4.0%, soybean meal 3.0%, yeast powder 0.5%, magnesium sulfate 0.05%, potassium dihydrogen phosphate 0.5%) , sterilized at 120° C. for 30 minutes, cooled and inoculated with activated Streptomyces noursii XiAo-3 spore liquid, placed at 28° C., and shaken for 36 hours. Inoculate the cultured strain solution into a 100L fermenter with 50L sterilized medium B in a 10% inoculum size, (substrate B: 1.0% glycerol, 7.0% sucrose, 0.1% ammonium sulfate, 8.0% glucose, Peptone 2.0%, soybean flour or soybean flour hydrolyzate 5.0%, peanut flour or peanut flour hydrolysis 6.0%, biological nitrogen 6.0%, corn flour 3.0%, yeast powder 1.0%, dipotassium hydrogen phosphate 1.0%, magnesium sulfate 0.5% , sodium chloride 0.5%, calcium chloride 0.5%). At a temperature of 28°C-30°C, after 14 hours of aeration and stirring fermentation, medium C was continuously fed at a rate of 0.5L / h (me...

Embodiment 2

[0069]Use 12 1000mL Erlenmeyer flasks, each containing 300mL medium A (glucose 3.0%, peptone 1.0%, corn flour 1.0%, soybean meal 2.0%, yeast powder 0.5%, magnesium sulfate 0.5%, potassium dihydrogen phosphate 0.2%) , sterilized at 120° C. for 30 minutes, cooled and inoculated with activated Streptomyces noursii XiAo-3 spore liquid, placed at 28° C., and shaken for 40 hours. Inoculate the cultured strain solution into a 100L fermenter with 50L sterilized medium B inside at an inoculum size of 15%, (medium B: glycerol 1.0%, sucrose / molasses 2.0%, ammonium sulfate 0.5%, glucose 10.0 %, peptone 4.0%, soybean flour or soybean flour hydrolyzate 5.0%, peanut flour or peanut flour hydrolyzate 5.0%, biological nitrogen 5.0%, corn flour 3.0%, yeast powder 1.0%, dipotassium hydrogen phosphate 2.5%, sulfuric acid Magnesium 1.5%, Sodium Chloride 1.5%, Calcium Chloride 2.0%). At a temperature of 28°C-30°C, after 18 hours of aeration and stirring fermentation, medium C (medium C: 1.0% sucro...

Embodiment 3

[0074] Use 10 1000mL Erlenmeyer flasks, each containing 300mL medium A (glucose 3.0%, peptone 1.0%, corn flour 1.0%, soybean meal 2.0%, yeast powder 0.5%, magnesium sulfate 0.5%, potassium dihydrogen phosphate 0.2%) , sterilized at 120° C. for 30 minutes, cooled and inoculated with activated Streptomyces noursii XiAo-3 spore liquid, placed at 28° C., and shaken for 36 hours. Inoculate the cultured strain solution into a 100L fermenter with 50L sterile medium B inside at an inoculum size of 20%, (medium B: glycerol 1.0%, sucrose / molasses 2.0%, ammonium sulfate 1.0%, glucose 10.0 %, peptone 3.0%, soybean flour or soybean flour hydrolyzate 5.0%, peanut flour or peanut flour hydrolyzate 6.0%, biological nitrogen 4.0%, corn flour 3.0%, yeast powder 2.0%, dipotassium hydrogen phosphate 1.0%, sulfuric acid Magnesium 1.0%, Sodium Chloride 1.0%, Calcium Chloride 1.0%). At a temperature of 28°C-30°C, after 25 hours of aeration and stirring fermentation, medium C was added intermittentl...

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Abstract

The invention belongs to the technical field of microbes, and in particular relates to an antimicrobial compound and a fermenting preparation method and application thereof. The molecular weight of the antimicrobial compound of the invention is 445, the molecular formula of the antimicrobial compound is C16H23N5O10, the chemical name of the antimicrobial compound is 1-uracil-4-sarkosyl-serylamido-1,4-dideoxy-beta-D-glucopyranosyl acid, and the antimicrobial compound belongs to uracil hexose dipeptide and is named xin'aomycin. The preparation method adopts actinomycetes Streptomyces noursei and genetically improved strains thereof, and comprises the following steps: culturing in a primary liquid culture medium as a seed liquid; inoculating the cultured seed liquid to a secondary liquid culture medium B for culturing, and carrying out fed-batch fermentation culture of fed-batch liquid C; and after fermentation is finished, separating and purifying the structural compound from the fermentation culture liquid. The antimicrobial compound is used for manufacturing pesticide or medicine products. The invention has the characteristics of high yield, simple process and easy industrialization.

Description

[0001] Field [0002] The invention belongs to the technical field of microorganisms, and in particular relates to an antibacterial compound with a new structure, its fermentation preparation method and application. Background technique [0003] Microbial metabolites are the main source of antibiotics, and actinomycetes are one of the most important types of microorganisms that produce antibiotics. Since the discovery of streptomycin, a large number of antibiotics have been discovered from actinomycetes. In recent years, people have searched for new antibiotics from Streptomyces vulgaris, Actinomycetes in extreme environments, Actinomycetes marine and rare Actinomycetes. For example, gentamicin, erythromycin, vancomycin, rifampicin, etc. have been successfully applied clinically; antibiotics used in the control of crop diseases and insect pests include glutaricin, blasticidin, antimycin 120, wuyiencin, etc. . [0004] Blasticidin is an antibiotic produced by soil actinomyce...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12R1/57A01N63/02C12P21/02C07K5/062A61K38/05A61P31/12A01P1/00A61P31/04
Inventor 周金燕谭红钟娟李志东杨杰肖亮刘东
Owner CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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