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Thermostable reverse transcriptase

A technology of reverse transcriptase and hydrothermal solution, which is applied to the determination/inspection of transferases, enzymes, microorganisms, etc., and can solve problems such as unsatisfactory thermal stability, demand, and short half-life

Pending Publication Date: 2021-03-05
GUANGDONG FAPON BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] There are many studies in the prior art through the transformation of reverse transcriptase. After transformation, although the reaction temperature of reverse transcriptase can be increased, its half-life is still relatively short, and its thermal stability still cannot meet the requirements in some cases. field needs

Method used

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Examples

Experimental program
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Effect test

Embodiment

[0066] 1. Fusion protein

[0067] Taking the following DNA-binding proteins derived from extremophiles or extremophile metagenomes above OTG75°C as examples, MMLV was used as an example to construct:

[0068] The amino acid sequence of the DNA-binding protein Sso7d derived from Saccharolobus solfataricus is shown in SEQID NO:1;

[0069] The amino acid sequence of the DNA binding protein Pfud derived from Pyrococcus furiosus is shown in SEQ ID NO:2;

[0070] The amino acid sequence of the DNA-binding protein Tlid derived from Thermococcus litoralis is shown in SEQ ID NO: 3;

[0071] The amino acid sequence of the DNA binding protein Ventd derived from the hydrothermal vent metagenome is shown in SEQ ID NO:4.

[0072] 2. Fusion of reverse transcriptase and DNA binding protein

[0073]Fusion of Sso7d at the C-terminus of the MMLV mutant (the amino acid sequence of MMLV is shown in SEQ ID NO: 5, the mutation site L435K / D524N, named KNMMLV after mutation) to obtain KNMMLV-Sso7d ...

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Abstract

The invention relates to the technical field of biology, in particular to a thermostable reverse transcriptase to which a DNA binding protein derived from a high-temperature-resistant eosinophile is connected, the thermostability of the reverse transcriptase can be improved, and higher continuous synthesis capacity and template binding capacity are obtained.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a thermostable reverse transcriptase. Background technique [0002] Reverse transcriptase is a general term for DNA polymerases that rely on RNA as a template. American scientists H.M.Temin and D.Baltimore discovered reverse transcriptase in 1970, and won the Nobel Prize in Physiology and Medicine in 1975 for this. The discovery of reverse transcriptase has greatly promoted genetic engineering technology. It is an indispensable tool for studying eukaryotic or prokaryotic target genes and constructing cDNA libraries. Together with Taq enzymes, it constitutes the basic tool of modern biotechnology. enzyme. Currently commercialized reverse transcriptases such as MMLV (molomey murine leukemia virus) have limited amplification capabilities, and the length of cDNA fragments generally obtained is not more than 6kb, which is not enough for the construction of cDNA fragments. At the same ti...

Claims

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Application Information

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IPC IPC(8): C12N9/12C12N15/54C12Q1/6869
CPCC12N9/1276C12Q1/6869C12Y207/07049C07K2319/00C12Q2521/107C12Q2527/125C12Q2535/122
Inventor 钟淑瑶章瑞程杜翔斐黄杏帆卢庆庆
Owner GUANGDONG FAPON BIOTECH CO LTD
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