Method for producing glucosamine through fermentation of escherichia coli

A technology of glucosamine and Escherichia coli, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problems of low production of glucosamine and difficult high-efficiency production, and achieve high-efficiency fermentation production and industrialized production , cost reduction effect
CN110577977AInactive Publication Date: 2019-12-17SHANDONG RUNDE BIOTECH CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SHANDONG RUNDE BIOTECH CO LTD
Publication Date
2019-12-17
Estimated Expiration
Not applicable · inactive patent
Patent Text Reader

Abstract

The invention discloses a method for producing glucosamine through fermentation of escherichia coli. After the escherichia coli, bacillus subtilis and staphylococcus are subjected to seed activation,a mixed fermentation agent is prepared, the mixed fermentation agent is inoculated into a fermentation medium containing glucose and a nitrogen source for constant-temperature shaking table culture, the fermentation process is carried out in a constant-speed material supplementing mode, the fermentation pH is controlled to be 6.8-7.0 through ammonia water, thus fermentation liquid containing the glucosamine is obtained, and the glucosamine is obtained after separation and purification. The fermentation activity of the escherichia coli is improved through the mixed fermentation agent, through addition of cysteine and alanine, the production speed of glutamic acid in the fermentation process can be decreased, and the synthesis rate of the glucosamine is increased.
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Description

technical field

[0001] The invention belongs to the technical field of biological fermentation, and in particular relates to a method for producing glucosamine by Escherichia coli fermentation. Background technique

[0002] Glucosamine (GleN) is an important hexosamine sugar, which is formed by replacing a hydroxyl group of glucose with an amino group. At present, there are two main types of glucosamine on the market, one is glucosamine hydrochloride, and the other is glucosamine sulfate. . Glucosamine Hydrochloride (D-Glucosamine Hydrochloride), molecular formula C 6 h 13 NO 5 ·HCl is a white crystal, odorless, slightly sweet, soluble in water, slightly soluble in methanol, insoluble in ethanol and other organic solvents, it has important physiological functions for the human body, participates in liver and kidney detoxification, exerts anti-inflammatory It has the function of protecting the liver from inflammation and has good curative effect on the treatment of rheuma...

Claims

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