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Method for producing glucosamine through fermentation of escherichia coli

A technology of glucosamine and Escherichia coli, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problems of low production of glucosamine and difficult high-efficiency production, and achieve high-efficiency fermentation production and industrialized production , cost reduction effect

Inactive Publication Date: 2019-12-17
SHANDONG RUNDE BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the prior art, the ammonia sugar production capacity of this process is low, and it is difficult to realize industrialized efficient production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Activate and culture Escherichia coli, Bacillus subtilis, and Staphylococcus on a plate medium for 6 hours, control the culture temperature at 37°C, and shake the table at a speed of 200 rpm; mix the cultured Escherichia coli, Saccharomyces cerevisiae, and Staphylococcus in a volume ratio of 3:1:0.8 After inoculation, activate the culture in the seed medium, control the culture temperature at 37°C, shaker speed at 230rpm, culture time for 12h, adjust the pH value to 6.9 with ammonia water; In the fermentation medium, the fermentation culture is carried out in a fermenter, the culture temperature is 37°C, the shaker speed is 230rpm, the culture time is 40h, the pH value is adjusted to 6.9 with ammonia water, and the fermentation liquid containing glucosamine is obtained, and the glucose feeding is controlled during the whole fermentation process. The speed is 4L / h, and the glucosamine content in the detected fermentation broth is 82.4g / L.

Embodiment 2

[0025] Activate and culture Escherichia coli, Bacillus subtilis, and Staphylococcus on a plate medium for 6 hours, control the culture temperature at 37°C, and shake the table at a speed of 200 rpm; mix the cultured Escherichia coli, Saccharomyces cerevisiae, and Staphylococcus in a volume ratio of 3:1.2:0.8 After inoculation, activate the culture in the seed medium, control the culture temperature at 36°C, shaker speed at 240rpm, culture time for 13h, adjust the pH value to 6.8 with ammonia water; In the fermentation medium, the fermentation culture is carried out in a fermenter, the culture temperature is 36°C, the shaker speed is 240rpm, the culture time is 38h, the pH value is adjusted to 6.8 with ammonia water, and the fermentation liquid containing glucosamine is obtained, and the glucose feeding is controlled during the whole fermentation process. The speed is 4.5L / h, and the glucosamine content in the detected fermentation broth is 80.7g / L.

Embodiment 3

[0027] Activate and culture Escherichia coli, Bacillus subtilis, and Staphylococcus on a plate medium for 6 hours, control the culture temperature at 37°C, and shake the table at a speed of 200 rpm; mix the cultured Escherichia coli, Saccharomyces cerevisiae, and Staphylococcus in a volume ratio of 3:1.5:0.7 After inoculation, activate the culture in the seed medium, control the culture temperature at 38°C, shaker speed at 250rpm, culture time for 12h, adjust the pH value to 6.9 with ammonia water; In the fermentation medium, the fermentation culture is carried out in a fermenter, the culture temperature is 38°C, the shaker speed is 250rpm, the culture time is 46h, the pH value is adjusted to 6.9 with ammonia water, and the fermentation liquid containing glucosamine is obtained, and the glucose feeding is controlled during the whole fermentation process. The speed was 4L / h, and the glucosamine content in the fermentation broth was detected to be 79.6g / L.

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Abstract

The invention discloses a method for producing glucosamine through fermentation of escherichia coli. After the escherichia coli, bacillus subtilis and staphylococcus are subjected to seed activation,a mixed fermentation agent is prepared, the mixed fermentation agent is inoculated into a fermentation medium containing glucose and a nitrogen source for constant-temperature shaking table culture, the fermentation process is carried out in a constant-speed material supplementing mode, the fermentation pH is controlled to be 6.8-7.0 through ammonia water, thus fermentation liquid containing the glucosamine is obtained, and the glucosamine is obtained after separation and purification. The fermentation activity of the escherichia coli is improved through the mixed fermentation agent, through addition of cysteine and alanine, the production speed of glutamic acid in the fermentation process can be decreased, and the synthesis rate of the glucosamine is increased.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation, and in particular relates to a method for producing glucosamine by Escherichia coli fermentation. Background technique [0002] Glucosamine (GleN) is an important hexosamine sugar, which is formed by replacing a hydroxyl group of glucose with an amino group. At present, there are two main types of glucosamine on the market, one is glucosamine hydrochloride, and the other is glucosamine sulfate. . Glucosamine Hydrochloride (D-Glucosamine Hydrochloride), molecular formula C 6 h 13 NO 5 ·HCl is a white crystal, odorless, slightly sweet, soluble in water, slightly soluble in methanol, insoluble in ethanol and other organic solvents, it has important physiological functions for the human body, participates in liver and kidney detoxification, exerts anti-inflammatory It has the function of protecting the liver from inflammation and has good curative effect on the treatment of rheuma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P39/00C12P19/26C12R1/19C12R1/125C12R1/44
CPCC12P19/26C12P39/00
Inventor 卢健行马善丽卢建智韩宁张建华姚珊珊
Owner SHANDONG RUNDE BIOTECH CO LTD
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